165 research outputs found
Procedure di disinfezione in endoscopia digestiva: esperienze in un grande ospedale romano.
No full textThe Authors present the results of a survey on the disinfection procedures used in 13 endoscopy units of a large roman hospital. A questionnaire on the endoscopy centres characteristics and the methods of cleaning and disinfecting the equipment was adopted. Also microbiological samples on the instruments, disinfectants and rinsing water were carried out. Nine centres out of 13 presented one room and automatic washers were available only in two units.
Seven centres used a 0.4% glutaraldehyde with 1.41% phenol and 0.26% sodium phenate disinfectant (Sporicidin), three a high level disinfectant (2% glutaraldehyde) and three a peroxydic solution (Virkon). The disinfectant time of contact was <20’ only in eight cases. In 10 centres the biopsy forceps were processed as the endoscope. Only eight units were equipped with ventilation systems. In 12 endoscopes out of 31 P. aeruginosa or other bacteria were isolated. Furthermore in nine centres out of 13 high level disinfection was not achieved in at least one endoscope.
The results showed insufficient disinfection in several occasions because of a series of causes which are critically discussed
ERYTHROPOIETIN ALPHA VS BIOSIMILAR ERYTHROPOIETIN ALPHA PLUS LIPOFER AND B12 AND FOLATES IN PATIENTS WITH REFRACTORY ANEMIA. TWO CENTER PROSPECTIC STUDY
No full textORAL SEAWEED CALCIUM DERIVED FROM LITHOTHAMNION IS SAFE AND EFFECTIVE AND WELL TOLERATED AS ORAL LACTATE-GLUCONATE AND CARBONATE CALCIUM IN TUMOR LYSIS SYNDROME WITH RENAL FAILURE AND WATER OVERLOAD
No full textAbstracts of the 2012 International MASCC/ISOO (Multiple Association of Supportive Care in Cancer/International Society for Oral Oncology) Symposium. New York City, New York, USA. June 28-30, 2012
No full textDetermination of 15 organophosphorus pesticides residues in milk by dual column capillary gas chromatography
No full textA previous method was improved in order to determine 15 organophosphorus pesticides (OPPs) in raw milk by dual column capillary gas chromatography using Nitrogen-Phosphorus Detection (NPD).
With a single injection it was possible to measure the following OPPs: acephate, azinphos ethyl, chlorpyriphos ethyl, chlorpyriphos methyl, demeton-S-methyl sulfoxide, diazinon, disulfoton, methacrifos, methamidophos, methidation, parathion ethyl, phorate, pirimiphos methyl, pyrazophos, triazophos. All of these substances have a maximum residue levels (MRL) fixed by the European Commission.
The isolation was performed by liquid partition followed by a single cleanup with a C18 monofunctional solid phase cartridge
The GLC analysis was carried out with two capillary columns of different polarity, connected in parallel by a glass dual column adapter. The chromatographic temperature program was from 80°C to 100 °C at 2°C/min., followed by a second rate of 5°C/min. up to 300°C.
The quantification was obtained using parathion methyl as an internal standard.
The analyte identification was confirmed by comparing the retention times in the two columns.
The recovery and precision for each compound were determined using 18 aliquots of a blank milk. The aliquots were divided in 3 groups of six and fortified using 0.5, 1 and 1.5 times the permitted limit each, according to the European Commission Decision 2002/657/EC.
Although there was an increase in the number of analytes, the GLC conditions were further simplified without any changes in the analytic performances.
The method was developed in a UNI EN ISO 9001:2000 certified laboratory
Liposomial iron has an anti-inflammatory effect and is better than iron sulfate in correction of anemia of chronic inflammatory disease of young women
No full textHaematologica 2013, 98 (s1), pag 71
Prevenzione delle infezioni ospedaliere in una terapia intensiva.
No full textAim of the study was to evaluate the efficacy of the hospital infection preventive procedures adopted in the intensive care unit (ICU) of the "S. Camillo-Forlanini" hospital in Rome. First the following prevention protocols were analysed: invasive procedures (intubation, CVC and urinary catheter), surveillance cultures, infection management and antimicrobial prophylaxis. Comparison with international guidelines was carried out and protocols enforcement by the personnel was verified. Secondly a one year longitudinal surveillance study was performed in order to monitor the following site-specific infection rates: pneumonia (PNE), blood stream infections (BSI), urinary tract infections (UTI), surgical site infections (SSI). According to CDC definitions all patients developing infection 48 hours or more after ward admission were included. Furthermore risk factors (i.e. age, sex, SAPS II), invasive procedures (i.e. endotracheal intubation, vascular and urinary catheterisation), microbiological isolates and their antibiotic susceptibility were screened. Overall 302 patients (191 men and 111 women) were admitted; age 55.1 +/- 20.7 years (mean), SAPS II 42.4 +/- 16.2 (mean) and average ward stay 12.5 +/- 21.7 days. Crude mortality was 15.9%. Results showed a total of 37 infection episodes (20 PNE , 7 BSI, 8 UTI and 2 SSI) in 33 patients (10.9%). Infection and mortality rates were among the lowest registered in other italian ICU's. Standardized infection rates associated to invasive procedures were: Ventilator-associated PNE rate (7.8/1000), central venous catheter-associated BSI rate (2.2/1000), urinary catheter-associated UTI rate (2.1/1000). The first (PNE) was higher than the NNIS mean rate, whwreas BSI and urinary catheter associated rates were minor than the mean rates reported by NNIS. Gram-negatives were 61.7%, gram-positives 27.6% and Candida spp. 10.6%. The results confirm the ICU successful preventive strategy
Erythropoietin alpha vs biosimilar erythropoietin alpha plus lipofer, B12 and folates in patients with refractory anemia: two center prospective study
No full textHaematologica 2012, 97 (s1), pag 14
Residue analysis of organophosphorus pesticides in animal matrices by dual column capillary gas chromatography with nitrogen-phosphorus detection
No full textOrganophosphorus pesticides (OPPs) were determined in matrices of animal origin by dual column capillary gas chromatography using Nitrogen-Phosphorus Detection (NPD).
This method was tested on cow milk and on liver and muscle of wild boar.
The isolation of these pesticides was performed by liquid partition followed by cleanup with solid phase cartridge (SPE C18), after extraction from the matrix.
The analytes identification was obtained by comparing the retention times in two columns with different polarity.
The quantification of each OPP was obtained using parathion-ethyl as internal standard.
The method was developed in a UNI EN ISO 9001:2000 certified laboratory.
The recovery, investigated by analyzing samples spiked at 5, 10 and 50 ppb, ranged from 59% to 117% in milk, from 60% to 81% in liver and from 68% to 76% in muscle.
The limit of quantification (LOQ) and limit of detection (LOD) were respectively 5 ppb and 1 ppb for each compound and allowed quantifying the residues below the legal limits
Hollow-Core-Fiber Placement in Latency-Constrained Metro Networks with edgeDCs
No full textWe investigate the optimal placement of Hollow-Core Fibers (HCF) in latency-constrained metro networks with edgeDCs, performing physical-layer validation. Upgrading 24% of links to HCF reduces edgeDCs number by 29% compared to a network without HCFs
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