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    FIGURE 6 in A new Dondice Marcus Er. 1958 (Gastropoda: Nudibranchia) from the Mediterranean Sea reveals interesting insights into the phylogenetic history of a group of Facelinidae taxa

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    FIGURE 6 Geographical distribution of the Mediterranean D. trainitoi sp. nov. with the red stars indicating the collection localities. 1= Punta del Faro, Portofino promontory, Liguria (44°17'53''N, 9°13'47''E), 2= 'Asia' wreck, Civitavecchia, Latium (42°03'15''N, 11°47'45''E), 3= Procida Island, Naples (40°45'02.6"N, 14°01'31.6"E).Published as part of <i>Furfaro, Giulia & Mariottini, Paolo, 2020, A new Dondice Marcus Er. 1958 (Gastropoda: Nudibranchia) from the Mediterranean Sea reveals interesting insights into the phylogenetic history of a group of Facelinidae taxa, pp. 1-22 in Zootaxa 4731 (1)</i> on page 14, DOI: 10.11646/zootaxa.4731.1.1, <a href="http://zenodo.org/record/3635551">http://zenodo.org/record/3635551</a&gt

    Figure 5 in Ecological notes of the alien species Godiva quadricolor (Gastropoda: Nudibranchia) occurring in Faro Lake (Italy)

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    Figure 5. (a) Cytochrome oxidase subunit I (COI) haplotype TCS network showing genetic mutations occurring within Godiva quadricolor specimens; (b) Bayesian topology based on the COI data set (TrN +G model of evolution). Numbers at nodes indicate the support by Bayesian Inference (5 × 106 generations and 25% burn-in, left half) and Maximum Likelihood (1000 bootstrap replicates, right half).Published as part of Furfaro, Giulia, De Matteo, Sergio, Mariottini, Paolo & Giacobbe, Salvatore, 2018, Ecological notes of the alien species Godiva quadricolor (Gastropoda: Nudibranchia) occurring in Faro Lake (Italy), pp. 645-657 in Journal of Natural History 52 (11-12) on page 652, DOI: 10.1080/00222933.2018.1445788, http://zenodo.org/record/517429

    The southernmost record of Felimida elegantula (Philippi, 1844) (Gastropoda: Nudibranchia)

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    ABSTRACT: The southernmost record of the Mediterranean nudibranch Felimida elegantula (Philippi, 1844) is reported here. A single specimen, with a colour pattern that hampered an easy morphological identification, was collected at the Kerkennah Islands (Tunisia). DNA-barcoding analysis (with the cytochrome c oxidase subunit I genetic marker) unambiguously confirmed its identification, and radular and anatomical features conformed with those previously described. With this record, the chromatic polymorphism of F. elegantula is demonstrated to be extremely large and the known range of the species is extended to the African coast of Tunisia. © 2016, Senckenberg Gesellschaft für Naturforschung and Springer-Verlag Berlin Heidelberg

    Figure 1 in Ecological notes of the alien species Godiva quadricolor (Gastropoda: Nudibranchia) occurring in Faro Lake (Italy)

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    Figure 1. Italian distribution of Polycera hedgpethi (letters, square) and Godiva quadricolor (numbers, circles). 1: north Adriatic lagoon, Zenetos et al. (2016); 2: Noli, Ligurian Sea, Betti et al. (2015); 3: Sabaudia Lake, Macali et al. (2013); 4: Fusaro Lake, Gulf of Naples, Cervera et al. (2010); 5: Faro Lake, Sicily, present study. A: Sacca Sessola Island, Venice Lagoon, Italy (Keppel et al. 2012); B: Marina di Ravenna docks, Italy (Rudman 2005; Trainito 2005); C: Rimini coast, Italy (Ioni, 2011); D: Fusaro Lake, Gulf of Naples, Cervera et al. 1991 (1988)); E: Faro Lake, Sicily, Giacobbe and De Matteo (2013).Published as part of Furfaro, Giulia, De Matteo, Sergio, Mariottini, Paolo & Giacobbe, Salvatore, 2018, Ecological notes of the alien species Godiva quadricolor (Gastropoda: Nudibranchia) occurring in Faro Lake (Italy), pp. 645-657 in Journal of Natural History 52 (11-12) on page 646, DOI: 10.1080/00222933.2018.1445788, http://zenodo.org/record/517429

