729 research outputs found
USE OF AUTOLOGOUS PLATELET GEL FOR RECURRENCE OF PERINEAL AND SACROCOCCIGEAL FISTULA
No full textUSE OF AUTOLOGOUS PLATELET GEL FOR RECURRENCE OF PERIANAL AND SACRO-COCCIGEAL FISTULA A. Giuliani, G. Ferrazza, D. Crocetti, L. de Anna, E. Orsi, F. Guerra, F. La Torre Coloproctology Unit, Department of Emergency, Policlinico Umberto I, University of ‘‘La Sapienza’’, Rome, Italy Abstract The treatment of Sinus Pilonidalis and Perianal Fistulas is still not entirely standardised due to the frequency of relapses, the inconvenience for patients and last but not least, for the economic commitment by the health-care structure. The potential therapeutic value and versatility of products stemming from blood and platelets have long stimulated research and interest in the field of surgery. The autologous gel platelets (PG), generated by activated human thrombin and platelets, represent a new biotechnology for angiogenesis stimulation on speeding up the healing and regeneration of tissues. Do not underestimate its effect as analgesic. Our study shows the employment of gel platelets in the treatment of complicated wounds and recurrences following surgery for Sinus Pilonidalis and Perianal Fistulas . From January 2006 to May 2009, we selected eight patients (6 males and 2 females, aged between 19 and 54 years) with infected wound following surgery for Sinus Pilonidalis and 10 patients (6 males and 4 females, aged between 31 and 72 years) with recurrence after Perianal Fistulas Our results, although obtained on a small group of patients, support the safety and efficacy of PG in the treatment of infected wounds following surgery for the excision of Sinus Pilonidalis and Perianal Fistulas relapses. Further studies and longer follow-up are needed to come to any conclusion
Il gel piastrinico nel trattamento del “piede diabetico”: esperienza preliminare
No full textIL GEL PIASTRINICO NEL TRATTAMENTO DEL “PIEDE DIABETICO”: ESPERIENZA PRELIMINARE
Massimo Chiaretti*, Giancarlo Ferrazza+, Rita Maria Fracassi@ , Andrea Negro°, Domenico Tuscano*
(*) Dipartimento di Chirurgia Generale, Specialità Chirurgiche e Trapianti d’Organo “Paride Stefanini” Azienda Policlinico Umberto I, Università degli Studi di Roma “La Sapienza”
(+ ) Servizio di Immunoematologia e Medicina Trasfusionale Azienda Policlinico Umberto I, Università degli Studi di Roma “La Sapienza”
(@) Servizio di Immunoematologia IRCCS Ospedale Bambino Gesù, sede di Roma.
(°) Azienda S. Andrea, II Facoltà di Medicina e Chirurgia dell’Università degli Studi di Roma “La Sapienza”
La nostra esperienza evidenzia l’utilità del gel piastrinico (GP) nel trattamento ambulatoriale delle piaghe torpide della pianta del piede in paziente diabetico. Il GP guarisce in tempi prevedibili ulcerazioni torpide che richiedevano lunghissimi periodi di medicazioni complesse senza raggiungere il risultato prefissato. La nostra esperienza, analogamente ai risultati di altri autori, depone favorevolmente per questo trattamento. La metodica personalmente messa a punto prevede il curettage dopo disinfezione, l’inoculazione di 1 UI di insulina pronta alla base del cratere ulcerativo e quindi l’applicazione di 5 ml di GP con 1 ml di Trombina autologa attivata. L’applicazione può essere eseguita mediante un puntale spray o cannula a seconda delle esigenze dell’operatore o del tipo di lesione. In caso di ferite più piccole o man mano che la ferita in trattamento si riduce di ampiezza, il prodotto può essere aliquotato e congelato per successive somministrazioni. Il GP e la trombina vanno spalmati in situ e su un supporto inerte riassorbibile di acido ialuronico (Hialogel) con il quale si zaffa la cavità e che impedisce la dispersione della parte liquida e non completamente gelificabile, ricca dei fattori di crescita (PDGF, TGF, EGF, ed altri). Il rapporto strettamente collaborativo tra dermatologo, chirurgo, ortopedico, immunotrasfusionista, diabetologo e cardiologo, risolve questa problematica con un approccio multidisciplinare. Lo stretto coordinamento tra centro trasfusionale ed ambulatorio chirurgico evita inutili attese al Paziente, accorcia i tempi di esecuzione della medicazione, abbatte i costi per l’impiego di materiali e alla lunga diventa conveniente rispetto al tradizionale trattamento.Our study proves the usefulness of platelet gel in the treatment of the diabetic foot. We started in January 2006 to treat diabetic wounds of the foot in the outpatients’ surgical department with encouraging results. Despite its expensive and complex preparation, the platelet gel is useful and convenient because it succeeds in shortening the ambulatory treatment period. Besides, in our opinion, the multidisciplinary approach of this treatment is rather important: actually, it implies the cooperation of dermatologist, surgeon, orthopaedist, immunologist, diabetologist and, if necessary, the cardiologist. That is why it reduces wastes of work-time and the expenses for consultants, medications and dressing material
First case of bronchiolar-pleural fistula repair with platelet-leukocyte rich gel
No full textBronchiolar-pleural fistulas are a
frequent complication of thoracic surgery. Current
treatment strategies and their invasiveness
are quiet different, but often surgeons decide for
a new surgical intervention and definitive closure
of the breach. We report the case of a bronchiolar-
pleural fistula in a 75 years old man with
important co-morbidities that we treated with instillation
of platelet-leukocyte rich gel (PLR-G).
