1,050 research outputs found
Professionalisation for New Zealand Career Practice: Lessons to Learn, Challenges to Meet.
The New Zealand Association of Counsellors has been engaged in a professionalisation process for counselling in New Zealand since the mid-1980s. The issues surrounding professionalisation have been well documented in the New Zealand Journal of Counselling. The Career Practitioners Association of New Zealand is now attempting to establish a professional environment for career practice in New Zealand. In this article, the author suggests that professionalisation for career practice involves many of the same issues that were salient for counselling, and that professionalisation of career practice would be facilitated by referring to counselling's experience. Critical issues and needed steps for professionalisation of career practice are further identified in the article. [ABSTRACT FROM AUTHOR] Copyright of New Zealand Journal of Counselling is the property of New Zealand Association of Counsellors and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.
Profiling of Soluble Neutral Oligosaccharides from Treated Biomass using Solid Phase Extraction and Liquid Chromatography-Multiplexed Collision Induced Dissociation-Mass Spectrometry
Thermochemical pretreatment of cellulosic biomass improves cell wall enzymatic digestibility, while simultaneously releasing substantial amounts of soluble oligosaccharides. Profiling of oligosaccharides released during pretreatment yield information essential for choosing glycosyl hydrolases necessary for cost-effective conversion of cellulosic biomass to desired biofuel/biochemical end-products. In this report we present a methodology for profiling of soluble neutral oligosaccharides released from ammonia fiber expansion (AFEXTM)-pretreated corn stover. Our methodology employs solid phase extraction (SPE) enrichment of oligosaccharides based on porous graphitized carbon (PGC), followed by high performance liquid chromatography (HPLC) separation using a polymeric amine based column (Prevail Carbohydrate ES) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF-MS) in both positive and negative modes. For structural elucidation on the chromatographic time scale, nonselective multiplexed collision-induced dissociation was performed for quasi-simultaneous acquisition of accurate molecular and fragment masses of neutral oligosaccharids in a single analysis. These analyses directly revealed presence of glucans up to degree of polymerization (DP) 22 without side-chain modifications. Additionally, arabinoxylans with DP up to 6 were detected in the pretreated biomass samples (post-enzymatic digestion). All linkages between sugar units in glucans and arabinoxylans were identified to be p-1-4 linkages based on cross-ring fragment masses. Comprehensive profiling of soluble oligosaccharides also demonstrated that arabinoxylan acetylation was reduced by greater than 85% post-AFEXTM treatment.Published version: Vismeh, Ramin, Humpula, James F., Chundawat, Shishir P. S., Balan, Venkatesh, Dale, Bruce E. & Jones, A. Daniel. (2013). Profiling of Soluble Neutral Oligosaccharides from Treated Biomass using Solid Phase Extraction and LC-TOF MS. Carbohydrate Polymers 94(2), 791-799. http://dx.doi.org/10.1016/j.carbpol.2013.02.00
Probing the nature of AFEX-pretreated corn stover derived decomposition products that inhibit cellulase activity
Sequential fractionation of AFEX-pretreated corn stover extracts was carried out using ultra-centrifugation, ultra-filtration, and solid phase extraction to isolate various classes of pretreatment products to evaluate their inhibitory effect on cellulases. Ultra-centrifugation removed dark brown precipitates that caused no appreciable enzyme inhibition. Ultra-filtration of ultra-centrifuged AFEX-pretreated corn stover extractives using a 10 kDa molecular weight cutoff (MWCO) membrane removed additional high molecular weight components that accounted for 24–28% of the total observed enzyme inhibition while a 3 kDa MWCO membrane removed 60–65%, suggesting significant inhibition is caused by oligomeric materials. Solid phase extraction (SPE) of AFEX-pretreated corn stover extractives after ultra-centrifugation removed 34–43% of the inhibition; ultra-filtration with a 5 kDa membrane removed 44–56% of the inhibition and when this ultra-filtrate was subjected to SPE a total of 69–70% of the inhibition were removed. Mass spectrometry found several phenolic compounds among the hydrophobic inhibition removed by SPE adsorption.Published version: Humpula, James F., Uppugundla, Nirmal, Vismeh, Ramin, Sousa, Leonardo, Chundawat, Shishir P. S., Jones, A. Daniel, Balan, Venkatesh, Dale, Bruce E. & Cheh, Albert M. (2014). Probing the nature of AFEX-retreated corn stover derived decomposition products that inhibit cellulase activity. Bioresource Technology 152, 38-45. http://dx.doi.org/10.1016/j.biortech.2013.10.08
Towards a Framework for Developing Mobile Agents for Managing Distributed Information Resources
Distributed information management tools allow users to author, disseminate, discover and manage information within large-scale networked environments, such as the Internet. Agent technology provides the flexibility and scalability necessary to develop such distributed information management applications. We present a layered organisation that is shared by the specific applications that we build. Within this organisation we describe an architecture where mobile agents can move across distributed environments, integrate with local resources and other mobile agents, and communicate their results back to the user
Nutrient management guidelines for Russet Burbank potatoes
Bulletin no. 840 Moscow, Idaho :University of Idaho, College of Agriculture, Cooperative Extension System, 2004-10-01. Author(s): Stark, Jeff; Westermann, Dale; Hopkins, Bryan
The Pause that Refreshes: A Study of Self-Funded Leave
SelfÂfunded leave programmes provide a time resource to employees so that they can pursue interests outside their normal employment. This article reports the results of qualitative research conducted on 16 workers who completed a selfÂfunded leave programme. SemiÂstructured interviews were used to explore participants’ motivations to enrol in the selfÂfunded leave programme, how the leave contributed to workÂlife balance through development of nonÂwork life roles, and difficulties that arose during participation in the selfÂfunded leave programme. Results reveal that participants did use self funded leave to improve their workÂlife balance and augment other lifeÂroles. However, unpredicted events influenced how the leave time was actually used, which was often different from original plans and goals. Adult transition theory is used to conceptualize the process of participating in selfÂfunded leave and to suggest how employees who undertake selfÂfunded leave can be best supported by employers
Unifying Distributed Processing and Open Hypertext through a Heterogeneous Communication Model
A successful distributed open hypermedia system can be characterised by a scaleable architecture which is inherently distributed. While the architects of distributed hypermedia systems have addressed the issues of providing and retrieving distributed resources, they have often neglected to design systems with the inherent capability to exploit the distributed processing of this information. The research presented in this paper describes the construction and use of an open hypermedia system concerned equally with both of these facets
- …
