73 research outputs found
Inflammatory pathway in eutopic endometrium of women with endometriosis
No full textInflammatory pathway in eutopic endometrium of women with endometriosis
Scillitani G MD, Neri M MD, Serviddio G, Cantatore S MTL, De Rosario F Obst, Scutiero G MD ,
Altomare E MD, Greco P MD, and Matteo M MD
Endometriosis is a complex disorder among women of reproductive age associated with chronic pelvic pain, debilitating dysmenorrhoea and infertility . These symptoms are thought to be the consequence of an inflammatory environment within the pelvis. Recently, a growing body of evidence indicates that abnormal inflammation is not only present in the pelvic endometriotic lesions but also in the eutopic endometrium of these patients leading to a proinflammatory environment unsuitable for implantation. Hence the aim of this study was to analyze by immunoblotting and by polymerase chain reaction the expression of inflammatory cytokines known to be involved in chronic endometritis (CE), in the eutopic endometrium of women affected by endometriosis.
MATERIALS AND METHODS
Endometrial samples were obtained from 12 patients 6 women affected by endometriosis and 6 controls.
Histological and immunoistochemical study: An immunohistochemical investigation of tissue samples was performed utilizing antibodies anti-TNF, anti-IL-1, anti-IL-6, anti-IL-8, anti-IL-10, anti-MCP-1, and CD 138.
Western blot (WB): Approximately 50 mg of endometrium tissue collected were prepared for Western blot. The image was analysed by Uvitec, which detects the chemiluminescent blots of proteins staining (TNF, IL-1, IL-6, IL-8, IL-10, MCP-1).
PCR analysis: In 6 out of 12 patients PCR analysis was also performed in order to confirm immunoistochemical and WB analysis
RESULTS
Histological and immunohistochemical study and Western blotting The examination of endometrium specimens revealed the negative reaction for the antibodies anti-IL-6 and IL-10 in patients and controls; a strong positive reaction for the antibodies anti-IL-1β, IL-8, MCP1, an intermediate positive reaction for the antibodies anti- TNF and CD138 in the endometriosic group. The control group revealed a negative reaction for all immunohistochemical dye. The immunohistochemical results were confirmed by Western blotting and real time PCR
CONCLUSIONS
Results demonstrated, in the eutopic endometrium from women with endometriosis, an increased expression of cytokines known to be involved in subclinical chronic endometritis. The presence of a chronic pro inflammatory endometrial environment could concur to explain the poor reproductive potential and the other symptoms associated to this complex disorder
Primary ovarian pregnancy and its management.
No full textPrimary ovarian ectopic pregnancy, i.e., the implantation of the gestational sac in the ovary, is one of the rarest forms of ectopic pregnancy. Its incidence after natural conception ranges from 1 in 2000 to 1 in 60 000 deliveries and accounts for 3% of all ectopic pregnancies. The diagnosis is intricate and based on surgical and histopathological observations. The management is, in spite of medical improvement, based on surgery. We present a case of a 10-wk ectopic ovarian pregnancy managed laparoscopically, and we describe, through a review of the literature, the specific symptomatology, diagnostic criteria, and treatment of this particular pathology
Modelling interdependencies among critical infrastructures at urban scale
No full textModern urban areas are transforming into sophisticated systems integrating both structures and infrastructures. This interconnection significantly increases the risk of disasters, which involves typical urban areas at different levels with structural, social, psychological and economic consequences. Therefore, improving emergency preparedness and mitigating possible disaster-induced losses of populous modern cities is becoming crucial.
Critical infrastructure, e.g. the transportation, electric and water networks, can be damaged due to inherent fragility with respect to the initiating external hazard (e.g. earthquake). Buildings collapse after strong earthquakes is the typical source of malfunctioning for connected critical infrastructures. In this work, the effect of debris after structural collapse or extensive damages on related networks is studied. A new formula to evaluate the debris affected area as function of the geometric characteristics of the masonry buildings is proposed. This strategy can be implemented in a virtual city model that is recognized useful for decision makers to quantify the performance of critical infrastructures following a disaster and to plan better resilience strategies in order to limit losses and downtime
Multibody simulation model and dynamic behavior's analysis of E.464 locomotive manufactured by Bombardier
No full textHaemostatic bandage for the control of haemostasis after stripping of endometriotic cysts.
No full textExploring simulation tools for urban seismic analysis and resilience assessment
No full textNowadays, the refined models of simulation to evaluate the seismic damage in an urban area are becoming of paramount interest for the scientific community. Regional seismic damage simulation can potentially provide valuable information that can facilitate decision making, enhance planning for disaster mitigation, and reduce human and economic losses. However, the application of refined models is limited because of their high computational cost and needs of highly experienced users. For these reasons, these approaches remain academic experiences. This study proposes a straightforward approach to the problem, at the same time competitive, to simulate the seismic response and to assess the degree of damage at urban scale. At first, the simulation of the standard building is performed using an equivalent single degree of freedom model. Subsequently, the same approach is extended to a number of regular buildings from a virtual city sample for time-history seismic response analysis. The first part of this work is devoted to present the methodology to prepare the one-degree-of-freedom model of the standard building by comparing it with a refined multi degrees of freedom model as a target. Finally, a seismic damage simulation of a virtual city sample is implemented to demonstrate the capacity and advantages of the proposed method at increasing seismic intensities for damage assessment. It is the starting phase for further multi-hazards analyses at the regional scale through agent-based models
Benign metastasizing leiomyoma of external iliac vessels: an unusual case report
No full textNo abstract availabl
Cervical pregnancy treated by uterine artery embolisation combined with office hysteroscopy.
No full textOBJECTIVE: To evaluate the safety and efficacy of uterine artery embolisation in conjunction with hysteroscopic resection of trophoblast in the conservative treatment of cervical ectopic pregnancies. STUDY DESIGN: Five women diagnosed with cervical pregnancies at University Hospital of Foggia, Italy, between May 2009 and February 2012 underwent uterine artery embolisation followed by office hysteroscopic resection of trophoblast. Data on operating time, blood loss, blood transfusion, conversion to other techniques, complications related to surgery, change of serum β-hCG level, hospitalisation days and outcome of the women after discharge were collected. RESULTS: The mean hysteroscopic operative time was 9.8min, and the blood loss was negligible in all cases. Blood transfusion was not needed for any of the women. None of the women required conversion to other techniques. In all cases the operations were uneventful. The serum β-hCG level in all the cases declined to normal within 15 days of surgery. The total hospitalisation time was 4 days in all the cases. No vaginal bleeding or other side effects were observed throughout and after the treatment, all women recovered without complications. CONCLUSIONS: Uterine artery embolisation with office hysteroscopic resection is an effective option in treatment of cervical ectopic pregnancy
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