1,721,110 research outputs found
Scoring surgical skill in Robotic Prostatectomy as adherence to the surgical plan: proposal for a new tool (ScAPSA)
No full textThe plethora of instruments (trifecta, pentafecta, etc.) used to evaluate the outcomes of robotic prostatectomy (RARP) has recently been subjected to criticism. In this paper, a novel approach called ScAPSA (Scoring Adherence to Prostatic Surgical Aims) is proposed to assess surgical proficiency, considering surgical success as perfect adherence to a correct surgical plan, and not related solely to clinical outcomes
Postpartum maternal death associated with undiagnosed Hodgkin's lymphoma
No full textThe authors describe the case of a 28-year-old patient who died from an extensive intracerebral haemorrhage due to disseminated intravascular coagulation (DIC) which occurred a few days after delivery. Access to an emergency department of gynaecology on three separate occasions in the three days preceding hospitalization made judicial evaluation of the case necessary. The case was studied with a methodological approach based on the following steps: (1) examination of clinical records; (2) autopsy; (3) study of the placenta; (4) anatomo-histopathological observations concerning particular organs and structures potentially involved in postpartum maternal death; and (5) evaluation of physicians' behaviour, in the light of necroscopic findings and a review of the literature. The causes of death most frequently described in the postpartum period were excluded; a mediastinal nodular sclerosing Hodgkin's lymphoma with transdiaphragmatic diffusion, not diagnosed in life, was demonstrated. The cause of death was identified as intracerebral haemorrhage following DIC, Hodgkin's disease and recent delivery by caesarean section. Analysis of the physicians' conduct, together with a review of the literature, revealed a medical error. However, no causal relationship between the error and the death of the patient was considered to exist. The interest of the case lies in the unusual cause of DIC discussed in relation to a hypothesis of obstetric-gynaecological liability
Clear cell meningioma in a 22-month-old male: update after five years
No full textWe report a case of spinal clear-cell meningioma occurring in a 22-month-old male who presented a right limp and then refused to walk. Spinal magnetic resonance imaging demonstrated a large, intradural tumor from T(11) to L(4), which was totally excised. The patient's postoperative recovery was uneventful and 5 months after surgery he began walking again. The latest follow-up magnetic resonance imaging of the brain and spine, obtained 42 months after diagnosis, was negative for tumor recurrence. Though clear-cell meningioma is a rare form of meningioma, it should be considered in the differential diagnosis of any space-occupying lesion of the spine arising in very young children. Complete surgical removal is necessary because it is potentially aggressive and may recur. After surgery, an accurate follow-up is warranted.
Copyright 2001 S. Karger AG, Base
Somatic Embryogenesis from stigmas and styles of Grapevine
No full textAn in vitro protocol has been developed for callus induction, somatic embryogenesis, and plant regeneration from stigma-style Culture of grapevine. Four different grapevine cultivars (Vitis vinifera L.; cvs. 'Bombino Nero,' 'Greco di Tufo,' 'Merlot,' and 'Sangiovese') were tested. Explants were cultured on Nitsch and Nitsch medium (NN) supplemented with various combinations of 6-benzylaminopurine (BA; 4.5 and 9.0 mu M) and beta-naphthoxyacetic acid (NOA; 5.0 and 9.9 mu M). Sucrose (88 mM) was used as the carbon source. Somatic embryogenesis was induced within 3-7 mo. after culture initiation. Even though explants of different origin (unfertilized ovules and anthers) regenerated somatic embryos, the higher embryogenic potential was observed in stigma and style explants, with the exception of 'Merlot,' which regenerated somatic embryos only from unfertilized ovules. The percentages of stigma-style explants producing somatic embryos was 7% in 'Bombino Nero' (cultured on NN medium supplemented 9.0 mu M BA and 9.9 mu M NOA), 14% in 'Greco di Tufo' (4.5 mu M BA and 9.9 mu M NOA), and 8% in 'Sangiovese' (9.0 mu M BA and 9.9 mu M NOA). The presence of growth regulators (BA and NOA) in the medium was essential for induction of somatic embryogenesis. Plants were regenerated on hormone-free NN medium containing 88 mM sucrose
An unusual case of malignant pilocytic astrocytoma occurring in the eye.
No full textPilocytic astrocytoma is a central nervous system neoplasia that arises during pediatric age. Only few cases have been documented in patients older than 50 years old. It is a low-grade lesion that can rarely undergo malignant changes presenting the histologic features of a high-grade glioma. We report a case of a pilocytic astrocytoma arising in the eyeball of a 53-year-old man affected by glaucoma that underwent malignant evolutio
Tecniche di risanamento nella selezione sanitaria della vite
No full textIl lavoro riporta i risultati di esperienze di risanamento attuate tramite tecniche di coltura in vitro di apici vegetativi e apici meristematici su biotipi in fase di selezione clonale. Gli accertamenti sanitari sono stati condotti con test ELISA e/o PCR per l’esame dei virus sulle plantule in vitro; saggi biologici sono stati eseguiti su alcune piante adulte. I successi del risanamento variano in funzione delle tecniche adottate e dei virus esaminati: la sola coltura in vitro ha permesso di eliminare tutti i virus su circa il 20% degli espianti, mentre con nuovi espianti operati su materiali sottoposti o meno ad interventi di termoterapia in vitro le percentuali di risanamento sono di gran lunga aumentate. Le maggiori difficoltà di guarigione si riscontrano per il virus associato a rupestris stem pitting (GRSPaV).
The paper refers the results of virus elimination obtained by in vitro shoot tip and meristem tip culture of grapevine biotypes in clonal selection. Sanitary tests were carried out with ELISA and/or PCR assays for virus identification in plants cultivated in vitro; biological indexing were performed on some plants replanted in the field. Virus elimination depends on adopted methods and on the virus: in vitro meristem tip culture allowed to eliminate the viruses from more than 20% of the shoots, while taking new shoot tips from plant material treated or not treated with in vitro heat therapy gave much better results. The most difficult virus to eliminate was the rupestris stem pitting associated virus (GRSPaV)
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