155 research outputs found

    Euplotes uncinatus (Ciliophora, Hypotrichia), a new species with zooxantellae

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    During the observations of a field collection of Maristentor dinoferus with fluorescence microscopy, we discovered a smaller ciliate with zooxanthellae. On the basis of morphological, morphometric and ultrastructural characters, this ciliate has been recognized as a new species of the genus Euplotes. To emphasize the peculiar shape and extension of the right buccal margin, the species has been named Euplotes uncinatus sp. n. The most distinctive traits of E. uncinatus are the presence of mucocyst-like vesicles, a kind of extrusome not previously found in Euplotes, and the presence of zooxanthellae. Evidence from ciliate behavior and zooxanthellae ultrastructure make it very likely that the zooxanthellae are symbiotic, even though some are evidently consumed by the host

    Euplotes uncinatus (Ciliophora, Hypotrichia), a new species with zooxanthellae

    No full text
    During the observations of a field collection of Maristentor dinoferus with fluorescence microscopy, we discovered a smaller ciliate with zooxanthellae. On the basis of morphological, morphometric and ultrastructural characters, this ciliate has been recognized as a new species of the genus Euplotes. To emphasize the peculiar shape and extension of the right buccal margin, the species has been named Euplotes uncinatus sp. n. The most distinctive traits of E. uncinatus are the presence of mucocyst-like vesicles, a kind of extrusome not previously found in Euplotes, and the presence of zooxanthellae. Evidence from ciliate behavior and zooxanthellae ultrastructure make it very likely that the zooxanthellae are symbiotic, even though some are evidently consumed by the host. © Springer-Verlag 2005

    5-Fluorouracil-treated planarians, a versatile model system for studying stem cell heterogeneity and tissue aging

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    Background Information: Planarians are a sound, well-established model system for molecular studies in the field of stem cells, cell differentiation, developmental biology and translational research. Treated stem cell-less planarians produced by X-ray treatment are commonly used to study stem cell transcriptional profile and their role in planarian biological processes. X-ray induces oxidative and DNA damage to differentiated cells, requires expensive radiation machines that are not available in most of the research centres and demand rigorous risk management and dedicated staff. Results: We tested the use of the well-known antimetabolite genotoxic drug 5-fluorouracil which mainly affects proliferating cells in way to demonstrate its use in replacing X-ray treatment. We succeeded in demonstrating ability of high doses of 5-fluorouracil to deplete Dugesia japonica stem cells and in identifying a 5-fluorouracil transiently resistant population of lineage committed stem cells. Conclusions and Significance: Our results encourage the use of 5-fluorouracil-treated planarians as a model system for studying mechanisms of resistance to genotoxicants, planarian stem cell heterogeneity and molecular cascades of tissue aging
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