95,849 research outputs found
Make fu yin chuan.
Special collection from London Missionary Society.; On double leaves, oriental style, in case.; Text in Guangzhou dialect.; Also available in an electronic version via the Internet at http://nla.gov.au/nla.gen-vn1809106.Bible. N.T.Mark's Gospel880-02 Xin yüe quan shu, Make chuan fu yin sh
Yuehan chuan fu yin shu.
Special collection from London Missionary Society.; On double leaves, oriental style, in case.; Texts in Guangzhou dialect.; Also available in an electronic version via the Internet at http://nla.gov.au/nla.gen-vn1803766.Bible. N.T.880-02 Xin yue quan shu, Yuehan chuan fu yin sh
Matai chuan fu yin shu zhu shi /
Special collection from London Missionary Society.; On double leaves, oriental style, in case.; Also available in an electronic version via the Internet at http://nla.gov.au/nla.gen-vn493353.880-02 Matai chuan fu yin zhu shi.Gospel of Matthew, in chinese.Bible. N.T. Matthew. Chinese. 1848
Ming Qing Hua yan chuan cheng shi liao liang zhong : "Xian shou zong cheng" yu "Xian shou chuan deng lu" /
Zong pai wen ti shi zhong guo fo jiao fa zhan li shi guo cheng dang zhong yi ge nan jie de xian xiang, zong guan zhong guo fo jiao, jing tu yi zong neng fou cheng li zhi jin reng ran ju song fen yun, tian tai yu chan zong you yu you wan zheng de wen xian ji lu, yuan liu chuan cheng xiang dui qing xi. Zhi yu xian shou zong de li shi fa zhan, duo shu fang fu meng long zhi zi, ran er suo xing jin nian xu duo chong yao shi liao xiang ji wen shi, ming qing yi lai xian shou zong fa xi chuan cheng yuan liu ji hu ke wei yun wu kuo qing, zai ci ji chu zhi shang, xue jie dui yu zhong guo fo jiao zong pai fa zhan li shi jin cheng de ren shi yi jing da fu gai xie. "Xian shou zong cheng" yu "xian shou chuan deng lu" shi ming qing yi lai liang bu zhong yao de hua yan chuan cheng shi liao, dui ren shi xian shou zong li shi fa zhan guo cheng ti gong yi ge zhong yao xian suo.宗派問題是中國佛教發展歷史過程當中一個難解的現象,綜觀中國佛教,淨土一宗能否成立至今仍然聚訟紛紜,天台與禪宗由於有完整的文獻記錄,源流傳承相對清晰.至於賢首宗的歷史發展,多屬彷彿朦朧之姿,然而所幸近年許多重要史料相繼問世,明清以來賢首宗法系傳承源流幾乎可謂雲霧廓清,在此基礎之上,學界對於中國佛教宗派發展歷史進程的認識已經大幅改寫."賢首宗乘"與"賢首傳燈錄"是明清以來兩部重要的華嚴傳承史料,對認識賢首宗歷史發展過程提供一個重要線索.Fu lu: fo xin ci ji miao bian da shi bie feng tong gong ta ming deng san zhong.Includes index.附錄: 佛心慈濟妙辨大師別峰同公塔銘等三種Zong pai wen ti shi zhong guo fo jiao fa zhan li shi guo cheng dang zhong yi ge nan jie de xian xiang, zong guan zhong guo fo jiao, jing tu yi zong neng fou cheng li zhi jin reng ran ju song fen yun, tian tai yu chan zong you yu you wan zheng de wen xian ji lu, yuan liu chuan cheng xiang dui qing xi. Zhi yu xian shou zong de li shi fa zhan, duo shu fang fu meng long zhi zi, ran er suo xing jin nian xu duo chong yao shi liao xiang ji wen shi, ming qing yi lai xian shou zong fa xi chuan cheng yuan liu ji hu ke wei yun wu kuo qing, zai ci ji chu zhi shang, xue jie dui yu zhong guo fo jiao zong pai fa zhan li shi jin cheng de ren shi yi jing da fu gai xie. "Xian shou zong cheng" yu "xian shou chuan deng lu" shi ming qing yi lai liang bu zhong yao de hua yan chuan cheng shi liao, dui ren shi xian shou zong li shi fa zhan guo cheng ti gong yi ge zhong yao xian suo.