We disuss actual indications for PLR-G as well
as its possible role in thoracic surgery
CD34+ cell mobilization for allogeneic progenitor cell transplantation: efficacy of a short course of G-CSF
No full textG-CSF-mobilized PBPCs are considered the richest source of HPCs for both autologous and allogeneic transplantation, but, despite their wide use, the best dose and schedule for G-CSF administration have not been definitively established
Cardiopulmonary exercise testing in the functional and prognostic evaluation of patients with pulmonary diseases
No full textExercise testing is increasingly utilized to evaluate the level of exercise intolerance in patients with lung and heart diseases. Cardiopulmonary exercise testing (CPET) is considered the gold standard to study a patient's level of exercise limitation and its causes. The 2 CPET protocols most frequently used in the clinical setting are the maximal incremental and the constant work rate tests. The aim of this review is to focus on the main respiratory diseases for which exercise tolerance is indicated; for example, chronic obstructive pulmonary disease, interstitial lung disease, primary pulmonary hypertension and cystic fibrosis. This review also focuses on the variables/indices that are utilized in the functional and prognostic evaluation. The recognition of abnormal response patterns of ventilatory, cardiac and metabolic limitation to exercise may help in the diagnostic evaluation. In addition, CPET indexes can provide important functional and prognostic information regarding patients with pulmonary disease. Exercise indices, such as peak oxygen uptake (V'O 2 peak), ventilatory equivalents for carbon dioxide production (V'E-/V'CO2) and arterial oxygen saturation (S pO2), have in fact proven to be better predictors of prognosis than lung function measurements obtained at rest. Moreover, useful information on the effects of therapeutic interventions may be obtained by CPET by studying the changes in endurance capacity during high-intensity constant work rate protocols. Copyright © 2009 S. Karger AG
IsotopicLabelling: an R package for the analysis of MS isotopic patterns of labelled analytes
No full textMotivation: Labelling experiments in biology usually make use of isotopically-enriched substrates, with the two most commonly employed isotopes for metabolism being 2 H and 13 C. At the end of the experiment some metabolites will have incorporated the labelling isotope, to a degree that depends on the metabolic turnover. In order to propose a meaningful biological interpretation, it is necessary to estimate the amount of labelling, and one possible route is to exploit the fact that MS isotopic patterns reflect the isotopic distributions.
Results: We developed the IsotopicLabelling R package, a tool able to extract and analyse isotopic patterns from liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-MS (GC-MS) data relative to labelling experiments. This package estimates the isotopic abundance of the employed stable isotope (either 2 H or 13 C) within a specified list of analytes.
Availability: The IsotopicLabelling R package is freely available at https://github.com/RuggeroFerrazza/IsotopicLabellin
TLC surface integrity affects the detection of alkali adduct ions in TLC-MALDI analysis
No full textDirect coupling of thin-layer chromatography (TLC) with matrix-assisted laser desorption ionization (MALDI) mass spectrometry allows fast and detailed characterization of a large variety of analytes. The use of this technique, however, presents great challenges in semiquantitative applications because of the complex phenomena occurring at the TLC surface. In our laboratory, we recently observed that the ion intensities of several alkali adduct ions were significantly different between the top and interior layer of the TLC plate. This indicates that the integrity of the TLC surface can have an important effect on the reproducibility of TLC- MALDI analyses. Graphical Abstract MALDI imaging reveals that surface integrity affects the detection of alkali adductions in TLC-MALD
Interferenza nella determinazione ABO su colonna in due casi di MEN da incompatibilità Rh
No full text- …
