177 research outputs found
Repertorium der Preußisch-Brandenburgischen Landesgesetze : welche in den ältern und neuern Edicten-Sammlungen, den Beiträgen zur juristischen Litteratur und den Annalen der Gesetzgebung in den Preußischen Staaten enthalten sind : mit Hinweisung auf das allgemeine Gesetzbuch nach alphabetischer Materienfolge für Justizbediente
No full textentworfen von P. J. G. Hoffmann Königl. Neumärk. RegierungsrathVorlageform des Erscheinungsvermerks: Cüstrin, gedruckt bei C. G. Trowitzsch Königl. Hofbuchdrucker. 1793
An unusual RNA recognition motif acts as a scaffold for multiple proteins in the pre-mRNA retention and splicing complex.
No full textThe yeast pre-mRNA retention and splicing complex counteracts the escape of unspliced pre-mRNAs from the nucleus and activates splicing of a subset of Mer1p-dependent genes. A homologous complex is present in activated human spliceosomes. In many components of the spliceosome, RNA recognition motifs (RRMs) serve as versatile protein-RNA or protein-protein interaction platforms. Here, we show that in the retention and splicing complex, an atypical RRM of the Snu17p (small nuclear ribonucleoprotein-associated protein 17) subunit acts as a scaffold that organizes the other two constituents, Bud13p (bud site selection 13) and Pml1p (pre-mRNA leakage 1). GST pull-down experiments and size exclusion chromatography revealed that Snu17p constitutes the central platform of the complex, whereas Bud13p and Pml1p do not interact with each other. Fluorimetric structure probing showed the entire Bud13p and the N-terminal third of Pml1p to be natively disordered in isolation. Mutational analysis and tryptophan fluorescence confirmed that a conserved tryptophan-containing motif in the C terminus of Bud13p binds to the core RRM of Snu17p, whereas a different interaction surface encompassing a C-terminal extension of the Snu17p RRM is required to bind an N-terminal peptide of Pml1p. Isothermal titration calorimetry revealed 1: 1 interaction stoichiometries, large negative binding entropies, and dissociation constants in the low nanomolar and micromolar ranges for the Snu17p-Bud13p and the Snu17p-Pml1p interactions, respectively. Our results demonstrate that the noncanonical Snu17p RRM concomitantly binds multiple ligand proteins via short, intrinsically unstructured peptide epitopes and thereby acts as a platform that displays functional modules of the ligands, such as a forkhead-associated domain of Pml1p and a conserved polylysine motif of Bud13p.Max-Planck-Societ
Three New Potent HIV-1 Inhibitors from Myxobacteria
No full textThree novel compounds, namely, phenoxan, phenalamide A
1
, and thiangazole, were found to suppress HIV-1 replication in cell cultures. The compounds were discovered by screening crude extracts from myxobacteria and were isolated from two strains of Polyangium sp. and a strain of Myxococcus stipitatus. Their structures have been elucidated. The cytotoxic concentrations for MT-4 cells were 6.6 μM for phenoxan, 102 μM for phenalamide A
1
, and 4.7 μM for thiangazole. Phenoxan inhibited the HIV-1-dependent cell death at concentrations of as low as 6.6 nM. Phenalamide A
1
could prevent the HIV-1 infection of MT-4 cells even at concentrations of 1.02nM, and thiangazole at 4.7 pM. In our assay thiangazole is at least 100 times more active than AZT. The compounds could not prevent syncythia formation induced by HIV-1. However, like HEPT (Baba et al., 1989; Miyasaka et al., 1989) and TIBO (Pauwels et al., 1990) derivatives they are highly specific since they could not interfere with HIV-2ben dependent MT-4 cell death. HIV-1 RT activity was inhibited by 50% by 376 μM phenoxan, 386 μM phenalamid A
1
, or 263 μM thiangazole. Since these concentrations are approximately 50000 times higher than their minimum concentrations active in cell cultures, RT-inhibition does not appear to be the major mechanism of HIV-inhibition of the new agents
Crystal structure of the Pml1p subunit of the yeast precursor mRNA retention and splicing complex.
