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Simultaneous seminoma and interstitial cell tumour in a rabbit with a previous cutaneous basal cell tumor
No full textThe development of spontaneous multiple tumours is a rare event in domestic rabbits. The diagnosis of a cutaneous basal cell tumour and the successive development of simultaneous bilateral testicular tumours with dissimilar histology (a seminoma and an interstitial cell tumour) are described in a vasectomized, crossbred dwarf rabbit, aged 6 years. Two cases of basalioma associated with uterine adenocarcinoma have been previously described in rabbits. A similar association between basal cell neoplasia and development of tumours (e.g., testicular and breast cancer) at cutaneous and non-cutaneous sites has been reported in man
Diagnostico de peritonitis infecciosa felina (PIF) mediante tecnicas de biopsia no invasivas
No full textL'intervento descrve i risutlati di uno studio sulla possibilità di diagnosticare la peritonite infettiva felina (FIP) mediante prelievi bioptici, evidenziando una buona specificità ma una limitata sensibilità diagnostic
Injection site reaction (Pseudolymphoma) mimiking a primary cutaneous lymphoma in a cat
No full textA 4-years old, spayed female, domestic shorthaired cat was presented for a slow growing, non painful, not ulcerated, 1 cm, subcutaneous mass in the dorso-lateral thorax in the site of a previous drug injection (Ranitidine). The cat was in a good body condition, CBC and serum biochemistry were unremarkable. Fine-needle aspirate cytological samples were interpreted by the referring veterinarian as cutaneous lymphoma. Stained and unstained samples were sent for consult. Cytology was characterized by a prevalence of medium sized lymphocytes with round to indented nuclei, finely granular to diffuse chromatin (centrocytes) admixed with small mature lymphocytes, occasional centroblasts and macrophages. Findings were consistent with a chronic lymphohistiocytic dermatitis. To definitely rule out a cutaneous lymphoma the lesion was surgically excised. Histopathology revealed a subcutaneous oval, well demarcated, unencapsulated mass consisting of a mixed inflammatory and reparative reaction containing plump reactive fibroblasts, plasmacells, histiocytes, eosinophils and, occasional mast cells associated with small mature and medium sized reactive lymphocytes either infiltrating the nodule or organized at the periphery in small pseudofollicular and perivascular aggregates.
COMMENT: The lesion resembled the so-called vaccine induced cutaneous pseudolymphoma described in man that is characterized by a reactive B cell proliferation associated with abundant histiocytes often in clusters. Pseudolymphoma is a term applied to a group of inflammatory to proliferative lesions that may be misdiagnosed as lymphoma as was in this case by cytology. In cats, arthropod bites and vaccination are probably the main triggers of pseudolymphoma. These lesions are mostly polyclonal (B or T) benign, self-limiting reactions induced by persistent antigenic stimulation. However, in some cases pseudolymphoma may evolve into cutaneous lymphoma
Prostatic sarcomatoid carcinoma in a dog : cytological and immunohistochemical findings
No full textAn 8-year-old neutered male Boxer was presented with tenesmus, hemorrhagic urethral discharge, and dysuria. Abdominal ultrasound and radiographic examinations revealed irregular prostatic enlargement. Laparotomy was performed and intraoperative cytology was done on imprint smears of a biopsy specimen obtained from a prostatic mass. The cytologic preparation was highly cellular and contained a predominant population of atypical, large, loosely cohesive spindle cells, with rare multinucleated cells and mitotic figures. The cytologic findings were consistent with undifferentiated sarcoma. At necropsy, a large cystic prostatic mass and numerous satellite nodules in the soft tissues around the pelvis were found. On histologic examination the tumor was composed primarily of bundles of neoplastic spindle cells. Rare pseudo-acinar structures and signet-ring cells also were observed. On immunohistochemical examination, the neoplastic cells co-expressed cytokeratin and vimentin. Based on histologic and immunohistochemical findings, the tumor was diagnosed as primary prostatic sarcomatoid carcinoma. This is a rare tumor in dogs, in which biphasic morphology of epithelial and mesenchymal cells can complicate the diagnosis, requiring immunochemical stains for confirmation
Primary angiocentric/angioinvasive T-cell lymphoma of the tympanic bulla in a FeLV positive cat
No full textIntroduction: Reports of primary middle ear lymphoma are rare in
cats and their extension to internal organs is exceptional. A 5-yearold,
neutered female, FeLV-positive domestic shorthair cat was
referred for stertor, dyspnoea and head-tilt. CT scan revealed soft tissue
opacity inside the right tympanic bulla with bone lysis and concurrent
nasopharyngeal and intracranial invasion.