宗派問題是中國佛教發展歷史過程當中一個難解的現象,綜觀中國佛教,淨土一宗能否成立至今仍然聚訟紛紜,天台與禪宗由於有完整的文獻記錄,源流傳承相對清晰.至於賢首宗的歷史發展,多屬彷彿朦朧之姿,然而所幸近年許多重要史料相繼問世,明清以來賢首宗法系傳承源流幾乎可謂雲霧廓清,在此基礎之上,學界對於中國佛教宗派發展歷史進程的認識已經大幅改寫."賢首宗乘"與"賢首傳燈錄"是明清以來兩部重要的華嚴傳承史料,對認識賢首宗歷史發展過程提供一個重要線索.Taiwan resource centre for Chinese studie
Fu yang ju ji
日落 -- 撫養 -- 通車.文治平編著.Running title: 撫養劇集.Play.Wen Zhiping bian zhu.Running title: Fu yang ju ji.Ri luo -- Fu yang -- Tong che
The Molecular Structures, Expression Patterns, and Promoter Analysis of Zebrafish Troponin I Genes
抑制型肌鈣蛋白(TnnI)是位於橫紋肌細肌絲中對細肌絲的收縮提供鈣離子轉換的一個肌鈣蛋白複合體組成。臨床上在急性冠狀動脈病徵下,心臟型TnnI在脊椎動物中被當作具有高敏感性及專一性的指標。
雖然在鳥類及哺乳類中,已經確立存在三種不同異購蛋白的TnnI,分別是快肌型、心肌型、及慢肌型。但在低等脊椎動物中,對於TnnI基因的了解並未十分透徹。為了更了解在低等脊椎動物中TnnI在發育調控上所扮演的地位。首先,我們先區別了斑馬魚的TnnI基因,TnnI1及TnnI2。在脊椎動物TnnI的基因演化樹中,TnnI1及TnnI2分別相近於脊椎動物的TnnI1(slow muscle)及TnnI2(fast muscle)。在反轉錄聚合酶連鎖反應及全胚胎原位雜交法來進行觀察,結果顯示出在受精後16小時,TnnI1及TnnI2會開始表現在斑馬魚體節的部位,此外在軀幹橫切顯示出TnnI1及TnnI2是分別專一表現在慢肌及快肌,藉由這些結果來分別定義了TnnI1及TnnI2分別為斑馬魚慢肌型TnnI及快肌型TnnI。另外我們也定義了一個新的TnnI 異構型蛋白,TnnI-HC,我們觀察到TnnI-HC會表現在早期的cardiogenesis (16 hpf),且會持續在心臟專一性表現。有趣的是,在48hpf之後便會開始在頭部肌肉及心臟表現,雖然TnnI-HC較相似於慢肌型 TnnI,但卻會在心臟及頭部肌肉中有表現,這種TnnI異構型蛋白表現情形是不同於其他高等脊椎動物。所以我們利用斑馬魚基因庫來篩選TnnI-HC大約7.6-Kb的上游片段,藉由此片段可以來分析TnnI-HC在斑馬魚中的發育調控。我們選殖了兩段斑馬魚TnnI-HC上游序列1.4-Kb (-1400/+30)及intron 1 4-Kb (+2257/+6253),並分別構築了EGFP表現質體並進行顯微注射。我們發現到1.4-Kb的上游序列並沒有啟動子的能力,然而當注射intron 1 4-Kb,能觀察到intron 1 4-Kb能夠獨自驅動EGFP在心臟及軀幹中表現。由此我們假設在TnnI-HC其他上游序列中仍有其他位置能夠來加強頭部肌肉的表現及抑制體節的表現。利用deletion analysis來分析intron 1 4-Kb的序列後。我們可以定義在intron 1 (+2257/+4689)中,具有一個cis-element能夠來調控早期斑馬魚TnnI-HC基因在心臟中的表現。Troponin I (TnnI), a constituent of the troponin complex on the thin filament, provides a calcium-sensitive switch for striated muscle contraction. Cardiac TnnI is a highly sensitive and specific marker of myocardial injury in the acute coronary syndromes. Although TnnI gene has been identified in birds and mammals as it encodes the isoforms expressed in cardiac muscle, fast skeletal muscle and slow skeletal muscle, there is extremely little known about the developmental regulation of TnnI gene in the lower vertebrates. Firstly, we characterized zebrafish TnnI genes, TnnI1 and TnnI2. In the unrooted radial gene tree for TnnI genes among vertebrates, the zebrafish TnnI1 and TnnI2 genes we cloned correspond to the slow muscle TnnI1 gene and the fast muscle TnnI2 gene of other vertebrates, respectively. Secondly, reverse transcription-polymerase chain reaction (RT-PCR) and whole-mount in situ hybridization showed that TnnI1 and TnnI2 transcripts were expressed in the somites at 16 hours post-fertilization (hpf). In addition, we