58 research outputs found
Gaining insight into the maturation of iron-sulfur (FeS) clusters in staphylococcus aureus by determining the role of DUF59 protein, SufT
No full textStaphylococcus aureus is a serious mammalian pathogen. For Staphylococcus aureus to successfully inflict pathogenesis upon a host, it is imperative for it to acquire and effectively utilize iron (Fe). Once Fe is internalized, S. aureus utilizes the SUF system to assemble small inorganic cofactors called iron-sulfur (FeS) clusters. FeS clusters have a wide variety of functions in cells, thus, defective FeS cluster assembly results in global metabolic defects. In addition, FeS cluster synthesis and the assembly of FeS proteins is essential in S. aureus and a number of alternate bacterial pathogens suggesting that it is a viable antimicrobial target. Proteins containing DUF59 domains have roles in FeS cluster assembly and are found throughout Eukarya, Bacteria and Archaea. However, the function of DUF59 remains unknown in S. aureus. We have identified the S. aureus SufT, which is composed solely of the DUF59 domain and demonstrated that it has a role in the maturation of iron-sulfur (FeS) proteins.M.S.Includes bibliographical referencesby Shiven K. Bhat
Interação entre as substâncias húmicas de sedimentos e metais potencialmente tóxicos: um estudo na Bacia Hidrográfica do Turvo/Grande
No full textEste trabalho teve como objetivo estudar a interações das Substâncias Húmicas (SH) com os metais cobre(II), cromo(III) e cromo(VI) por meio de dois métodos, o da supressão da fluorescência molecular e o sistema de ultrafiltração com fluxo tangencial (SUFT). As SH foram caracterizadas por técnicas espectroscópicas na região do infravermelho e do ultravioleta-visível e por fluorescência molecular. Foram realizadas duas coletas de sedimentos, uma no período de chuva e outra no de seca, sendo coletados em três rios da Bacia Hidrográfica do Turvo/Grande, Rio Preto, Rio Turvo e Rio Grande. O sedimento foi coletado com o auxilio de uma draga manual do tipo Van Veen. As SH foram extraídas de acordo com metodologia oficial sugerida pela Sociedade Internacional de Substâncias Húmicas (SISH). A constante de estabilidade condicional (Kc) foi determinada por meio da supressão da fluorescência e variou de 5,6x10-3 – 1,23 em período chuvoso para o íons cobre, e de 5,5x10-3 – 8,7x10-2 para o período de seca. Quando a Kc foi determinada para o complexo SH-Cr(III) valores de 1,9x10-1-5,2x10-1 e 2,1x10-1-3,5x10-1, foram determinados para o período de chuva e de seca, respectivamente. Por fim a Kc foi determinada para a interação das SH com Cr(VI), de forma geral os valores de Kc foram maiores para o período de chuva. A capacidade complexante (CC) determinada por meio do SUFT para a interação das SH com cobre(II) teve valores que variaram de 4,0x10-1 – 6,3x10-1 mmol Cu g-1 SH e de 1,4x10-1 – 5,3x10-1 mmol Cu g-1 SH para período de chuva e seca, respectivamente. Para o Cr(III) a CC variou de 2,1x10-1 – 9,7x10-1 e de 2,6x10-1 – 8,4x10-1 mmol Cr g-1 SH para o período de chuva e seca, respectivamente. Quando a interação das SH com o Cr(VI) foi estudada por meio do SUFT só foi possível calcular a CC para os pontos...This work aimed to study the interactions of humic substances (HS) with copper(II), chromium(III) and chromium(VI), using by two methods, the suppression of the molecular fluorescence and tangential flow ultrafiltration sytem (TFUS). The HS were characterized by spectroscopic thecniques in the infrared and ultraviolet/visible region and molecular fluorescence. There were two sediment samples, one during the wet season and another the dry, being these sediments collected from the three Rivers of the Turvo/Grande drainage basin, Preto river, Turvo river and Grande river. The collection of sediment was carried out with the aid of a manual Van Veen type dredge. The HS were extracted according to the official methodology suggested by the International Humic Substances Society (IHSS). The conditional stability constant (Kc) was determined by suppressing fluorescence and ranged from 5,6x10-3 – 1,23 in the wet season for the copper ions, and of the 5,5x10-3 – 8,7x10-2 to the dry season. When Kc was determined for the complex HS-Cr(III) values of 1,9x10-1-5,2x10-1 