    Traveling around the Mediterranean Sea: the bryozoan Amathia verticillata (Delle Chiaje, 1822) together with non-indigenous and endemic overlooked nudibranchs (Mollusca, Gastropoda)

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    The Mediterranean Sea is recognized as a hot spot of biodiversity, but the ever-increasing commercial and recreational marine traffic and global warming pose significant threats to its fauna by enabling and facilitating, respectively, the introduction of non-indigenous species (NIS). To mitigate negative effects in the native communities, greater efforts are being made to generate updated lists of NIS and to monitor their spread over space and time. In this regard, Amathia verticillata (delle Chiaje, 1822), a worldwide distributed arborescent bryozoan, can play an important role as a vector of NIS due to its ability to be a species ‘carrier of other species’. Despite its importance, a detailed study on the highly specialized Nudibranchia molluscs associated with A. verticillata is still lacking. Given that some nudibranchs serve as powerful bioindicators of changes in the Mediterranean Sea, and considering that A. verticillata is an invasive species capable to facilitating the establishment and spread of NIS, a broad bibliographic study coupled with field investigations in different anthropized areas of the Central Mediterranean Sea were conducted. The nudibranch fauna associated with A. verticillata in the Mediterranean Sea was assessed for the first time. Nine species were found associated with this bryozoan, five of which were directly observed and analysed using morphological and molecular techniques. A case of cryptic diversity was revealed, with Polycerella recondita Schmekel, 1965 resurrected as a valid species, and the neglected Tenellia granosa (Schmekel, 1966) reported for the fourth time in the Mediterranean Sea. Notably, only three out of the nine species are Indo-Pacific, while the remaining six taxa have an Atlantic/Mediterranean distribution. This finding prompted further phylogenetic and haplotype network analyses on the two nudibranch species directly associated with A. verticillata: Bermudella polycerelloides and P. recondita. These analyses revealed different and previously unknown ecological traits of the spread of these two species

    Ecology and distribution of the Mediterranean Dondice trainitoi Furfaro and Mariottini, 2020 (Mollusca: Nudibranchia)

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    During scientific technical dives in a marine animal forest (MAF) environment, the nudibranch Dondice trainitoi Furfaro & Mariottini 2020 was recorded on a rocky bottom at 50 m depth in the Salento Peninsula, Southern Italy, Eastern Mediterranean Sea. Further in situ explorations and broad bibliographic research within both the scientific and grey literature revealed that D. trainitoi is widely distributed across the Mediterranean Sea, despite what was previously known. Herein are the first records for the Adriatic Sea and the Apulia region of Italy, together with the deepest record of this species. An analysis of collected samples and both in situ and laboratory photographs of the hydrozoan colonies associated with D. trainitoi revealed its ecology and trophic behaviour; we conclude that the hydrozoan Obelia bidentata Clark, 1875 is its preferred prey, but that it also feeds on the athecate hydroid Eudendrium glomeratum Picard, 1952. These findings shed some light on the little-known ecology and distribution, on both a geographical and a bathymetric scale, of this species, which are fundamental to increasing knowledge on Mediterranean biodiversity and its ecosystem functions

    On the occurrence of Porpita porpita in the Tyrrhenian Sea: COI and ITS2 DNA barcoding identification