No full textThe precursor mRNA retention and splicing (RES) complex mediates nuclear retention and enhances splicing of precursor mRNAs. The RES complex from yeast comprises three proteins, Snu17p, Bud13p and Pml1p. Snu17p acts as a central platform that concomitantly binds the Bud13p and Pml1p subunits via short peptide epitopes. As a step to decipher the molecular architecture of the RES complex, we have determined crystal structures of full-length Pml1p and N-terminally truncated Pml1p. The first 50 residues of full-length Pml1p, encompassing the Snu17p-binding region, are disordered, showing that Pml1p binds to Snu17p via an intrinsically unstructured region. The remainder of Pml1p folds as a forkhead-associated (FHA) domain, which is expanded by a number of noncanonical elements compared with known FHA domains from other proteins. An atypical N-terminal appendix runs across one beta-sheet and thereby stabilizes the domain as shown by deletion experiments. FHA domains are thought to constitute phosphopeptide-binding elements. Consistently, a sulfate ion was found at the putative phosphopeptide-binding loops of full-length Pml1p. The N-terminally truncated version of the protein lacked a similar phosphopeptide mimic but retained an almost identical structure. A long loop neighboring the putative phosphopeptide-binding site was disordered in both structures. Comparison with other FHA domain proteins suggests that this loop adopts a defined conformation upon ligand binding and thereby confers ligand specificity. Our results show that in the RES complex, an FHA domain of Pml1p is flexibly tethered via an unstructured N-terminal region to Snu17p. (C) 2008 Elsevier Ltd. All rights reserved.Max Planck Societ
Zur Versorgung der Stadt Berlin mit Trinkwasser
No full textZUR VERSORGUNG DER STADT BERLIN MIT TRINKWASSER
Zur Versorgung der Stadt Berlin mit Trinkwasser / Schramke, G. (Public Domain) ( - )
Title page ( - )
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Auszug aus dem ad XIV. angeregten Plane zur Versorgung der Stadt Berlin mit reinem und gesundem Wasser und zur Bewässerung der Straßen (11)
Karte: Plan von der Wasserregion und dem Laufe des Aquaduct's zur Versorgung der Stadt Berlin mit reinem und gesundem Wasser und zur Bewässerung der Strassen ( -
M. E. G. Tietzens geistliche Wasserquelle woraus zu schoepfen taegliche Morgen- und Abend- Segen ... wie auch Kraeftige Seufzer aller Nothleidenden und Herzliche Gebete in Wettersnoth,. ..
No full textCatalog Arabischer Manuscripte / In Damascus gesammelt von J. G. Wetzstein
No full text[2. Ex. durch Ankauf in den Besitz der Königl. Bibliothek zu Berlin übergegangen
Structural basis for reversible photoswitching in Dronpa.
No full textDronpa is a novel GFP-like fluorescent protein with exceptional light-controlled switching properties. It may be reversibly switched between a fluorescent on-state and a nonfluorescent off-state by irradiation with light. To elucidate the molecular basis of the switching mechanism, we generated reversibly switchable Dronpa protein crystals. Using these crystals we determined the elusive dark-state structure of Dronpa at 1.95-A resolution. We found that the photoswitching results in a cis-trans isomerization of the chromophore accompanied by complex structural rearrangements of four nearby amino acid residues. Because of this cascade of intramolecular events, the chromophore is exposed to distinct electrostatic surface potentials, which are likely to influence the protonation equilibria at the chromophore. We suggest a comprehensive model for the light-induced switching mechanism, connecting a cascade of structural rearrangements with different protonation states of the chromophore
Kommentar zu den Königl. Preußischen Stempel-Gesetzen; enthaltend das Gesetz wegen der Stempelsteuer vom 7. März 1822, den Stempel-Tarif von demselben Tage, und die in Bezug auf beide späterhin ergangenen gesetzlichen Bestimmungen und Verordnungen, so wie Bemerkungen für den praktischen Gebrauch, für Beamte, Geschäftsmänner und Privatleute, nebst Tabellen über die Berechnung der Stempelsätze
No full text... enthaltend die seit dem Erscheinen des zweiten Bandes ergangenen gesetzlichen Bestimmungen und Verordnungen, so wie auch einige früher erlassene. Nebst einer chronologischen Übersicht ... und einem vollständigen Sach-Register über alle drei Bände; für Beamte, Geschäftsmänner und Privatleute; nebst Tabellen über die Berechnung der Stempelsätze
No full text- …