e 2,1x10-1-3,5x10-1, were determined for the wet and dry seasons, respectively. Finally, the Kc was determined for the interactions oh HS with the Cr(VI) in geral the Kc values were higher for the wet season. Complexant capacity (CC) was determined by means of TFUS for the interactions of the HS with Copper(II) the values ranged from 4,0x10-1 – 6,3x10-1 mmol Cu g-1 HS and of 1,4x10-1 – 5,3x10-1 mmol Cu g-1 HS for a wet and dry season, respectively. For the Cr(III) CC ranged from 2,1x10-1 – 9,7x10-1 and from 2,6x10-1 – 8,4x10-1 mmol Cr g-1 HS for the wet and dry period, respectively. When the interactions oh HS with Cr(VI) was studied by means of TFUS was only possible to calculate the CC for the points NRP and CAPRP, as for others... (Complete abstract click electronic access below)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES
Interação entre as substâncias húmicas de sedimentos e metais potencialmente tóxicos: um estudo na Bacia Hidrográfica do Turvo/Grande
No full textEste trabalho teve como objetivo estudar a interações das Substâncias Húmicas (SH) com os metais cobre(II), cromo(III) e cromo(VI) por meio de dois métodos, o da supressão da fluorescência molecular e o sistema de ultrafiltração com fluxo tangencial (SUFT). As SH foram caracterizadas por técnicas espectroscópicas na região do infravermelho e do ultravioleta-visível e por fluorescência molecular. Foram realizadas duas coletas de sedimentos, uma no período de chuva e outra no de seca, sendo coletados em três rios da Bacia Hidrográfica do Turvo/Grande, Rio Preto, Rio Turvo e Rio Grande. O sedimento foi coletado com o auxilio de uma draga manual do tipo Van Veen. As SH foram extraídas de acordo com metodologia oficial sugerida pela Sociedade Internacional de Substâncias Húmicas (SISH). A constante de estabilidade condicional (Kc) foi determinada por meio da supressão da fluorescência e variou de 5,6x10-3 – 1,23 em período chuvoso para o íons cobre, e de 5,5x10-3 – 8,7x10-2 para o período de seca. Quando a Kc foi determinada para o complexo SH-Cr(III) valores de 1,9x10-1-5,2x10-1 e 2,1x10-1-3,5x10-1, foram determinados para o período de chuva e de seca, respectivamente. Por fim a Kc foi determinada para a interação das SH com Cr(VI), de forma geral os valores de Kc foram maiores para o período de chuva. A capacidade complexante (CC) determinada por meio do SUFT para a interação das SH com cobre(II) teve valores que variaram de 4,0x10-1 – 6,3x10-1 mmol Cu g-1 SH e de 1,4x10-1 – 5,3x10-1 mmol Cu g-1 SH para período de chuva e seca, respectivamente. Para o Cr(III) a CC variou de 2,1x10-1 – 9,7x10-1 e de 2,6x10-1 – 8,4x10-1 mmol Cr g-1 SH para o período de chuva e seca, respectivamente. Quando a interação das SH com o Cr(VI) foi estudada por meio do SUFT só foi possível calcular a CC para os pontos...This work aimed to study the interactions of humic substances (HS) with copper(II), chromium(III) and chromium(VI), using by two methods, the suppression of the molecular fluorescence and tangential flow ultrafiltration sytem (TFUS). The HS were characterized by spectroscopic thecniques in the infrared and ultraviolet/visible region and molecular fluorescence. There were two sediment samples, one during the wet season and another the dry, being these sediments collected from the three Rivers of the Turvo/Grande drainage basin, Preto river, Turvo river and Grande river. The collection of sediment was carried out with the aid of a manual Van Veen type dredge. The HS were extracted according to the official methodology suggested by the International Humic Substances Society (IHSS). The conditional stability constant (Kc) was determined by suppressing fluorescence and ranged from 5,6x10-3 – 1,23 in the wet season for the copper ions, and of the 5,5x10-3 – 8,7x10-2 to the dry season. When Kc was determined for the complex HS-Cr(III) values of 1,9x10-1-5,2x10-1 e 2,1x10-1-3,5x10-1, were determined for the wet and dry seasons, respectively. Finally, the Kc was determined for the interactions oh HS with the Cr(VI) in geral the Kc values were higher for the wet season. Complexant capacity (CC) was determined by means of TFUS for the interactions of the HS with Copper(II) the values ranged from 4,0x10-1 – 6,3x10-1 mmol Cu g-1 HS and of 1,4x10-1 – 5,3x10-1 mmol Cu g-1 HS for a wet and dry season, respectively. For the Cr(III) CC ranged from 2,1x10-1 – 9,7x10-1 and from 2,6x10-1 – 8,4x10-1 mmol Cr g-1 HS for the wet and dry period, respectively. When the interactions oh HS with Cr(VI) was studied by means of TFUS was only possible to calculate the CC for the points NRP and CAPRP, as for others... (Complete abstract click electronic access below)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES
