1,721,106 research outputs found
Identification of two homologous mitochondrial DNA sequences which bind strongly and specifically to a mitochondrial protein of Paracentrotus lividus
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Identification by in organello footprinting of protein contact sites and of single-stranded DNA sequences in the regulatory region of rat mitochondrial DNA
No full textHindlimb suspension unloaded and reloaded conditions affect mtDNA and TFAM content of rat hindlimb skeletal muscles
No full textAcetyl-L-carnitine feeding to unloaded rats triggers in soleus muscle the coordinated expression of genes involved in mitochondrial biogenesis
No full textThe expressional profile of mitochondrial transcripts and of genes involved in the mitochondrial biogenesis pathway induced by ALCAR daily supplementation in soleus muscle of control and unloaded 3-month-old rats has been analyzed. It has been found that ALCAR treatment is able to upregulate the expression level of mitochondrial transcripts (COX I, ATP6, ND6, 16 S rRNA) in both control and unloaded animals. Interestingly, ALCAR feeding to unloaded rats resulted in the increase of transcript level for master factors involved in mitochondrial biogenesis (PGC-1alpha, NRF-1, TFAM). It also prevented the unloading-induced downregulation of mRNA levels for kinases able to transduce metabolic (AMPK) and neuronal stimuli (CaMKIIbeta) into mitochondrial biogenesis. No significant effect on the expressional level of such genes was found in control ALCAR-treated rats. In addition, ALCAR feeding was able to prevent the loss of mitochondrial protein content due to unloading condition. Correlation analysis revealed a strong coordination in the expression of genes involved in mitochondrial biogenesis only in ALCAR-treated suspended animals, supporting a differentiated effect of ALCAR treatment in relation to the loading state of the soleus muscle. In conclusions, we demonstrated the ability of ALCAR supplementation to promote only in soleus muscle of hindlimb suspended rats an orchestrated expression of genes involved in mitochondrial biogenesis, which might counteract the unloading-induced metabolic changes, preventing the loss of mitochondrial proteins
NUCLEOTIDE SEQUENCE AND GENE ORGANIZATION OF MITOCHONDRIAL DNA FROM PARACENTROTUS LIVIDUS
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Mitochondrial DNA mutations in RRF of healthy subjects of different age
No full textTo obtain information on the mechanisms responsible of the generation of ragged red fibers (RRF) during aging, we analyzed the mitochondrial
genotype of single skeletal muscle fibers of healthy individuals having an age comprised between 45 and 92 years. The sequencing
of the D-loop region showed many sequence changes with respect to the Cambridge reference sequence (CRS), in both RRF and normal
fibers. These changes were more abundant in RRF and their number increased between 50 and 60, and 61 and 70 years and then remained
approximately constant. The analysis of the sequence changes showed that each subject contained one or more changes associated to RRF in
positions of D-loop region that either do not change or that change very rarely. In general the same type of RRF-associated change was not
found in more than one individual; exceptions were changes in positions 189, 295, 374 and 514, detected in 20–50% of analyzed subjects.
In particular the A189G age-associated mutation was found only in old individuals and prevalently in RRF. Sequencing of other two mtDNA
regions showed no relevant changes in the 16S/ND1 region and two RRF-associated original mutations, G5847A and A5884C, in two very
conserved positions of tRNATyr. These results indicate that each subject has its own pattern of RRF-associated mutations in both coding and
non-coding region of human mtDNA
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