4,326 research outputs found
Effect of continuous exposure to 5-FU, 4-HC and 5-FU+4-HC on viability and invasiveness of 231/LM2-4 cells.
No full text(A) Effect of prolonged continuous exposures (six days) to different concentrations of 5-FU (0.1, 0.5, 1 and 10μM), 4-HC (0.01, 0.05, 0.1 and 1μM), and to the combination 1μM 5-FU + 0.01μM 4-HC, on in vitro viability of 231/LM2-4 cells. The relative absorbance (A490nm-A650nm) to control is represented; A490nm is directly proportional to the number of living cells and A650nm eliminates any kind of background. (B) Assessment of the invasive capacity of 231/LM2-4 cells, under untreated and treated conditions, in a transwell chemoinvasion assay using collagen type IV as coating. The protracted treatments with low dose and non-toxic doses of 1μM 5-FU, 0.01μM 4-HC and the 1μM 5-FU + 0.01μM 4-HC combination are detailed in Materials and methods. Cells that invaded and passed through membrane pores were stained with 0.1% crystal violet and counted. The numbers are the mean ± standard error of the mean (SEM), of the cell counts of eight random fields (x20 magnification) per sample of a representative experiment. The experiment was repeated three times. A significant reduction in invasive capacity of the cells was observed in all treated groups (***pvs. control). Significance was analyzed by one-way ANOVA with Dunnett’s post-test.</p
Inhibition of Hypoxia-Induced Increase of Blood-Brain Barrier Permeability by YC-1 through the Antagonism of HIF-1α Accumulation and VEGF Expression.
No full textObservation of hc radiative decay hc › ??' and evidence for hc › ??
No full textKolcu, Onur Buğra (Arel Author) --- Makale 69 yazarlıdır.A search for radiative decays of the P-wave spin singlet charmonium resonance hc is performed based on 4.48 × 108 ? events collected with the BESIII detector operating at the BEPCII storage ring. Events of the reaction channels hc › ??' and ?? are observed with a statistical significance of 8.4? and 4.0?, respectively, for the first time. The branching fractions of hc › ??' and hc › ?? are measured to be B(hc › ??' ) = (1.52±0.27±0.29)×10-3 and B(hc › ??) = (4.7±1.5±1.4)×10-4 , respectively, where the first errors are statistical and the second are systematic uncertainties
YC-1 attenuates LPS-induced proinflammatory responses and activation of nuclear factor-kappaB in microglia.
No full textFinancial analysis and evaluation of company HC Betons Ltd
No full textDiplomdarba nosaukums: Uzņēmuma SIA HC Betons finanšu analīze un novērtējums
Diplomdarba mērķis ir uz finanšu analīzes pamata izvērtēt uzņēmuma SIA HC Betons esošo finansiālo situāciju un izstrādāt priekšlikumus finansiālā stāvokļa uzlabošanai.
Teorētiskajā daļā apskatīti finanšu analīzes teorētiskie aspekti. Praktiskajā daļā autore veica uzņēmuma SIA HC Betons finanšu analīzi par 2009., 2010., un 2011.gadu. Analītiskajā daļā autore salīdzina rādītājus ar konkurējošo uzņēmumu SIA Cemex.
Autore secinājusi, ka uzņēmumam ir finansiālas grūtības, bet pēc autores domām, uzņēmums tuvāko gadu laikā nebankrotēs.
Diplomdarba apjoms ir 78 lapaspuses, tas sastāv no 3 daļām, 12 tabulām, 5 attēliem un 12 pielikumiem.
Atslēgvārdi: analīze, likviditāte, bankrots, koeficienti.Diploma thesis: Financial Analysis and Evaluation of Company HC Betons Ltd.
The aim of the diploma paper is to evaluate company’s HC Betons Ltd current financial situation based on financial analysis and make suggestions for improvement of the financial position.
Theoretical aspects of financial analysis are discussed in the theoretical part of the paper. In the practical part author has performed company’s HC Betons Ltd financial analysis of the years 2009, 2010 and 2011. In the analytical part author compares ratios of a competing company Cemex Ltd.
The author concludes that the company is in financial difficulties, but according to the author’s thoughts, the company will not bankrupt in the coming years.
The volume of the diploma paper is 78 pages; it consists of 3 parts, 12 tables, 5 images and 12 appendices.
