1,933 research outputs found
Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba
No full textArchaea use a variety of small basic proteins to package their DNA. One of the most widespread and highly conserved is the Alba (Sso10b) protein. Alba interacts with both DNA and RNA in vitro, and we show in the present study that it binds more tightly to dsDNA (double-stranded DNA) than to either ssDNA (single-stranded DNA) or RNA. The Alba protein is dimeric in solution, and forms distinct ordered complexes with DNA that have been visualized by electron microscopy studies; these studies suggest that, on binding dsDNA, the protein forms extended helical protein fibres. An end-to-end association of consecutive Alba dimers is suggested by the presence of a dimer-dimer interface in crystal structures of Alba from several species, and by the strong conservation of the interface residues, centred on Are and Phe(60). In the present study we map perturbation of the polypeptide backbone of Alba upon binding to DNA and RNA by NMR, and demonstrate the central role of Phe(60) in forming the dimer dimer interface. Site-directed spin labelling and pulsed ESR are used to confirm that an end-to-end, dimer dimer interaction forms in the presence of dsDNA.Peer reviewe
First person – Alba Delrio-Lorenzo
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Alba Delrio-Lorenzo is first author on ‘Sarcoplasmic reticulum Ca2+ decreases with age and correlates with the decline in muscle function in Drosophila’, published in JCS. Alba is a PhD student in the lab of Javier García-Sancho and María Teresa Alonso at the Instituto de Biología y Genética Molecular (IBGM), University of Valladolid, Spain, investigating the molecular mechanisms implicated in aging, particularly muscle aging.Peer reviewe
Suplementación de cobayos (Cavia porcellus L.) con follajes fresco de morera (Morus alba) y moringa (Moringa oleifera)
No full textA research study was conducted to evaluate guinea pig (Cavia porcellus L.) supplementation with Moringa oleifera fresh foliage or Morus alba fresh foliage and the effect on productive behavior. A total of 18 creole guinea pigs with initial body weight of 175.8 g were used in a Completely Randomized Design and with three treatments: 1) Commercial concentrate (CC) ad libitum + CT-115, 2) 70% CC + Moringa oleifera fresh foliage and 3) 70% CC + Morus alba fresh foliage with six repetitions. No signifi cant eff ects (P>0.05%) were observed between T1, T2 and T3 related with productive parameters (Body weight, gain weight and feed conversion ratio). Guinea pigs feeding with 70% CC + Moringa oleifera fresh foliage or 70% CC + Morus alba fresh foliage reduced cost production and increase fi nancial benefi t compared with guinea pigs feeding with CC ad libitum + CT-115. However, T3: 70% CC + Moringa oleifera fresh foliage showed the best financial results.Se realizó este experimento para evaluar la suplementación alimenticia para cobayos (Cavia porcellus L.) con follaje fresco de Moringa oleifere y Morus alba (Morera) y su efecto sobre el comportamiento productivo. Se utilizaron 18 cobayos mestizos de 30 días de edad, con un peso vivo inicial promedio de 175.8 gramos. Se distribuyeron en un diseño completamente al azar en tres tratamientos, con seis repeticiones. Los tratamientos evaluados fueron tres: 1) concentrado comercial (CC) a voluntad + follaje fresco de CT-115, 2) 70% CC + follaje fresco de Morera y 3) 70% CC + follaje fresco de Moringa. Los resultados indican que la alimentación de cobayos con 70% de CC y suplementados con follaje fresco de Morera o Moringa no afectan significativamente el comportamiento productivo (peso vivo, ganancia de peso y conversión alimenticia), en comparación con cobayos alimentados con CC a voluntad + CT-115. La alimentacion de cobayos con 70% de CC + follaje fresco de Moringa o 70% de CC + follaje fresco de Morera, al reducir los costos de producción, generan mayor beneficio financiero que utilizar CC a voluntad + CT-115, siendo la ración 70% de CC + follaje fresco de Moringa la que genera mayor ventaja financiera
Nucleósido 2'-desoxirribolsiltransferasa de "Bacillus psychrosaccharolyticus" CECT 4070
No full textTesis inédita de la Universidad Complutense de Madrid, Facultad de Ciencias Biológicas, Departamento de Bioquímica y Biología Molecular I, leída el 28-11-2014Sección Deptal. de Bioquímica y Biología Molecular (Biológicas)Fac. de Ciencias BiológicasTRUEunpu
A green synthesis of vidarabine 5’-monophosphate via a one-pot multienzymatic reaction catalyzed by immobilized biocatalysts
