1,720,975 research outputs found
A qualitative and quantitative assay of dihydrofolate reductase in carrot cells resistant to methotrexate.
No full textThe cytochemical pattern and the quantitative assay of DHFR on Daucus carota cell lines sensitive and resistant to methotrexate (MTX) are described. Cytoplasmatic enzyme activity is evidenced as granules of formazan, final reaction product, in the majority of the cells. DHFR activity appears low in Daucus carota cells used as control while in carrot cells resistant to MTX is very high, an observation which parallels previous biochemical studies. These results are supported by quantitative data of DHFR content in a random cell population. A possible correlation between overproduction of DHFR and gene amplification in MTX-resistant cells is discussed but the solution of this problem will depend on the availability of a dhfr plant probe
Tetrahydrofolate dehydrogenase in myelodisplastic syndromes: a qualitative and quantitative study
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Immunohistochemical quantitative analysis of dihydrofolate reductase activity in acute leukemias
No full textDihydrofolate reductase assay in acute lymphoblastic leukaemias by flow cytometry: the role of the enzyme in resistance to methotrexate
No full textDHFR/DNA flow cytometric analysis in animal and plant cells synchronized by aphidicholin
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Acute promyelocitic laeukaemia: detection of dihydrofolate reductase and bromo-deoxyuridine by flow cytometry
No full textEvaluation of serum levels of cytokines and intercellular adhesion molecule-1 (ICAM-1) in astrocytic tumours
No full textDihydrofolate reductase demonstration in proliferating cells labelled with 5-bromo-2-deoxyuridine (BrdU): simultaneous duble staining techniques
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