574 research outputs found
Hypergravity effects on proliferation and differentiation of C2C12 muscle-like cells
No full textThis study aimed at the investigation of muscle-like cell behaviour in conditions of altered gravity. C2C12 cells underwent stimulations by different hypergravity intensities (5, 10, 20 g) in the Large Diameter Centrifuge of the European Space Agency (ESA), and their features in terms of proliferation and differentiation were compared to control cultures carried out at normal earth gravity force. Proliferation was investigated determining the DNA content in the cultures. A positive correlation between DNA concentration (and therefore cell proliferation) and g values was found. Moreover, actin staining allowed for a qualitative study of the cytoskeleton rearrangement following hypergravity exposure. Differentiation was evaluated on confluent cultures treated with analogous protocol. Also in this case, hypergravity seems to positively affect the differentiation process of C2C12 cells and their fusion in myotubes. The evaluation of myosin expression by immunocytochemistry suggested an accelerated differentiation process following exposure of cells to different g values
Nanocomposite thin films based on polyethylene vinyl acetate and piezoelectric nanomaterials
No full text
The importance of adhesion control in crack-bridging measurements
No full textThe crack bridging ability (CBA) of two commercial coatings based on polyurethane resins was studied as specified in the European Standard EN test method. The test pieces of concrete blocks were used for the CBA evaluation at room temperature and the roughness index of the substrate was not measured. The results show that CBA resulted at lower values of displacement in the first coating than in the second one, the CBA values between 0.8 mm and 2.4 mm were observed. The results obtained at low temperature of 269 K show hat the mechanical response of the coating differs at ambient temperature as the low temperature causes the material to harden. The CBA values ranged between 0.42mm and 0.66 mm for the test performed at ambient temperature, and between 0.52 mm and 0.55 mm for the low temperature tests
Considerazioni e tecnica chirurgica a proposito del trattamento delle neoplasie palpebrali
No full textElectrochemotherapy: an effective local treatment of cutaneous and subcutaneous melanoma metastases
No full textTreatment of multiple cutaneous and subcutaneous melanoma metastases is still represents a therapeutic challenge for both dermatologists and oncologists. Electrochemotherapy (ECT) is a promising therapeutic procedure, owing to its ability to improve the penetration of cytotoxic drugs into cancer cells by application of current electric pulses. The aim of our study is to evaluate efficacy, tolerability and long-term efficacy of ECT in the treatment of advanced metastatic melanoma. Thirty patients affected by a total of 654 cutaneous and subcutaneous melanoma metastatic nodules were recruited. All patients were treated after they had undergone to a mild general anesthesia. Intravenous Bleomicina solution was administered 8 minutes before the application of electric pulses, generated by a Cliniporator (TM) (the device validated for ECT). The objective response rate of 100% (67.28% complete response and 32.72% partial response) was observed. A total of 214 metastatic lesions from 24 patients received a second ECT session, among them 141 showed a further complete response. Twenty-four months later, the local tumor control rate was 72%. The results of this study seem to demonstrate that ECT is an effective and valid therapeutic tool for the treatment of cutaneous metastases from melanoma. ECT can be considered a first-line palliative treatment since it is able to alleviate pain and reduce the tumor's spontaneous bleeding with a significant improve of patients' quality of life
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