1,721,165 research outputs found
When a bone marrow exam without cytological abnormalities should be considered truly normal?
No full textBackground: Blood and bone marrow cytological examination represents the unique tool to explore the hematopoietic tissue that can be altered by several intrinsic and extrinsic conditions Cytological assessment of the bone marrow is indicated in several situations including hemogram alterations, hypercalcemia, hyperproteinemia staging for malignancies and monitoring of chemotherapy administration.
Objectives: The purpose of this cytologically retrospective study was to evaluate a consistent number of qualitative and quantitative normal bone marrow exams according to the hematological and clinical-pathological data to judge if this normality is by itself a pathologic state.
Methods: Six hundred and thirteen bone marrow samples were examined using morphological and numerical criteria together with a complete hemogram. The bone marrow cytological examinations were performed after the identification of hematological or clinical alterations such as cytopenia, increased number of cells, positive leishmania serological result and staging of neoplasia.
Results: Of the 613 bone marrow samples evaluated, 85 (14%) were classified as normal. However only 28 (33%) of those cases has a normal hemogram associated, whereas 55 (65%) has one or more cytopenia and 2 (2%) has increased blood cells count.
Conclusions: From this retrospective study emerge that cytological bone marrow examinations without any morphological or numerical abnormalities are often associated with altered hematological exams and for this reason they should not be considered normal and should lead to other deepened investigations and sometimes to a second cytological bone marrow exam after some days
Quantification of zolpidem in canine plasma
Full text linkProblem statement: Zolpidem is a non-benzodiazepine hypnotic agent currently used in human medicine. In contrast to benzodiazepines, zolpidem preferentially binds with the GABAA complex πvariant 1 receptors while poorly interacting with the other πvariant receptor complexes. Recent studies have suggested that ZP may be used to initiate sedation and diminish severe anxiety responses in dogs. The aim of the present study is to develop and validate a new HPLC-FL based method to quantify zolpidem in canine plasma. Approach: Several parameters both in the extraction and in the detection method were evaluated. The applicability of the method was determined by administering zolpidem to one dog. Results: The final mobile phase was acetonitrile: KH2PO4 (15 mM; pH 6.0) 40:60 v/v, with a flow rate of 1 mL min-1 and excitation and emission wave lengths of 254 and 400 nm, respectively. The best extraction solvent was CH2Cl2:Et2O (3:7 v/v), this gave recoveries ranging from 83-95%. The limit of quantification was 1 ng mL-1. The chromatographic runs were specific with no interfering peaks at the retention times of the analyte. The other validation parameters were in agreement with the EMEA. Conclusion/Recommendations: This method (extraction, separation and applied techniques) is simple and effective. This technique may have applications for pharmacokinetic or toxicological studies. © 2012 Science Publications
Hemophilia A (Factor VIII Deficiency) in a Family of Belgian Shepherd Malinois Dogs Bred in Italy
No full textHemato-biochemical and serological evaluation in canine blood donors receiving Canileish® vaccine
No full textImmune-mediated thrombocytopenia and hemolytic anemia (Evans' syndrome) in a horse
No full textA clinical case of thrombocytopenia and anemia in a horse with a presumptive diagnosis of immune-mediated origin (Evans' syndrome) is reported. The clinical signs, diagnostic approach, laboratory features, therapy, clinical course, and gross and histopathological examinations are described
Effusion in the cat: classification of 396 fluids according to a problem-oriented scheme
No full textAn effusion is an abnormal accumulation of fluid
within a body cavity (abdominal, thoracic, pericardial) and it
is a common sign of several disorders in cats. Examination
and classification of effusions can help to establish the cause
of disease and to provide useful information for diagnosis
and prognosis. The aim of this study was to classify feline
effusions according to a problem-oriented approach in order
to reduce the diagnostic differential. Three hundred and
ninety-six thoracic and abdominal feline effusions were
classified using both cytological criteria and refractometrical
estimates of total protein concentration. This study revealed
the high prevalence of lymphorrhagic effusions (23.2 %)
and the rarity of bile peritonitis (0.3 %), mesothelial hyperplasia (0.5 %), and eosinophilic exudates (0.8 %) in the cat.
Our problem-oriented classification scheme appears to be
clinically useful, providing a reduced diagnostic differential,
and drives the clinician to perform additional diagnostic
tests
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