47 research outputs found
A New In Vitro Model to Evaluate Anti-Adhesive Effect against Fungal Nail Infections
No full textNail fungal infection is often mistakenly considered a minor issue or a purely esthetic problem that is not worth solving. However, onychomycosis has been demonstrated to have a negative impact on a patient’s social life. Therefore, given the poor efficacy of various therapy types, there is strong interest in exploring new methods for evaluating antifungal treatments. As such, the
aim of this work was to develop a new protocol, using bovine hoof membranes as a model of the human nail to evaluate the capability of a product claiming to prevent fungal adhesion, which is the first step of the infection. In this work, two specific and representative fungal strains, Trichophyton rubrum and Candida albicans, were used. In order to evaluate the possible protective activity of a product against fungal contamination of the nail plate, it was first necessary to test the affinity of
the hoof membranes to be contaminated by the fungi. Then, a pharmaceutical product and a base coat were tested as a positive and negative control, respectively, by introducing the membranes (anti-fungal, basic or no treatment and single vs. multiple treatments) into the fungal suspensions for three different contact times (15 min, 5 h and 24 h). The results showed that the more significant antiadhesive effect (AE) was obtained against Trichophyton rubrum than against Candida albicans.
Furthermore, taking into account the results obtained at all testing times, 5 h appeared to be the best time for testing the antiadhesive activity. The results obtained after three treatments with drugs and on washed membranes, in comparison to one single application of antifungal product (AP), demonstrated clearly that the drug was able to penetrate deeper into the membranes to exert itself, even after washing and also after only 15 min of contact. Thus, hoof membrane has been shown to be a valuable in vitro model for this kind of product assessment
Enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine.
No full textThe performance of an enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine is described. Phosphate ions react with inosine in the presence of purine nucleoside phosphorylase to form hypoxanthine; this is oxidized by xanthine oxidase to uric acid with production of hydrogen peroxide. The latter is determined with the aid of the chromogen system peroxidase/4-aminophenazone/N-ethyl-N-(3-methylphenyl)-N'-acetyl- ethylenediamine, the coloured product being measured at 555 nm. This series of reactions is completed in 5 min at 37°C. The test is linear up to 240 mg/l. Analytical recovery in serum averaged 101.2 ± 1.2% and in urine 101.9 ± 3.2%. Within-run and between-run precision studies in serum and urine samples gave CVs ≤ 4.54% (at 22.0 mg/l). Results obtained by this method agree (r = ≥ 0.983) with the molybdate UV and molybdenum blue methods. Interference by endogenous substances, including organic phosphate, was negligible
Physical chemical characterization of binary systems of triacethyl gamma-cyclodextrins with tosylate salts of vancomycin and teicoplanin.
No full textCaratterizzazione ecologica ed ecofisiologica di Cistus salvifolius L. nelle Alpi Lombarde.
No full textHome-made Refurbishment of the Instrumentation and Control System of the TRIGA Reactor of the University of Pavia
No full textThe Instrumentation and Control (I&C) System of the TRIGA reactor of the University of Pavia was dated (see Fig.1) and, in order to grant a safe and continuous reactor operation for the future, it became necessary to substitute or to upgrade the system. Since the substitution of the I&C system with a new-made one was very difficult to be performed due to long authorization procedures, an home-made refurbishment was planned. Using commercial components of high quality, almost a complete substitution, channel-by-channel, of the I&C system was realized without changing the operating and safety logics. The refurbished I&C system shows a very good operational behavior and reliability and will assure a continuous operation of the reactor for the future
Ercolano e Pompei : raccolta generale de pitture, bronzi, mosaici, ec. : fin ora scoperti e riprodotti dietro le antichità di Ercolano, il Museo borbonico e le opere tutte pubblicate fin qui : accresciute da tavole inedite : con illustrazioni.
No full textIllustrated with etchings, both on single and folded leaves. Artists include G. Buttasson, B. Musitelli, L. Podestà, Gianantonio Zuliani, and Antonio Nardello.Preface of vol. 1 signed: L. Barré.Includes bibliographical references.v. 1. Decorazioni architettoniche -- v. 2. Composizione di molte figure, pt. 1 -- v. 3. Quadri, paesaggi -- v. 4. Figure isolate -- v. 5. Fregi, mosaici -- v. 6. Statue -- v. 7. [Other bronzework]Mode of access: Internet.Bound in old boards; gilt leather spines; sprinkled edges; bookplates of the VanderPoel Collection of Campanian Archaeology on front pastedowns
Il 'ritorno a Marx' nei “Quaderni del carcere” (1930)
No full textUno studio diacronico dei Quaderni del carcere e delle fonti utilizzate dal loro autore, mostra come in essi, in particolare nel corso del 1930, avvenga un vero e proprio "ritorno a Marx", che in precedenza era un autore da Gramsci non particolarmente frequentato, e non studiato e approfondito in dettaglio
Contact lenses as drug delivery systems of hemocomponents in the treatment of corneal lesions
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