No full textIn nature, enzyme cascades can be found in many metabolic pathways. The idea of using multienzymatic
systems to mimic these processes is gaining interest for production of chemical
compounds. A type of multi-enzymatic application is the use of multiple enzymes for shifting
reaction equilibria. This strategy relies on removing intermediates, inhibitory products or byproducts,
via a second enzymatic reaction. In the context of a multi-enzymatic system, a one-pot
process uses more than one enzyme in a single reactor.1
We here describe a three-step sequential enzymatic reaction for the one-pot synthesis of vidarabine
5’-monophosphate (araA-MP), an antiviral drug, using arabinosyluracil (araU), adenine (Ade) and adenosine
triphosphate (ATP) as precursors. To this aim, three immobilized biocatalysts involved in the biosynthesis
of nucleosides and nucleotides were used: uridine phosphorylase from Clostridium perfringens (CpUP),2
a purine nucleoside phosphorylase from Aeromonas hydrophila (AhPNP),2 and deoxyadenosine kinase
from Dictyostelium discoideum (DddAK).3 Specifically, CpUP catalyzes the phosphorolysis of araU thus
generating uracil and α-D-arabinose-1-phosphate. AhPNP catalyzes the coupling between this latter
compound and Ade to form araA (vidarabine). This nucleoside becomes the substrate of DddAK which
produces the 5’-mononucleotide counterpart (araA-MP) using ATP as the phosphate donor (Scheme 1).
Reaction conditions (i.e. medium, temperature, immobilization carriers) and biocatalyst stability have
been balanced and optimized to achieve the highest productivity. Vidarabine 5’-monophosphate was
obtained in 95.5% conversion. Optimization of the purification step is in progress
The Author/Translator Interactional Process. A Case Study
No full textSee Naples and Kill (1988) is a lively and colourful novel by the con-temporary English writer, Gregory Dowling, translated into Italian in 2015.
Following the tradition of translation studies (Venuti 2000, Bass-nett 2002, Cronin 2006), this paper analyses the rewriting process of literary translation, considering in particular the fruitful but sometimes tense and even conflictual relationship between writer and translator.
The translation of the novel See Naples and Kill was an ongoing rewriting process entailing a constant dialogue between the writer and the translator. Therefore, the study aims at answering two main ques-tions: what happens if the rewriting process of translation is constant-ly questioned by the author? What happens if the author has a good mastery of the target language and s/he is her/himself a translator?
By exploring the relationship between translation and re-creation, the research focuses on the differences and similarities between the primary creation (source text) and the secondary creation (target text), and aims to verify in which way the dialogic encounter of two different personalities and cultures does not make them merge but, by retaining their own uniqueness, leads eventually to their mutually en-riching each other. A comparative analysis of the source text and the different drafts of the translated version accompanied by the author’s comments will shed light on the tense author-translator relationship in the specific case under investigation and how both actors handle this tension in order to create a new work resulting from the (dis)agreement of the two parties
A lateral flow assay for the rapid diagnosis of Mycobacterium bovis infection in wild boar
No full textThe native Eurasian wild boar (Sus scrofa ) is a reservoir of Mycobacterium bovis , the causative agent of animal tuberculosis (TB), a chronic disease in livestock, companion animals and wild mammals. Cases of M. bovis infection in wild boar or feral pig have been reported worldwide, making early detection a priority in the eradication of the disease. Point‐of‐care diagnostic tests, such as low cost lateral flow assays, provide high specificity and sensitivity and can be performed on site , an essential requirement for a rapid screening of wildlife. A lateral flow assay, LFA, (INgezim TB CROM Ab) for the detection of M. bovis ‐specific antibodies in wild boar serum and blood has been developed based on MPB83, one of the major immunogenic antigens of the bacterium. A total of 140 samples of wild boar serum, well‐characterized by Mycobacterium tuberculosis complex culture and TB compatible post‐mortem lesions, have been analysed with LFA, and results were compared with one in‐house and two commercial Enzyme‐linked Immunosorbent Assays (ELISA), INgezim TB Porcine and INgezim Tuberculosis DR. In experimental