Interação entre as substâncias húmicas de sedimentos e metais potencialmente tóxicos: um estudo na Bacia Hidrográfica do Turvo/Grande
No full textEste trabalho teve como objetivo estudar a interações das Substâncias Húmicas (SH) com os metais cobre(II), cromo(III) e cromo(VI) por meio de dois métodos, o da supressão da fluorescência molecular e o sistema de ultrafiltração com fluxo tangencial (SUFT). As SH foram caracterizadas por técnicas espectroscópicas na região do infravermelho e do ultravioleta-visível e por fluorescência molecular. Foram realizadas duas coletas de sedimentos, uma no período de chuva e outra no de seca, sendo coletados em três rios da Bacia Hidrográfica do Turvo/Grande, Rio Preto, Rio Turvo e Rio Grande. O sedimento foi coletado com o auxilio de uma draga manual do tipo Van Veen. As SH foram extraídas de acordo com metodologia oficial sugerida pela Sociedade Internacional de Substâncias Húmicas (SISH). A constante de estabilidade condicional (Kc) foi determinada por meio da supressão da fluorescência e variou de 5,6x10-3 – 1,23 em período chuvoso para o íons cobre, e de 5,5x10-3 – 8,7x10-2 para o período de seca. Quando a Kc foi determinada para o complexo SH-Cr(III) valores de 1,9x10-1-5,2x10-1 e 2,1x10-1-3,5x10-1, foram determinados para o período de chuva e de seca, respectivamente. Por fim a Kc foi determinada para a interação das SH com Cr(VI), de forma geral os valores de Kc foram maiores para o período de chuva. A capacidade complexante (CC) determinada por meio do SUFT para a interação das SH com cobre(II) teve valores que variaram de 4,0x10-1 – 6,3x10-1 mmol Cu g-1 SH e de 1,4x10-1 – 5,3x10-1 mmol Cu g-1 SH para período de chuva e seca, respectivamente. Para o Cr(III) a CC variou de 2,1x10-1 – 9,7x10-1 e de 2,6x10-1 – 8,4x10-1 mmol Cr g-1 SH para o período de chuva e seca, respectivamente. Quando a interação das SH com o Cr(VI) foi estudada por meio do SUFT só foi possível calcular a CC para os pontos...This work aimed to study the interactions of humic substances (HS) with copper(II), chromium(III) and chromium(VI), using by two methods, the suppression of the molecular fluorescence and tangential flow ultrafiltration sytem (TFUS). The HS were characterized by spectroscopic thecniques in the infrared and ultraviolet/visible region and molecular fluorescence. There were two sediment samples, one during the wet season and another the dry, being these sediments collected from the three Rivers of the Turvo/Grande drainage basin, Preto river, Turvo river and Grande river. The collection of sediment was carried out with the aid of a manual Van Veen type dredge. The HS were extracted according to the official methodology suggested by the International Humic Substances Society (IHSS). The conditional stability constant (Kc) was determined by suppressing fluorescence and ranged from 5,6x10-3 – 1,23 in the wet season for the copper ions, and of the 5,5x10-3 – 8,7x10-2 to the dry season. When Kc was determined for the complex HS-Cr(III) values of 1,9x10-1-5,2x10-1 e 2,1x10-1-3,5x10-1, were determined for the wet and dry seasons, respectively. Finally, the Kc was determined for the interactions oh HS with the Cr(VI) in geral the Kc values were higher for the wet season. Complexant capacity (CC) was determined by means of TFUS for the interactions of the HS with Copper(II) the values ranged from 4,0x10-1 – 6,3x10-1 mmol Cu g-1 HS and of 1,4x10-1 – 5,3x10-1 mmol Cu g-1 HS for a wet and dry season, respectively. For the Cr(III) CC ranged from 2,1x10-1 – 9,7x10-1 and from 2,6x10-1 – 8,4x10-1 mmol Cr g-1 HS for the wet and dry period, respectively. When the interactions oh HS with Cr(VI) was studied by means of TFUS was only possible to calculate the CC for the points NRP and CAPRP, as for others... (Complete abstract click electronic access below)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES
