4,236 research outputs found
"A veritable Augustus": the life of John Winthrop Hackett, newspaper proprietor, politician and philanthropist (1848-1916)
No full textIrish-born Sir John Winthrop Hackett (1848-1916) achieved substantial political and social standing in Western Australia through his editorship and part-ownership of the West Australian newspaper, his position as a Legislative Council member and as a layman in the Anglican Church. The thesis illustrates his strong commitment to numerous undertakings, including his major role in the establishment of Western Australia's first University.
This thesis will argue that whatever Hackett attempted to achieve in Western Australia, his philosophy can be attributed to his Irish Protestant background including his student days at Trinity College Dublin. After arriving in Australia in 1875 and teaching at Trinity College Melbourne until 1882, his ambitions took him to Western Australia where he aspired to be accepted and recognised by the local establishment. He was determined that his achievements would not only be acknowledged by his contemporaries, but also just as importantly be remembered in posterity. After a failed attempt to run a sheep station, he found success as part-owner and editor of the West Australian newspaper.
Outside of his business interests, Hackett’s commitment to the Anglican Church was unflagging. At the same time, he was instrumental in bringing about the abolition of state aid to church schools in Western Australia, which he saw as advantaging the Roman Catholic Church. He was a Legislative Council member for 25 years during which time he used his editorship of the West Australian, to campaign successfully on a number of social, industrial and economic issues ranging from divorce reform to the provision of economic infrastructure. As a delegate to the National Australasian Conventions he continually strove to improve the conditions under which Western Australia would join Federation. His crowning achievement was to establish the state’s first university, which he also generously provided for in his will. One of the most influential men in Western Australian history, his career epitomised the energy and ambition of the well-educated immigrant
A trinity man abroad: Sir Winthrop Hackett
No full textThere was a time when Western Australians remembered the goldrush decade of the 1890s as ruled by a triumvirate of Sir John Forrest, Winthrop Hackett, and Bishop Charles Riley. As one elderly man put it to me: 'Forrest ran the politics, Hackett ran the press, and Riley was minister for junket'. This was never quite the whole story, as Riley did not arrive until 1894, and Forrest had other powerful advisers such as his brother Alexander and Charles Harper; but it reflected the power that influential individuals in strategic roles could exercise in a small community, as well perhaps as the Masonic links between Hackett and Riley. None of the triumvirate has an adequate biography although as I write the publication of Frank Crowley's magnum opus on Forrest is imminent. Good short accounts have been written for the Australian Dictionary of Biography by Lyall Hunt on Hackett and Peter Boyce on Riley, but the bigger studies have yet to come
Student Teaching Internship, Monsignor John R. Hackett High School
No full text6 p.The author describes her experience as a high school student teacher.Monsignor John R. Hackett High School. Kalamazoo, Michigan
14th annual John Perkins Lecture
No full textDr. John Perkins returns to campus Tuesday, April 30, 11 a.m., at First Free Methodist Church, for the 14th annual John Perkins Lecture Series. This year’s morning event features Erna Kim Hackett, executive pastor at The Way Berkeley. Later that day, Hackett and Dr. Perkins will continue the morning’s topic, “Words Have Power,” at 7:30 p.m., also in First Free Methodist Church.
John Perkins is one of the leading evangelical voices to come out of the American civil rights movement and an internationally known author, speaker, and teacher. His is the co-founder of SPU’s John Perkins Center for Reconciliation, Leadership Training, and Community Development and the author of the new book One Blood: Parting Words to the Church on Race and Love. Erna Kim Hackett served with InterVarsity for 18 years in Black Ministries and Urban Programs. She is a preacher, pastor, writer, activist, and singer
MCL-1 localizes to sites of DNA damage and regulates DNA damage response
No full textMCL-1, a pro-survival member of the BCL-2 family, was previously shown to have functions in ATR-dependent Chk1 phosphorylation following DNA damage. To further delineate these functions, we explored possible differences in DNA damage response caused by lack of MCL-1 in mouse embryo fibroblasts (MEFs). As expected, Mcl-1(-/-) MEFs had delayed Chk1 phosphorylation following etoposide treatment, compared to wild type MEFs. However, their response to hydroxyurea, which causes a G(1)/S checkpoint response, was not significantly different. In addition, appearance of gamma-H2AX was delayed in the Mcl-1(-/-) MEFs treated with etoposide. We next investigated whether MCL-1 is present, together with other DNA damage response proteins, at the sites of DNA damage. Immunoprecipitation of etoposide-treated extracts with anti-MCL-1 antibody showed association of MCL-1 with gamma-H2AX as well as NBS1. Immunofluorescent staining for MCL-1 further showed increased co-staining of MCL-1 and NBS1 following DNA damage. By using a system that creates DNA double strand breaks at specific sites in the genome, we demonstrated that MCL-1 is recruited directly adjacent to the sites of damage. Finally, in a direct demonstration of the importance of MCL-1 in allowing proper repair of DNA damage, we found that treatment for two brief exposures to etoposide , followed by periods of recovery, which mimics the clinical situation of etoposide use, resulted in greater accumulation of chromosomal abnormalities in the MEFs that lacked MCL-1. Together, these data indicate an important role for MCL-1 in coordinating DNA damage mediated checkpoint response, and have broad implications for the importance of MCL-1 in maintenance of genome integrity.Peer reviewedfinal article publishedprotein complexchromosomesG2/McheckpointDNA repai
2007 - biennial review - 2009
No full textReporting period: July 1, 2007-June 30, 2009.Assembled and reviewed by: Reagan Waskom, Nancy Grice, Zach Hittle, Kevin Hackett
