1,720,980 research outputs found
The "VGF" protein in rat adenohypophysis: sex differences and changes during the estrous cycle and after gonadectomy
No full textGene expression and cell localization of the neuroendocrine protein VGF were studied in the rat anterior pituitary. In females, four antisera against nonoverlapping regions of VGF immunostained a small number of lactotropes and many gonadotropes. In the latter cells, VGF immunoreactivity was localized to a subpopulation of secretory granules. Distinct changes were seen after estrus, with a significant increase in VGF messenger RNA (whole pituitary), whereas VGF immunostaining was strikingly reduced in gonadotropes and somewhat more abundant in lactotropes. In male rats, gene expression was low, and immunoreactivity was restricted to a few lactotropes. After castration or ovariectomy, VGF messenger RNA was high, and VGF immunoreactivity was abundant in gonadotropes. Selective localization and cyclic modulation suggest involvement of the VGF gene product(s) in pituitary gonadotrope and/or lactotrope function
EXPERIMENTAL ßß'-IMINODIPROPIONITRILE NEUROPATHY: NEUROFILAMENT PROFILE OF SENSORY, MOTOR AND AUTONOMIC NERVES AS SEEN BY IMMUNOCYTOCHEMISTRY ON WHOLE-MOUNT PREPARATIATIONS
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Genetic effects of vicryl® on fibroblast primary culture
No full textVicryl® (polyglactin 910) is an absorbable, synthetic, usually braided suture, indicated for soft tissue approximation and ligation. Vicryl® has a special coating for minimizing friction, easing passage through tissue and easy knot tie down. It is synthetic for minimal tissue reaction. Fibroblasts are the main cells of connective tissue that synthesize extracellular matrix. In this work, we tried to judge the action of Vicryl® on fibroblasts behaviour. We evaluated the expression levels of some adhesion and traction-resistance related genes (ELN, DSP, FN1, FBN1, ITGB1, ITGA1, ITGA5, ITGA2, COL1A1, COL3A1) by using real time Reverse Transcription-Polymerase Chain Reaction (real time RT-PCR). All but 2 genes resulted up-regulated after 48 h of treatment. Our preliminary results point out the potential of Vicryl® as a biocompatible and regenerative tool in medicine.Vicryl (R) (polyglactin 910) is an absorbable, synthetic, usually braided suture, indicated for soft tissue approximation and ligation. Vicryl (R) has a special coating for minimizing friction, easing passage through tissue and easy knot tie down. It is synthetic for minimal tissue reaction. Fibroblasts are the main cells of connective tissue that synthesize extracellular matrix. In this work, we tried to judge the action of Vicryl (R) on fibroblasts behaviour. We evaluated the expression levels of some adhesion and traction- resistance related genes (ELN, DSP, FN1, FBN1, ITGB1, ITGA1, ITGA5, ITGA2, COL1A1, COL3A1) by using real time Reverse Transcription-Polymerase Chain Reaction (real time RT-PCR). All but 2 genes resulted up-regulated after 48 h of treatment. Our preliminary results point out the potential of Vicryl (R) as a biocompatible and regenerative tool in medicine
Trauma, alcohol and illicit drugs. Intrahospital surveillance
No full textThe role of illicit drugs in determining trauma is frequently reported, even if there is a relevant variability in the frequency of the causal connections between illicit drugs and traumatic events. The aim of this study was to analyze in a systematic way the severity of trauma, the type of commonly used drugs and the possible connections with trauma in injured patients admitted to first aid department
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