Keywords: analysis, liquidity, bankruptcy, ratios
Metabolic profiling and population screening of analgesic usage in nuclear magnetic resonance spectroscopy-based large-scale epidemiologic studies
No full textThe application of a 1H nuclear magnetic resonance (NMR) spectroscopy-based screening method for determining the use of two widely available analgesics (acetaminophen and ibuprofen) in epidemiologic studies has been investigated. We used samples and data from the cross-sectional INTERMAP Study involving participants from Japan (n = 1145), China (n = 839), U.K. (n = 501), and the U.S. (n = 2195). An orthogonal projection to latent structures discriminant analysis (OPLS-DA) algorithm with an incorporated Monte Carlo resampling function was applied to the NMR data set to determine which spectra contained analgesic metabolites. OPLS-DA preprocessing parameters (normalization, bin width, scaling, and input parameters) were assessed systematically to identify an optimal acetaminophen prediction model. Subsets of INTERMAP spectra were examined to verify and validate the presence/absence of acetaminophen/ibuprofen based on known chemical shift and coupling patterns. The optimized and validated acetaminophen model correctly predicted 98.2%, and the ibuprofen model correctly predicted 99.0% of the urine specimens containing these drug metabolites. The acetaminophen and ibuprofen models were subsequently used to predict the presence/absence of these drug metabolites for the remaining INTERMAP specimens. The acetaminophen model identified 415 out of 8436 spectra as containing acetaminophen metabolite signals while the ibuprofen model identified 245 out of 8604 spectra as containing ibuprofen metabolite signals from the global data set after excluding samples used to construct the prediction models. The NMR-based metabolic screening strategy provides a new objective approach for evaluation of self-reported medication data and is extendable to other aspects of population xenometabolome profiling
Synergistic anti-tumor activity through combinational intratumoral injection of an in-situ injectable drug depot
No full textHere, we describe combinational chemotherapy via intratumoral injection of doxorubicin (Dox) and 5-fluorouracil (Fu) to enhance the efficacy and reduce the toxicity of systemically administered Fu and Dox in cancer patients. As the key concept in this work, mixture formulations of Dox-loaded microcapsules (Dox-M) and Fu-loaded Pluronic((R)) hydrogels (Fu-HP) or Fu-loaded diblock copolymer hydrogels (Fu-HC) have been employed as drug depots. The in vitro and in vivo drug depot was designed as a formulation of Dox-M dispersed inside an outer shell of Fu-HP or Fu-HC after injection. The Dox-M/Fu-HP and Dox-M/Fu-HC formulations are free flowing at room temperature, indicating injectability, and formed a structural gelatinous depot in vitro and in vivo at body temperature. The Fu-HP, Fu-HC, Dox-M/Fu-HP, Dox-M/Fu-HC, and Dox-M formulations were easily injected into tumor centers in mice using a needle. Dox-M/Fu-HC produced more significant inhibitory effects against tumor growth than that by Dox-M/Fu-HP, while Fu-HP, Fu-HC and Dox-M had the weakest inhibitory effects of the tested treatments. The in vivo study of Dox and Fu biodistribution showed that high Dox and Fu concentrations were maintained in the target tumor only, while distribution to normal tissues was not observed, indicating that Dox and Fu concentrations below their toxic plasma concentrations should not cause significant systemic toxicity. The Dox-M/Fu-HP and Dox-M/Fu-HC drug depots described in this work showed excellent performance as chemotherapeutic delivery systems. The results reported here indicate that intratumoral injection using combination chemotherapy with Dox-M/Fu-HP or Dox-M/Fu-HC could be of translational research by enhancing the synergistic inhibitory effects of Dox and Fu on tumor growth, while reducing their systemic toxicity in cancer patients
Specific and fitness testing of goalies HC Motor Czech Budejovice\\
No full textThe aim of this bachelor thesis is to test specific and fitness abilities of goalkeepers of HC Motor Czech Budejovice. In the theoretical part of this thesis, based on content analysis of literature research, the author presents motoric abilities, development patterns and overview of most frequently used tests. In the practical part of thesis, the author deals with creating and verifying tested battery. It was designed using the knowledge gained from books reference and self- experience of a player and a coach as well. To obtain the results of performance of particular motoric abilities the author used testing method. The outcomes were subsequently compared with the evaluation of given motoric abilities on ice. The author acquired the evaluation from goalkeeper's coaches of HC Motor Czech Budejovice. Final results are described and thereafter tabularly presented
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