Corrigendum to “Accumulation and potential for transport of microplastics in stormwater drains into marine environments, Perth region, Western Australia” [Mar. Pollut. Bull. 168 (2021) 112362]
No full textThe authors regret that the original manuscript failed to appropriately acknowledge all sources of intellectual and technical contribution, which warrant co-authorship. Dr Mark J Hackett (School of Molecular and Life Sciences, Curtin University, Bentley 6102 WA, Australia) is now recognised as a co-author of this manuscript with respect to intellectual input into the design of ATR-FTIR experiments and interpretation of the ATR-FTIR spectra. Dr Hackett is also recognised as the sole co-author responsible for training the first author of this paper in the use of ATR-FTIR research equipment. The ATR-FTIR equipment used for this study is housed within the School of Molecular and Life Sciences at Curtin University, and all authors gratefully acknowledge Curtin University for providing access to this equipment. The authors would like to apologise for any inconvenience caused. Natasha Lutz: Conceptualization, Methodology, Software, Data curation, Investigation, Formal analysis, Visualization, Writing – original draft, Writing – review & editing. James Fogarty: Data curation, Formal analysis, Methodology, Writing – review & editing, Supervision. Andrew Rate: Conceptualization, Methodology, Writing – review & editing, Supervision. Mark J. Hackett: Investigation, Data curation, Visualization
Facet Theory and the Mapping Sentence: Evolving Philosophy, Use and Application
No full textHow do we think about the worlds we live in? The formation of categories of events and objects seems to be a fundamental orientation procedure. Facet theory and its main tool, the mapping sentence, deal with categories of behavior and experience, their interrelationship, and their unification as our worldviews. In this book Hackett reviews philosophical writing along with neuroscientific research and information form other disciplines to provide a context for facet theory and the qualitative developments in this approach. With a variety of examples, the author proposes mapping sentences as a new way of understanding and defining complex behavior
Oxidative modification of albumin in the parenchymal lung tissue of current smokers with chronic obstructive pulmonary disease
No full textBackground: there is accumulating evidence that oxidative stress plays an important role in the pathophysiology of chronic obstructive pulmonary disease (COPD). One current hypothesis is that the increased oxidant burden in these patients is not adequately counterbalanced by the lung antioxidant systems.Objective: to determine the levels of oxidised human serum albumin (HSA) in COPD lung explants and the effect of oxidation on HSA degradation using an ex vivo lung explant model.Methods: parenchymal lung tissue was obtained from 38 patients (15F/23M) undergoing lung resection and stratified by smoking history and disease using the GOLD guidelines and the lower limit of normal for FEV1/FVC ratio. Lung tissue was homogenised and analysed by ELISA for total levels of HSA and carbonylated HSA. To determine oxidised HSA degradation lung tissue explants were incubated with either 200 ?g/ml HSA or oxidised HSA and supernatants collected at 1, 2, 4, 6, and 24 h and analysed for HSA using ELISA and immunoblot.Results: when stratified by disease, lung tissue from GOLD II (median = 38.2 ?g/ml) and GOLD I (median = 48.4 ?g/ml) patients had lower levels of HSA compared to patients with normal lung function (median = 71.9 ?g/ml, P < 0.05). In addition the number of carbonyl residues, which is a measure of oxidation was elevated in GOLD I and II tissue compared to individuals with normal lung function (P < 0.05). When analysing smoking status current smokers had lower levels of HSA (median = 43.3 ?g/ml, P < 0.05) compared to ex smokers (median = 71.9 ?g/ml) and non-smokers (median = 71.2 ?g/ml) and significantly greater number of carbonyl residues per HSA molecule (P < 0.05). When incubated with either HSA or oxidised HSA lung tissue explants rapidly degraded the oxidised HSA but not unmodified HSA (P < 0.05).Conclusion: we report on a reliable methodology for measuring levels of oxidised HSA in human lung tissue and cell culture supernatant. We propose that differences in the levels of oxidised HSA within lung tissue from COPD patients and current smokers provides further evidence for an oxidant/antioxidant imbalance and has important biological implications for the disease.<br/
A molecular timeline for the origin of photosynthetic eukaryotes
No full textThe appearance of photosynthetic eukaryotes (algae and plants) dramatically altered the Earth's ecosystem, making possible all vertebrate life on land, including humans. Dating algal origin is, however, frustrated by a meager fossil record. We generated a plastid multi-gene phylogeny with Bayesian inference and then used maximum likelihood molecular clock methods to estimate algal divergence times. The plastid tree was used as a surrogate for algal host evolution because of recent phylogenetic evidence supporting the vertical ancestry of the plastid in the red, green, and glaucophyte algae. Nodes in the plastid tree were constrained with six reliable fossil dates and a maximum age of 3,500 MYA based on the earliest known eubacterial fossil. Our analyses support an ancient (late Paleoproterozoic) origin of photosynthetic eukaryotes with the primary endosymbiosis that gave rise to the first alga having occurred after the split of the Plantae (i.e., red, green, and glaucophyte algae plus land plants) from the opisthokonts sometime before 1,558 MYA. The split of the red and green algae is calculated to have occurred about 1,500 MYA, and the putative single red algal secondary endosymbiosis that gave rise to the plastid in the cryptophyte, haptophyte, and stramenopile algae (chromists) occurred about 1,300 MYA. These dates, which are consistent with fossil evidence for putative marine algae (i.e., acritarchs) from the early Mesoproterozoic (1,500 MYA) and with a major eukaryotic diversification in the very late Mesoproterozoic and Neoproterozoic, provide a molecular timeline for understanding algal evolution
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