1,721,090 research outputs found
Studio dei fattori di crescita coinvolti nello sviluppo tissutale e nella deposizione di matrice extracellulare del porifero Chondrosia reniformis (Nardo, 1847) con metodi immunoistochimici e di biologia molecolare.
No full textTissue repair is an adaptive and common metazoan response characterized by various cellular mechanisms and complex signalling networks that involve several growth factors and cytokines. In higher animals, the transforming growth factor-β superfamily members (TGF-β SF) signalling plays a fundamental role in wound healing. Aim of this thesis was to assess the involvement of TGF-β SF members in lower invertebrate tissue regeneration. To achieve this result, sequence that code for putative TGF ligands and receptors were isolated from the transcriptome of a marine sponge Chondrosia reniformis, transcriptome that was previously obtained in the laboratory hosting the research object of this doctorate’s work.
C. reniformis is a marine Demospongiae, without siliceous spicules, but with a robust collagen scaffold. Since its identification as a true poriferous by Giovanni Domenico Nardo in the 1847, it has been the subject of study by zoologists, ecologists and more recently biotechnologists interested in several of its biological peculiarities.
In the first part of the research we identified six transcripts that coded for TGF superfamily ligands and two sequences that coded for TGF superfamily receptors. Phylogenetically, the TGF ligands of C. reniformis could not be grouped in a TGF-β SF clades and consequently these ligands presumably evolved independently, while the TGF receptors clustered in the Type I receptor group.
During the research activities we examined the gene expression of these transcripts and the cell regeneration processes from a microscopic morphological point of view in a particular type of sponge regenerating tissue explants called fragmorph. The fragmorphs are essentially coring, in our case of 9 millimeters in diameter, made perpendicularly to the upper face of the sponge.
The data obtained indicated that three ligands (TGF-1, -3 and -6) could be involved in staminal cell maintenance, since they were mainly expressed during early regeneration, while two (TGF-4 and TGF-5) were considered pro-differentiating factors, since they were strongly upregulated during late regeneration. In presence of 0.1mM SB431542, a strong TGF receptors inhibitor, the exopinacoderm restoring of the fragmorph resulted blocked, attesting the functional involvement of TGF-pathway in tissue regeneration also in these early evolved animals
Immunohistochemical and lectin-based approach to Acerentomon sp. anatomy (Protura: Acerentomidae).
No full textThe present study describes for the first time in Acerentomon sp. the distribution of glutamate decarboxylase-like immunoreactivity. The presence of this GABA-synthetizing enzyme was described in: pharynx wall, cephalic muscles, posterior part of the supraesophageal ganglion, abdominal glands, neuropil near to the posterior ganglion, cuticular muscle insertion and in sensilla. Furthermore the use of fluorochrome-conjugated lectins was tested in order to visualize taxonomic features (like pores and setae) on whole mount fixed Protura specimens. The development of a lectin-based technique could render more precise the species determination, allowing a more accurate identification
Body Composition and Fatty Acid Profile of Carps under the Influence of Rice Polish and Pond Fertilization
No full textThe experiment was conducted in two earthen ponds to investigate the body composition, and fatty acid profile of carps along with growth performance by using rice polish as fish feed with urea and ammonium nitrate fertilization of pond. Silver carp (Hypophthalmichthys molitrix), Rohu (Labeo rohita) and Mori (Cirrhinus mrigala) were stocked in the ratio of 1:2:1 respectively with total number of 40 fishes in each pond. Pond 1 was treated with urea and rice polish while pond 2 was treated with ammonium nitrate and rice polish at the rate of 0.3g Nitrogen/100g of wet body weight of fish daily. There was found highly significant difference between species and ponds in terms of growth. Highest growth in terms of gain in body weight was shown by H. molitrix in both ponds i.e. 399.0g in pond 1 and 299.9g in pond 2. Net fish production of pond 1 remained as 1311.3 kg/ha/year and pond 2 was 1104.2 kg/ha/year. Pond 1 showed 1.5 times greater fish production than pond 2. Proximate analysis of the fish meat showed that there was no significant difference in body composition of three species in both the ponds. Highest protein contents (18.04 %) were found in the meat of C. mrigala in P1 and lowest protein contents (17.16 %) were in the meat of H. molitrix in P2. There was also non-significant difference between three species in terms of fatty acid profile. The overall contents of fatty acids were highest in L. rohita as compared to other two species. Saturated fatty acids were highest in L. rohita whereas monounsaturated fatty acids were highest in C. mrigala
Involvement of acetyl choline in settlement of Balanus amphitrite.
No full textAcetylcholinesterase (AChE, the lythic enzyme of acetylcholine) activity was detected, for the first time, by biochemical and histoenzymological methods, in the thoracic muscles, gut wall and cement gland. The immunodetection of choline acetyltransferase-like (ChAT) molecules in the same area and in the neuropil of the central nervous system suggests the presence of a cholinergic innervation, and the involvement of acetylcholine in muscular contraction and cement gland exocytosis. The binding of FITC-conjugate alpha-bungarotoxin in the cement gland cells confirms the latter hypothesis. Acetylcholine involvement in the settlement process was also investigated by laboratory tests employing cholinergic antagonists and agonists. An increase of available acetylcholine due to the partial inhibition of AChE activity produced an increase in cyprid settlement. The data presented support the hypothesis that acetylcholine has a neurotransmitter/neuromodulator role in settlement and adhesion of barnacle cyprid
Morfologia della lingua e istochimica delle ghiandole linguali negli odontoceti del Mar Ligure
No full text- …