also demonstrated that the expression of TnnI1 and TnnI2 genes was slow- and fast-muscle-specific, respectively, indicating that zebrafish TnnI1 and TnnI2 proteins were slow-TnnI and fast -TnnI, respectively. Interestingly, we also identified a novel TnnI isoform, TnnI-HC (heart and craniofacial), which was expressed in the heart exclusively during early cardiogenesis at 16 hpf, but it was not only in the craniofacial muscle but also in the heart after 48 hpf. Thirdly, in order to go further understanding the regulatory mechanism of TnnI-HC gene, we cloned a 7.6-kb fragment containing a partial TnnI-HC genomic gene from the zebrafish genomic library. Thereafter, we subcloned an upstream 1.4-Kb (from -1400 to +30; -1400/+30) and an intron 1 4-Kb (from +2257 to +6253; +2257/+6253) and we fused them with GFP separately. After microinjection, we found that the -1.4 Kb fragment did not contain any promoter activity. However microinjection of an intron 1 fragment (4-Kb) alone without including -1.4 Kb enabled the expression of GFP in the heart and trunk muscle, but not in the craniofacial muscle. Thus, we speculate that other upstream regulatory segment might play a role in the enhancing the craniofacial muscle expression and repressing the trunk expression of TnnI-HC gene. Deletion analysis in +2257/+6253 segment, we also identified a cis-element located at +2257/+4689 within intron 1 that might control the cardiac expression of zebrafish TnnI-HC gene during early development.中 文 摘 要-------------------------------- 1
英 文 摘 要-------------------------------- 3
壹 、 前 言-------------------------------- 5
貳 、 材 料-------------------------------- 12
參 、 方 法-------------------------------- 19
肆 、 結 果-------------------------------- 35
伍 、 討 論-------------------------------- 42
陸 、 參 考 文 獻-------------------- 50
柒 、 圖 表-------------------------------- 55
捌 、 附 錄-------------------------------- 7
Matai fu yin chuan.
Special collection from London Missionary Society.; On double leaves, oriental style, in case.; Also available in an electronic version via the Internet at http://nla.gov.au/nla.gen-vn1769274.Bible. N.T.880-02 Xin yüe quan shu, Matai fu yin chua
Qu pin: fu Chuan qi pin.
曲品(卷上) / 東海鬱藍生撰, 瑯琊方諸生閱, 長洲吳梅校 -- 曲品(卷下) / 東海鬱藍生撰, 瑯琊方諸生閱, 夢鳳樓, 暖紅室刊校 -- 傳奇品(卷上) / 山陰高奕晉音氏, 長洲吳梅校 -- 傳奇品(卷下) / 山陰高奕晉音氏, 夢鳳樓, 暖紅室刊校.東海鬱藍生撰 ; 吳梅校.On double leaves, oriental style.Donghai Yu Lansheng zhuan ; Wu Mei jiao.Qu pin (juan shang) / Donghai Yu Lansheng zhuan, Langya Fang Zhusheng yue, Changzhou Wu Mei jiao -- Qu pin (juan xia) / Donghai Yu Lansheng zhuan, Langya Fang Zhusheng yue, Mengfenglou, Nuanhongshi kan jiao -- Chuan qi pin (juan shang) / Shanyin Gao Yi Jinyin shi, Changzhou Wu Mei jiao -- Chuan qi pin (juan xia) / Shanyin Gao Yi Jinyin shi, Mengfenglou, Nuanhongshi kan jiao
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