Materials and Methods: Endoscopic-guided biopsy samples were
collected for histology and immunohistochemistry. A full necropsy examination
was performed.
Results: Grossly, the lesion was poorly demarcated, white and soft.
Cytology identified round, large, plasmacytoid neoplastic cells. Histology
revealed dense sheets of round neoplastic cells often surrounding
or invading vascular walls (angiocentric/angiodestructive
pattern). Neoplastic cells expressed CD3 (T cell phenotype) and
FeLV p27 and gp70 antigens. A middle ear angiocentric angioinvasive
T-cell lymphoma was diagnosed. Following radiation therapy,
clinical conditions improved, but dysphagia recurred and the cat
died suddenly. At necropsy examination, a soft, red mass filled the
ventromedial compartment of the tympanic bulla. Extension to the
base of the skull with right piriform lobe compression was recorded.
Hepatic and splenic metastases were present.
Conclusions: Diagnosis of primary middle ear tumors is often delayed
since clinical signs mimic more common otological conditions.
Multiple biopsy specimens and immunohistochemistry were pivotal
for the diagnosis in this case. FeLV might have been involved in tympanic
lymphoma development
Leucemia eritroide acuta in un cane
No full textUn cane meticcio maschio di 10 anni con grave pancitopenia viene sottoposto a emotrasfusione e terapia con prednisone. Anemia e trombocitopenia peggiorano progressivamente. Dopo 5 giorni compaiono alcune cellule atipiche di aspetto immaturo nello striscio ematico. All’esame citologico del midollo osseo si riscontra una netta prevalenza di cellule di grandi dimensioni con elevato rapporto N/C, citoplasma intensamente blu e nucleo rotondo/ovale con cromatina finemente granulare e nucleoli evidenti (compatibili con rubriblasti/prorubriciti con spiccata diseritropoiesi). Tali cellule sono talora binucleate. Immunocitochimica e citofluorimetria confermano l’origine eritroide, in quanto queste cellule risultano positive per CD44 (cellule eritroidi e mieloidi) e negative per tutti i marker pan-leucocitari (CD45 e CD18), e di differenziazione mieloide e linfoide, portando a una diagnosi di leucemia eritroide acuta (AML-M6). Il cane viene sottoposto a eutanasia dopo 38 giorni per peggioramento delle condizioni cliniche.
La AML-M6 è una patologia rara anche in medicina umana. Nell’uomo è generalmente associata ad anemia, trombocitopenia e neutropenia, mentre più raramente si riscontrano cellule neoplastiche in circolo. Secondo la classificazione WHO del 2008, la diagnosi viene fatta quando nel midollo osseo più del 50% delle cellule sono precursori eritroidi con marcata diseritropoiesi e il 20% delle cellule non eritroidi sono blasti.
Seppur non infrequente nel gatto, quello descritto è il secondo caso di AML-M6 spontanea segnalato nel cane. Il quadro clinico-patologico è sovrapponibile a quello dell’uomo. Tuttavia, nell’unico altro caso segnalato nel cane numerose cellule neoplastiche erano presenti in circolo già al momento della diagnosi, evento che si riscontra in meno della metà dei casi in medicina umana
Qualitative evaluation of tortellini meat filling by histology and image analysis
No full textThe aim of this work was to identify the presence of different animal tissues in tortellini meat-filling with special emphasis on the percentage of skeletal muscle. Industrially packed and refrigerated tortellini filling from 4 different Italian commercial brands were examined and compared. From each brand, 5 different batches were sampled at intervals of one month. From each batch, 4 fillings were randomly sampled and coded to allow a blind examination. Histological sections were examined to identify the presence of various animal tissues. The presence and amount of large fragments of skeletal muscle in specimens were assessed and semiquantitatively ranked. To evaluate the percentage area of skeletal muscle in the samples, a computerized image analysis system was used. Animal tissues were well preserved in all examined samples. The filling quality was quantified by histology and image analysis evaluating: skeletal muscle density, size of skeletal muscle fragments, and uniformity of batches. In addition to the effective enforcement of food labelling law and the protection of consumers, histology and image analysis are reliable tools to identify small amounts of various animal tissues in a processed meat product
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