421 research outputs found
Comparative analysis of beta-adrenergic receptor kinase and beta-arrestin mRNA expression in human cells.
No full textReceptor phosphorylation is a key step in the process of rapid desensitization. beta-Adrenergic receptor kinase is a specific receptor kinase that is known to phosphorylate and induce desensitization of several G-coupled synaptic receptors only when they are occupied by their agonists. We recently cloned human beta ARK cDNA and reported high levels of beta ARK expression in human peripheral blood leukocytes, also providing the first evidence for its possible functional role in these cells. Complete homologous receptor desensitization by beta ARK requires an additional cytosolic factor, called beta-arrestin. In the present study, we have cloned a 212 bp fragment of the human beta-arrestin cDNA to perform a comparative analysis of beta ARK and beta-arrestin mRNA expression in various human cell types. We found that also beta-arrestin mRNA is abundant in non-innervated tissues and cells. The fact that the entire machinery for G-coupled receptor desensitization is highly expressed in these cells further supports the idea that beta ARK may regulate nonsynaptic as well as synaptic receptors
Letter by Santilli et al regarding article, "Traditional risk factors are not major contributors to the variance in carotid intima-media thickness".
No full textRegulation of G protein-coupled receptor kinase subtypes in activated T lymphocytes. Selective increase of beta-adrenergic receptor kinase 1 and 2.
No full textMolecular cloning, functional expression and mRNA analysis of human beta-adrenergic receptor kinase 2.
No full textIn the present study the cDNA of human beta ARK2 was cloned using both PCR and cDNA library screening, subcloned into an expression vector and transiently expressed in COS7 cells. The expressed kinase activity was approximately 40% as efficient as human beta ARK1 in phosphorylating bovine rod outer segments in vitro. Northern blot analysis of human and bovine mRNA revealed a species-specific pattern of multiple hybridization bands, with two major transcripts in human rather than one in bovine. High levels of mRNA expression were found in peripheral blood leukocytes
La dimensione operativa psicologico-clinica nell’ambito dell’infettivologia ospedaliera.
No full textImpact of comorbidity on the risk and cost of hospitalization in HIV-infected patients: real-world data from Abruzzo Region [Corrigendum]
No full textCammarota S, Citarella A, Manzoli L, Flacco ME, Parruti G. Clinicoecon Outcomes Res. 2018;10:389–398. Page 393, Table 2, CCI scorec (vs 0), ≥2 row, the values for the Unadjusted IRR and Adjusted IRR columns are incorrect, the data “2.32 (1.79–3.01)” should read “2.87 (2.22–3.72)” and “2.04 (1.56–2.66)” should read “2.43 (1.86–3.17)”. Read the original article 
High expression of beta-adrenergic receptor kinase in human peripheral blood leukocytes. Isoproterenol and platelet activating factor can induce kinase translocation
No full textReceptor phosphorylation is a key step in the process of desensitization of the beta-adrenergic and other related receptors. A selective kinase (called beta-adrenergic receptor kinase, beta ARK) has been identified which phosphorylates the agonist-occupied form of the receptor. Recently the bovine beta ARK cDNA has been cloned and the highest levels of specific mRNA were found in highly innervated tissues. It was proposed that beta ARK may be primarily active on synaptic receptors. In the present study, the cDNA of human beta ARK was cloned and sequenced. The sequence was very similar to that of the bovine beta ARK (the overall amino acid homology was 98%). Very high levels of beta ARK mRNA and kinase activity were found in peripheral blood leukocytes and in several myeloid and lymphoid leukemia cell lines. Since agonist-induced beta ARK translocation is considered the first step involved in beta ARK-mediated homologous desensitization, we screened a number of G-protein-coupled receptor agonists for their ability to induce beta ARK translocation. In human mononuclear leukocytes, beta-AR agonist isoproterenol and platelet-activating factor were able to induce translocation of beta ARK from cytosol to membrane. After 20 min of exposure to isoproterenol (10 microM), the cytosolic beta ARK activity decreased to 61% of control, while membrane-associated beta ARK activity increased to 170%. 20-min exposure to platelet-activating factor (1 microM) reduced the cytosolic beta ARK activity to 42% of control with concomitant increase in membrane beta ARK activity to 214% of control. The high levels of beta ARK expression in human peripheral blood leukocytes together with the ability of isoproterenol and platelet-activating factor to induce beta ARK translocation, suggest a role for beta ARK in modulating some receptor-mediated immune functions
ALUI AND HAEIII RESTRICTION ENZYME BANDING-PATTERNS OF MACACA-FUSCATA AND CERCOPITHECUS-AETHIOPS-SABAEUS CHROMOSOMES
No full textAluI and HaeIII restriction endonuclease banding patterns were analyzed in Macaca fuscata and Cercopithecus aethiops sabaeus chromosomes. AluI produced C-negative bands in both species of monkeys, while HaeIII induced the appearance of C-negative bands on Macaca chromosomes and of simultaneous G + C bands on Cercopithecus metaphases
Functional capacity after traditional Chinese Medicine (Qi Gong) training in patients with chronic atrial fibrillation: a randomized pilot study.
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