403 research outputs found
Subjects with venous insufficiency have high risk of obstructive sleep apnea in relationship to fluid shift
No full textBackground: Obstructive sleep apnea (OSA) is highly prevalent in conditions characterized by fluid overload. Chronic venous insufficiency (CVI) is associated to fluid overload that might interfere with OSA occurrence and severity. Methods: A total of 180 patients with symptomatic CVI completed the Berlin questionnaire and were assessed for the presence of symptoms and signs of OSA and fluid shift. Results: According to the Berlin questionnaire, 59 patients (33%) had high risk of OSA. The predictors of having a positive Berlin questionnaire were male sex, body mass index and symptoms possibly related to fluid shift. Conclusions: Patients with CVI, a disease characterized by fluid overload, present high risk of OSA, which might be related to fluid shift
Ignacy Jan Paderewski - an apostle of polish independence, personification of uncommon intelligence, legendary piano virtuoso from Riond-Bosson
No full textIgnacy Jan Paderewski – „King of tones” and son of great Polish culture, was an excellent composer and pianist, who, as an interpreter of Chopin’s music and his own works, delivered speeches during numerous journeys before public performances at concerts, lobbying for Poland.
Out of the need of a patriotic heart, this esteemed diplomat and outstanding politician who demanded independence for Poland, actively supported various initiatives, including large sums for charity and public purposes. Despite the fame and honour of the whole world, he had a great dream to use the money he earned to buy a manor and agricultural property.
In this article, the author presents the Swiss headquarters in Riond-Bosson, which has become not only an important centre of a thriving Polish political emigration, but a forge of Polish living art and outstanding individuality.
In the suburbs of Morges, in the Paderewski villa, master classes for Polish pianists preparing to participate in the Chopin Competitions in Warsaw were held, as well as meetings during which Polish political and cultural history was shaped for a long time
Nils Bosson Sture. Historisk roman i tre samlingar.
No full text1. samling. Guldhalsbandet.Mode of access: Internet
Looking for chiral recognition in photoinduced bimolecular electron transfer using ultrafast spectroscopy
No full textdata published in:
Looking for chiral recognition in photoinduced bimolecular electron transfer using ultrafast spectroscopy, P. Verma, C. Nançoz, J. Bosson, G. M. Labrador, J. Lacour and E. Vauthey, Phys. Chem. Chem. Phys. 2023, 25, 11111-11120, https://doi.org/10.1039/D3CP00760
Allelic imbalance and somatic mutations in folate pathway genes in childhood acute lymphoblastic leukaemia
No full textABSTRACT Acute lymphoblastic leukaemia (ALL) is the commonest of the childhood cancers, but thankfully responds well to chemotherapeutic agents, with 80% of children achieving long-term survival. However, for the remaining 20% who relapse, outcome is bleak. Increasing knowledge and understanding of pharmacogenetics indicates that constitutive or acquired resistance to the drugs used in the treatment of cancer contribute to relapse. Methotrexate (MTX) is one of the most important drugs used in the treatment of ALL and the work presented here contributes to the body of knowledge relevant to the understanding of resistance to this drug. The aim of this research is to determine if changes in genes involved in folate metabolism contribute to methotrexate resistance and subsequent relapse of childhood ALL. The reduced folate carrier (RFC) is required to transport methotrexate into the cell where it competitively inhibits dihydrofolate reductase (DHFR) and other key enzymes of folate and 1-carbon metabolism. Following the design of primers and optimisation of PCR amplification, the entire coding regions of both RFC and DHFR were screened for novel SNPs or mutations using denaturing high performance liquid chromatography (DHPLC) on genomic DNA from 40 normal and 40 relapse childhood ALL patients. Ethical approval for use of the samples was granted under WCRLEC no347 and reference 2002/111 for normal and relapse samples respectively. The relapse group was made up of 29 males and 11 females with an average age of 6.59 years (range 0.8-14.1) and was made up of 5 T-cell; 30 B-cell; 4 mixed lineage; and 1 null classification. The screening method was shown to be sufficiently sensitive to detect single base changes. Several of the exons in both genes have a high G-C content and required destabilisation agents and/or use of a new DNA polymerase (OptimaseTM) to achieve sufficient PCR amplification. Dimethyl sulphoxide (DMSO) and betaine were shown to be effective agents which did not interfere with subsequent DHPLC. The results show that somatic mutations in the coding sequences of RFC and DHFR are rare in relapsed ALL and that while it is recognised as a mechanism of methotrexate resistance in vitro, it is unlikely to contribute to relapse in children with ALL. However, for one patient who suffered multiple relapses, a novel acquired mutation was identified in the 5'-UTR of the RFC-1 gene (C-37T). The significance of the C-37T mutation on RFC transcription requires further study, but it may decrease RFC mRNA quantity or stability and thus protein levels. DHPLC analysis also detected common SNPs. In terms of frequency, there were no significant differences between relapse and normal samples for the genotypes; RFC G80A (38.3% G/G; 49.4% G/A; 12.3% A/A in 81 normal samples; 31.8%; 56.8%; 11.4% respectively in 44 relapse samples; x2 p = 0.656); RFC C696T (97.8% C/C; 2.2% C/T; 0% T/T in 45 normal samples; 92.5%; 5%; 2.5% respectively in 40 relapse samples; x2 p = 0.458). However, there was a significant difference in the 5'-UTR -19 base pair deletion in the DHFR gene between the normal and relapse groups (0% WT/WT; 100% WT/-19 or -19/-19 in 45 normals; 26.7% and 73.3% respectively in 15 relapse samples; Fisher's exact probability p = 0.017) and needs to be confirmed in a larger cohort. Estimated copy number and the probability of loss of heterozygosity (LOH) were generated from the Affymetrix 50K SNP microarray for the RFC, DHFR and other genes involved in folate metabolism using gDNA from 73 presentation and 20 relapse childhood ALL cases. Mann-Whitney non-parametric comparison of the presentation and relapse data showed that for estimated copy number, statistically significant differences were seen for MTHFR (p = 0.0072), MS (0.0025), FPGS (0.00048), TS (0.00046), CBS (0.0002) and RFC (0.0001). When the data at the nearest SNP location to the gene was presented as a scatterplot, the difference in each case was due to a bimodal distribu..
Genome-wide identification of Ago2 binding sites from mouse embryonic stem cells with and without mature microRNAs
No full textMicroRNAs (miRNAs) are 19–22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer[superscript −/−] mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3′ untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.Leukemia & Lymphoma Society of AmericaUnited States. Public Health Service (Grant R01-GM34277)United States. Public Health Service (Grant R01-CA133404)National Cancer Institute (U.S.) (Grant P01-CA42063)National Cancer Institute (U.S.) Cancer Center Support (Grant P30-CA14051
Idun (Årg. 15, N:r 19)
No full textPultavamonumentet och dess skapare. Ernst Högman 293
Vår i museet. Heméra 294
Riksdagsöfversikt. I. 295
Jesu leende. Berättelse. Maria Durling 295
Provencalska vårdagar. II. Olof Bosson 298
En dröm. Helmy Rinders 299
Hjärtesorg. Snorre 299
Vårt legationshotell i Paris. Edvard Stjernström 300
Svensk läderplastik. 302
Litteratur. J. N-g. 303
Rösträttsprat från Göteborg. Dina 303
Ur dagskrönikan. 304
Ett konstnärligt hem på landet. E. H-n. 304
Teater och musik. 306
Köksalmanack. Redigerad af Fackskolan för huslig ekonomi i Upsala. 307
Läkareråd. 307
Frågor. 308
Svar. 308
Tidsfördrif. 309
Breflåda. 31
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