1,132 research outputs found
Articolo di recensione a R. Perrone, "buon costume" e valori costituzionali condivisi
No full textPartendo del volume di Perrone recensito, l'articolo indaga più specificamente il ruolo delle clausole indeterminate nelle costituzioni contemporanee, soffermandosi in particolare sulla clausola del buon costume, la cui delimitazione nel diritto costituzionale, a parere dell'autrice, non può prescindere dal riferimento ad un'altra clausola indeterminata, la cui portata e sicuramente più ampia, quella cioè dell'ordine pubblico
Development of a novel Polymer-based delivery system for siRNAs to hepatocellular carcinoma cells
No full textFor Hepatocellular carcinoma (HCC) there are no effective treatments thus, the individuation of novel therapeutic approaches and molecular targets are urgent. Small interference RNA (siRNAs) are small double stranded RNAs able to induce gene silencing thus, representing an attractive therapeutic strategy. The main impediment in using siRNAs is the fast degradation rate in the biological environment. To protect siRNAs from degradation, we developed a novel delivery system based on the polymer α,β-poly-(N-2-hydroxyethyl)-D,L-aspartamide (PHEA) derivatized with diethylene triamine (DETA) linked via a polyethylene glycol (PEG) to galactose (GAL) molecules (PDPG). The GAL residue has been introduced to target to the asialoglycoprotein receptor (ASGPR), overexpressed in HCC cells.
Using a fluorescently labelled PDPG (PDPG-BodipyTRX) carrying a model siRNA, we demonstrated that the PDPG polymer can specifically deliver the siRNA to HuH7 cells, a cellular model of HCC. Notably, the elimination of the Gal residue from the PDPG (PDP) or the chemical block of ASGPR, resulted in an evident reduction of PDPG targeting effectiveness. These data have been confirmed in HuH7 constitutively expressing the enhanced green fluorescence protein (EGFP) using a functional siRNA against EGFP. Finally, PDPG delivery specificity and effectiveness was proved also in vivo in a mouse dorsal skinfold window chamber assay. Together, our data support the remarkable targeting ability of the developed PDPG.
When PDPG was loaded by siRNAs targeting HCC proliferative factors (eEF1A1, eEF1A2 and E2F1), we observed a clear decrease of HuH7 cell vitality and cell number. These phenomena were paralleled by the reduction of targeted mRNAs and relative proteins levels. Interestingly, siRNAs-PDP, lacking the Gal residue was poorly effective in reducing HuH7 vitality and number. Finally, in vivo experiments performed in a subcutaneous xenograft mouse model of HCC, revealed the ability of siRNAs-PDGP to reduce HCC tumor growth compared to controls. Notably, PDPG did not show significant polymer-related toxic effects as evaluated by monitoring animal weight and the levels of markers of organs function.
In conclusion, our study demonstrates the priceless potentials of PDPG as a novel delivery system for siRNA paving the way for innovative anti-HCC treatments
Takotsubo Cardiomyopathy: Overview
No full textTakotsubo Cardiomyopathy: Overview Eduardo Bossone, Gianluigi Savarese, Francesco Ferrara, Rodolfo Citro, Susanna Mosca, Francesca Musella, Giuseppe Limongelli, Roberto Manfredini, Antonio Cittadini, and Pasquale Perrone Filardi Takotsubo cardiomyopathy (TTC) is a unique acute syndrome characterized by transient left ventricular systolic dysfunction in the absence of significant coronary artery disease, occurring mostly in postmenopausal women after emotional and/or physical stress. Given the nonspecific symptoms and signs, a high clinical index of suspicion is necessary to detect the disease in different clinical settings and scenarios. Noninvasive multimodality imaging may be useful to distinguish this cardiomyopathy from other acute cardiac and thoracic diseases. Coronary angiography remains, however, mandatory to differentiate TTC from acute coronary syndromes. This article reviews the clinical features and management of TTC and some new insights
The cultural landscape between the permanence of the value of identity and the establishment of an active social role. The Complex of Maxentius on the Appia Antica.
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La pianificazione di area vasta tra promesse energetiche e scenari di sviluppo. Riflessioni sul caso Basilicata
No full textIn Basilicata lo sfruttamento di giacimenti di petrolio da oltre due decenni non ha frenato l’esodo della popolazione, producendo poca occupazione e forte esposizione a rischi ambientali e di declino socio-culturale. Incertezza economica e inefficacia della pianificazione territoriale richiedono il rilancio del “governo del territorio”, attraverso una maggiore condivisione delle scelte di sviluppo da parte delle comunità e un diverso approccio politico e culturale a livello regionale e nazionale.For over two decades, the exploitation of oil fields in Basilicata has not slowed down the exodus of people, creating a small number of new jobs and high exposure to environmental and socio-cultural decline risks. Economic uncertainty and the ineffectiveness of territorial planning require the relaunching of “territorial management”, through a greater sharing of communities development choices and a different political and cultural approach at regional and national levels
Exploring Approaches to Define Cultural Ecosystem Services in Planning Support System
No full textCultural ecosystem services (CES) play a crucial role in enhancing human well-being by providing intangible benefits such as spiritual enrichment, aesthetic enjoyment, and cultural identity. However, defining and categorizing CES presents a significant challenge due to their subjective nature and multifaceted dimensions. This paper critically examines various approaches adopted in defining and conceptualizing CES within the realm of ecosystem services research. Through a review of existing literature, we explore the diversity of perspectives and methodologies used to identify, assess, and classify cultural services provided by ecosystems. Various approaches exist for spatial identification of CES, ranging from territorial indicator-based methods to philosophical theories. However, literature analysis reveals the difficulty in universally defining evaluation methods applicable to all cases. While macrolevel guidelines aid in recognizing potential CES, contextual analysis of structural elements is necessary. Integrating a system for assessing immaterial values can track both material and non-material benefits of CES within the territory. This suggests a context-based approach for both identification and evaluation of CES, essential for effective planning processes. By synthesizing diverse conceptualizations and methodological approaches, this paper aims to contribute to a deeper comprehension of the complexities inherent in defining and valuing cultural ecosystem services, ultimately fostering more effective conservation and sustainable management strategies
Women Academics in England, 1870-1930
No full textBased on the author's dissertation, this article traces the development of the academic profession for women in England in the late 19th and early 20th centuries, focusing on women at Oxford, Cambridge and London universities. Unlike in the United States, where women's role in higher education expanded and then retracted during this period, British women slowly and steadily made inroads into this male-dominated profession.Peer reviewe
Catholic Women’s Colleges in the United States: An Archival, Bibliographic and Historical Survey
No full textBrief history of Catholic women's colleges in the United States and bibliographic essay on published and archival sources
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