SU‐FF‐T‐340: A New Parameterisation of Small Photon Fields in Off‐Axis Regions for the 160MLC
No full text
An investigation into the nature and development of dance consciousness in choreography and performance
No full textThe dancer's consciousness is an integral component of choreography and performance, but has been afforded little significant attention within dance theory or practice. This thesis aims to investigate the nature of the dancer's consciousness and it develops a model which allows dancers to access particular conscious states in choreography and performance. This model is entitled the Dance Consciousness
Model.
The model is founded on consciousness theory and, within it, two states of dance consciousness have been defined: intrattentive consciousness and non-intrattentive consciousness. These are based on the states of consciousness described by consciousness researchers,
dance theorists and practitioners and theatre theorists.
The Dance Consciousness Model comprises methods for examining and accessing the dance consciousness. During the formulation of these methods a variety of theories and practices were explored: phenomenological reduction, the explicitation session, somatics, sport psychology, visual and verbal processing, attention studies, Buddhist Introspection and Bodyweather. Relevant components were extracted from each theory and practice and applied to the discipline of dance. This resulted in the development of a series of methods for examining and accessing states of
intrattention and non-intrattention. These methods were explored, by trained dancers, in three empirical projects which comprised exploratory workshops, choreographic
and rehearsal processes and performances.
The projects were recorded on video and the dancers were interviewed at regular intervals. The analysis of the information collected was combined with the consciousness
theory established at the start of the study and resulted in the formulation of a consciousness training programme
and the Dance Consciousness Model. The training precedes
the application of the model and comprises a series of
workshops in which dancers are taught to access, examine
and switch between states of intrattention and non-intrattention. The Dance Consciousness Model is believed to
be the first of its kind and is intended for application
by dancers in the choreography and performance of contemporary dance
Government and elementary education in Britain in the mid-nineteenth century
No full textThis thesis attempts to describe the growth of the central government’s involvement in elementary education, and the corresponding growth of the staffing and expenditure of the Education Department in Whitehall, in terms that have explanatory force. It goes from 1833 to the early 1860s, covering the 1840s and 1850s in most detail. The first chapter establishes a theoretical framework within which education can take its place beside other examples of government intervention. It reasserts the relevance of A.V. Dicey's analysis of the movements of opinion and the corresponding legislative trends, and concludes that in the mid-nineteenth century a description as far as possible in terms of demand factors is the appropriate one. The next two chapters describe the structure and growth of the systems of building grants and pupil-teacher grants; and the consequences for the staffing and expenditure of the Education Department. These are traced in detail, allowing an assessment of the Department's efficiency and the adequacy of the staff to the work, and how these changed over the period. Chapter 4 examines the evidence for Treasury restrictiveness of the Education Department's activities, and finds little, contrary to the assumptions of many accounts of the period. Chapter 5 traces the development of the views of the Newcastle Commission, and of Gladstone's interventions, and relates them to the Revised Code. These are together interpreted as a reassertion, ultimately unsuccessful, of an individualist approach to government intervention against the increasingly collectivist tendency of the system as it had become
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