samples, the achieved values of sensitivity of the different techniques ranged from 84.3% to 92.1% and the specificity was 100% in all of them. In field animals, specificity ranged from 96% to 100%, whereas sensitivity ranged from 48% to 64% in juvenile wild boar, increasing to 93.3%–100% in adult wild boar. In particular, the total sensitivity and specificity values obtained with the new LFA were 83% and 97%, respectively, indicating that INgezim TB CROM Ab could be used as a first approach for the surveillance of TB in wild boar, with a special applicability for animal‐side testing.Part of this research was funded by the EU, Seventh Research Framework Program FP7‐KBBE‐2013‐7 under grant number nº 613799 (WildTBVac). This is a contribution to the WildDriver grant CGL2017‐89866 from MINECO and EU‐FEDER. We are very grateful to Dr. Mercedes Dominguez and her laboratory (Instituto de Salud Carlos III, Unidad de Inmunología Microbiana, Majadahonda, Spain) for their generous supply of P22 protein complex.Peer reviewe
Amorphous calcium carbonate (ACC) in fresco mural paintings
No full textA fresco painting has a calcium carbonate binder produced as result of the carbonation process of a lime paste. The reaction environmental conditions are similar to those of bio-mineral formation where an amorphous calcium carbonate (ACC) phase has been found to be the precursor of other CaCO3 polymorphs following the sequence: hydrated ACC, anhydrous ACC, vaterite, aragonite, and, finally, stable calcite. In order to determine whether ACC is also formed during the fresco drying process, as well as, the final surface calcium carbonate phases present, a series of laboratory mock-ups, replicating as close as possible each of the paint strata and submitted to the same atmospheric conditions have been designed and studied. The results indicate that the continuous supply of water and reagents by the lime and sand mortar promote the ACC formation even at the completion of water evaporation and that the high pH of the medium does not favour the formation of calcium carbonate polymorphs other than calcite. In fact, the results demonstrate that the presence of an ACC layer in the mural painting surface confirms that the original painting technique used was fresco. Finally, the presence of an ACC layer is important for stablishing adequate cleaning (the solubility of hydrated ACC is superior to those of calcite) and conservation protocols (ACC is susceptible to suffer dehydration) for the fresco paintings.The project received financial support from MINECO (Spain), grant MAT2016-77753-R and Generalitat de Catalunya, grant 2017 SGR 0042. The µSR-XRD experiments were performed at BL13 XALOC beamline at ALBA Synchrotron Facillity with the collaboration of ALBA staff (proposal 2017092488). We acknowledge the collaboration of Mireia Mestre, chief of Restoration Department of MNAC.Peer ReviewedPostprint (author's final draft
"Dolce come la vita è il mio mango!" : La Habana de Alba de Céspedes
No full textEn mi ponencia abordo la mirada de Alba de Céspedes hacia La Habana, ciudad idealizada en la distancia que la escritora italo-cubana solo conoce a raiz de la enfermedad mortal de su padre, siendo adulta ya. Para esbozar el desarrollo de esta intensa relación más literaria que cotidiana, hurgo en diferentes tipologías textuales, incluyendo "Con gran amor", novela inacabada donde la escritora quiso pintar su gran fresco cubano, donde sus peripecias familiares se entrelazan con el triunfo de la Revolución. "Dlce come la vita è il mio mango" es uno de los pregones que Alba de Céspedes cita en un artículo
Books and Monographs
No full text[ES] En este artículo se hace por un lado la recensión del libro “Inteligencia Artificial para la Supervisión de Procesos Industriales”, cuyo autor es Joseph Aguilar Martín y por otro, el resumen de cuatro tesis doctorales, relacionadas con la Automatica: “Diseño integrado de estructuras de control descentralizado en procesos y plantas químicas”, de Luis Taboada Antelo, “Identificación y optimización en tiempo real de las industrias biotecnológica y alimentaria” de Míriam R. García, “Modelling, simulation and robust control of distributed processes: application to chemical and biological systems” de Carlos Vilas Fernández, y “Estrategias de Control de Intercambiadores de Calor Termosolares” de José Domingo Álvarez Hervás.Bordons Alba, C. (2008). Libros y Monografías. Revista Iberoamericana de Automática e Informática industrial. 5(3):78-82. https://riunet.upv.es/handle/10251/145516OJS78825
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