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    ESTROUS AND FERTILITY RESPONSES OF DAIRY EWES SYNCHRONIZED WITH COMBINED SHORT TERM GnRH, PGF2α AND ESTRADIOL BENZOATE TREATMENTS

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    The objective of this study was to asses the effectiveness of short term protocols (5–7 days) for the synchronization of oestrus in lactating ewes during the non-breeding season, using combinations of GnRH, PGF2 and estradiol benzoate treatments. One hundred and six adult cross-bred Altamurana ewes were divided into the following six treatment groups: (i) Group NT (N= 30), which served as the control, was exposed to fertile rams for a 20 days period; (ii) Group Fe (N= 15) received FGA intravaginal sponges (14 days) + eCG i.m. (Day 14); (iii) Group GP (N= 15) received a GnRH agonist (Day 0) and PGF2 (Day 5) i.m. treatment; (iv) Group GPG (N= 15) received a GnRH (Day 0), PGF2 (Day 5) and a second dose of GnRH (Day 7) i.m.; (v) Group GPE ewes (N= 16) treated with GnRH (Day 0), PGF2 (Day 5) and estradiol benzoate (EB; Day 6, 24 h after PGF2) i.m. treatment; (vi) Group EPE (N= 15) treated with EB (Day 0), PGF2 (Day 5) and EB (Day 6, 24 h after PGF2) i.m. treatment. Ewes were checked for oestrus and hand mated using fertile rams. Upon return to service the ewes were mated again. A definite effect of the treatments on the occurrence of oestrus was recorded, with a higher percentage of ewes (P < 0.01) exhibiting oestrus in the Fe (93.3%) and EPE (100%) groups, compared to the GPG (33.3%), GP (46.7%) and GPE (62.5%) groups. The interval to oestrus was shorter and more synchronized in the long term treated Fe group (P < 0.01). The fertility rates recorded were 36.7% (11/30) in the NT or control ewes, and 60.0% (9/15), 33.3% (5/15) and 26.7% (4/15) (not significant) in the Fe, GP and GPG groups, respectively. In the GPG group the fertility and prolificacy rates recorded were higher (80% and 175%), than in any of the other treatment groups. In the GPE group, 10 of the 16 ewes (62.5%) exhibited oestrus, but the fertility rate was significantly lower (20%, P < 0.01), compared to the other groups (GPG, 80%; GP, 71.4%; Fe, 64.3%). In Group EPE, despite having the highest percentage of ewes exhibiting oestrus (100%), none of the ewes lambed. The fertility achieved in all the short term treatment groups following natural service and oestrous synchronization indicate that the protocols used do not seem to interfere with the ewes natural cycle or with their fertility. Results indicate that between the short term treatments studied, the GP and GPG methods offer a potential alternative to the conventional long term protocol (Fe) for oestrous synchronization in dairy ewes. Further research is required regarding the use of estradiol benzoate to synchronize oestrus

    How the FSH/LH ratio and dose numbers in the p-FSH administration treatment regimen and insemination schedule affect superovulatory response in ewes

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    We wished to evaluate the effects of FSH/LH ratio and number of doses of p-FSH during a superovulatory treatment on ovulation rate and embryo production (Experiment I). In Experiment II, we studied the efficacy of fertilization after various insemination schedules in superovulated donors. In Experiment I estrus was synchronized in 40 ewes (FGA, for 9 days plus PGF2 alpha on Day 7) and the ewes were randomly assigned to four treatment groups as follows (n = 10 ewes each): Group A: four p-FSH doses with the FSW/LH ratio held constant (1.6); Group B: four p-FSH doses with the FSH/LH ratio decreasing (FSH/LH 1.6-1.0-0.6-0.3); Group C: eight p-FSH doses with the FSH/LH ratio held constant (1.6); Group D: eight p-FSH doses and FSH/LH ratio decreasing (1.6-1.6, 1.0-1.0, 0.6-0.6, 0.3-0.3). p-FSH administrations were performed twice daily 12 h apart. The ewes were mated at the onset of estrus and again after 12 and 24 h; then, one rain per four ewes was maintained with the ewes for two additional days. Ovarian response and embryo production were assessed on Day 7 after estrus. Experiment II. Three groups (n = 10 each) of superovulated ewes were inseminated as follows: Group M: mated at onset of estrus; Group AI: artificial insemination 30 h after onset of estrus; M + AI) mating at onset of estrus and intrauterine AI performed 30 h from estrus with fresh semen. Results of Experiment I showed that treatment (D) improved (P < 0.05) ovulatory response in comparison to Groups (C) and (A). The fertilization rate was lower (P < 0.01) in Group D) than Group (A). Also the proportion of transferable embryos was lower in Group (D) in comparison to all the other treatments (P < 0.01). Group A gave the best production of embryos (7.3/ewe; 89.0% transferable). In Experiment II, combined mating plus At improved fertilization rate (80.3%) compared to both mating (P < 0.01) and At (P < 0.02) alone
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