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    The record of the Blue Button Porpita porpita (Linnaeus, 1758) (Cnidaria, Hydrozoa) in the Tyrrhenian Italian Sea (41°16'06.72" N, 13°01'34.13" E), is reported here for the first time. A single specimen was collected in the Latium (Central Italy) coast, and firstly identified on the base of its morphology (Fig.1). DNA barcoding analysis, with the use of the COI mitochondrial marker, unambiguously confirmed its identification (100 % homologous to the GenBank sequence with accession number GQ 120060). Additionally, the nuclear ITS2 genetic marker (Oliverio & Mariottini 2001) was used as a further barcoding sequence that revealed a high homology (ranging from 99 to 100 %) with sequences already present in GenBank but which were, surprisingly, never assigned until now (accession numbers: GU941198, GU941346, GU942419, KJ182875, KX113716, KX113680, KX115166, KX115168 and KX115212). The P. porpita COI and ITS2 nucleotide sequences were deposited at the EMBL/ GenBank with the accession number LT795124 and LT795123, respectively (voucher RM3_747). With this record the range of P. porpita within the Mediterranean basin is extended and furthermore, the use of the ITS2 as a valid DNA barcode marker for this group was here confirmed. This study represents a good example of an inverse molecular route for the identification of unassigned environmental samples (Lee et al. 2010)

    A new perspective on the distribution of Camachoaglaja africana (Pruvot-Fol, 1953) and Biuve fulvipunctata (Baba, 1938) (Gastropoda, Cephalaspidea) in the Mediterranean and the NE Atlantic

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    During underwater monitoring, one specimen of Camachoaglaja africana and one of Biuve fulvipunctata were found at the ‘Tavolara Punta Coda Cavallo’ Marine Protected Area, allowing to expand the known Mediterranean range of distribution of these two species. The finding of both species constitutes the first report for NE Sardinia and the whole Tyrrhenian Sea. By coupling data obtained from the analysis of social media and those present in the bibliography it was also possible to speculate on alternative pattern of distribution of these two species at a global scale. Finally, it is also confirmed the importance of repeated monitoring activities over time on the mooring buoys which act as steppingstone habitats for epifauna or for larval settlement and that are capable to provide important additional information on local biodiversity.&nbsp

    Integrative Taxonomy of the Bubble Snails (Cephalaspidea, Heterobranchia) Inhabiting a Promising Study Area: The Coastal Sicilian Faro Lake (Southern Italy)

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    The worldwide diffused bubble snails, Haminoeidae, although characterized by an extreme morphological homogeneity, display the most diverse radiation inside the order Cephalaspidea. This hidden diversity within the family Haminoeidae was recently unraveled by molecular studies, which helped to understand the evolutionary history of this group by clarifying some aspects of its systematics. In fact, the type genus Haminoea W. Turton and Kingston (1830) was proved to be polyphyletic and, consequently, the genus Haminoea sensu stricto was restricted to the Mediterranean, Atlantic and East Pacific species, with the Mediterranean Haminoea hydatis Linnaeus (1758) as the type taxon. However, at the specie rank, many aspects need to be clarified, especially concerning the Mediterranean fauna. Due to low reliability of macro-morphological characters, the minimal quantity of molecular data currently available on Mediterranean specimen adds to the lack of molecular comparison in most reports. Based on such considerations, Haminoea species from an interesting Mediterranean study area, Faro Lake, a Sicilian coastal lake that is considered a hot spot for both alien and endemic marine Heterobranchia, have been studied using an integrative taxonomic approach. Eleven Mediterranean specimens belonging to four Haminoea bubble snails have been collected, identified and compared with samples from other localities, integrating ecological, morphological, anatomical (reproductive apparatus) and molecular data. Based on molecular investigations carried out on three different molecular markers (H3, 16S and COI), the morphological identifications of the species collected in the Faro Lake have been confirmed, and 37 new sequences are provided for future comparisons. Furthermore, results from this integrative systematic study shed light on the phylogenetic relationships occurring in this group of bubble snails that could be useful in identifying valid diagnostic morphological characters. Haminoea hydatis and H. navicula were confirmed to be close to each other, with H. orteai as sister to them and with H. orbignyana as the basal taxon. Given external morphological features are unreliable with species identification in Haminoea genus open questions on the geographical distribution of the species and on their ranges of intraspecific variability have yet to be addressed and further in-depth studies are needed. Finally, the presence of three sympatric Haminoea species, two of which are considered native or long-time naturalized, along with other occasional congeneric species, and the absence of the introduced invasive Haloa japonica, reflects both the resilience and stochastic space-temporal dynamics of Faro Lake. This confirms it as an inexhaustible source of case-studies
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