120 research outputs found
Procedimenti prospettici cinquecenteschi
No full textSeminario su “Prospettive” organizzato dal Prof. Maurizio Unali dell’Università degli Studi “G. D’Annunzio”, Facoltà di Architettura di Pescara.
Pescara, 7 novembre 2000.
L’intervento di Marco Fasolo dal titolo: “Procedimenti prospettici cinquecenteschi” ha inteso mettere a confronto le costruzioni prospettiche dei Maestri del Cinquecento, F. Brunelleschi, L. B. Alberti e Piero della Francesca. Da quest’ analisi è scaturita l’osservazione che alla base dei procedimenti costruttivi c’è sempre l’idea di usare rette a 45° col quadro per mettere in prospettiva un punto di una perpendicolare al quadro, cioè per misurarla. Pertanto, anche in questo momento storico, fondamentale per lo sviluppo della teoria prospettica, lo spunto iniziale d’origine è sempre spaziale, geometrico e non teorico
Il contorno apparente di Piero della Francesca. Indagine e riflessioni
No full textIn Piero della Francesca coesistono due anime quella di pittore e quella di matematico, l’una inscindibile dall’altra. Il seguente articolo intende approfondire il teorema “Nel termine asignato, col dato puncto, proportionalmente la testa degradare” contenuto nel suo celebre trattato De prospectiva pingendi nel quale il rapporto e il connubio tra arte e scienza trova una efficace applicazione: la realizzazione in prospettiva di una testa. In particolare viene formulata un’ipotesi di ricostruzione del procedimento utilizzato da Piero e vengono presentate alcune considerazioni sul problema della determinazione dell’immagine del contorno apparente di una superficie
"Vincenzo Fasolo. Primato del Disegno e dell’Ambientismo"
No full textThe essay wants to give a historical-critical prophile’s of Vincenzo Fasolo (Split 1885 - Rome 1969), as part of his work as an architect, architectural historian and professor of Storia e stili dell’architettura (History of Architecture and Architectural styles from 1925 to 1961). The text includes a cognitive process of drawings, who represented the guidelines to understand the theories of the thinning out of the building and residential devolution discussed in Rome in the early decades of the XX century. These theories are characterized by continual reference to the history of architecture and architectural styles and they are referenced to the respect of buildings environmental and local art, T the same time they represented an alternative to the uniformity that characterized the constructive operations take out after the Italian unification in the city of Rome. The Fasolo’s teaching and professional experience is defined, through the theories and knowledge of the architectural history, also conveyed by a unique versatility for the drafting, seen as an essential tool for analysis and description. The graphic capabilities define the design path of Vincenzo Fasolo, in respect to urban design to minimize the difference between the historic city centre and suburbs, recognizing in the study of architectural styles (in their various manifestation, linked to local art and time) the centre of a culture historiographical and projectual, aimed at establishing an evolutionary code for the design
SINEUPs, a new class of antisense long non-coding RNAs that enhance synthesis of target proteins in cells: molecular mechanisms and applications
No full textThanks to continuous advances in sequencing technologies, we know that a huge
number of non-coding RNAs are transcribed from mammalian genomes. Of these,
long non-coding RNAs (lncRNAs) represent the widest and most heterogeneous
class. An increasing number of studies are unveiling lncRNA functions, supporting
their active role in regulating gene expression. Regardless of lncRNAs specific
functional features, their organization into discrete domains seems to represent a
common denominator. Through such domains lncRNAs can recruit and coordinate
the activity of multiple effectors, thus working as “flexible modular scaffolds”. This
model has globally driven towards the quest for regulatory elements within
lncRNAs, with a special attention on functional cues deriving from RNA folding.
Since transposable elements (TEs) represent 40% of nucleotides of lncRNA
sequences, they have been proposed as candidate functional modules. Carrieri and colleagues recently reported that an embedded inverted SINEB2 element
acts as a functional domain in antisense (AS) Uchl1, an AS lncRNA able to increase
translation of partially-overlapping protein-coding sense Uchl1 mRNA. AS Uchl1
regulatory properties depend on two RNA domains. A 5' overlapping sequence to the
sense transcript is the Binding Domain (BD) and drives specificity of action. An
embedded inverted SINEB2 element functions as Effector Domain (ED) conferring
translational activation power. AS Uchl1 is the representative member of a new class
of lncRNAs, named SINEUPs, as they rely on a SINEB2 element to UP-regulate
translation. AS Uchl1 activity can be transferred to a synthetic construct by
manipulating the AS sequence in the BD, suggesting the potential use of AS Uchl1-
derived synthetic SINEUPs as tools to increase translation of selected targets.
This work was the first example of a specific biological function assigned to an
embedded TE leading to the hypothesis that embedded TEs provide functional
modules to lncRNAs.
A major limit to the application of SINEUPs is represented by the poor knowledge of
the basic mechanisms underlying the biological activity of the ED. A crucial
challenge becomes the identification of secondary structures that may confer
characteristic protein binding properties. Protein partners would modulate SINEUPs action and contribute to achieve specific functional outputs.
In this thesis, I focus on understanding the molecular basis of SINEUPs activity in
cells and I discuss the potential applications of synthetic SINEUPs as translation
enhancers.
First, I investigated the structural basis for translation activation mediated by the ED
of SINEUPs. I pointed out that specific structural regions, containing a short terminal
hairpin, are involved in the ability of natural and synthetic SINEUPs to increase
translation of target mRNAs.
Next, I identified protein partners modulating the activity of SINEUPs in cells. I
found that AS Uchl1 interacts with the interleukin enhancer-binding factor 3 (ILF3)
and that the presence of the inverted SINEB2 favors binding in vivo. In particular, I
demonstrated that the AS Uchl1-embedded TEs, inverted SINEB2 and Alu, direct
AS Uchl1 localization to ILF3-containing complexes, thus contributing to AS Uchl1
bias towards nuclear localization. I thus suggest that nuclear retention could
represent a possible mechanism regulating SINEUP activity. I also validated the scalability of synthetic SINEUPs as tools to increase protein
synthesis of targets of choice. I showed that SINEUP technology can be adapted to a
broader number of targets, with interesting potential applications in different fields,
from biotechnology to therapy. SINEUPs function in an array of cell lines and can be
efficiently directed toward N-terminally tagged proteins. Their biological activity is
retained in a miniaturized version within the range of small RNAs length. Their
modular structure can be exploited to successfully design synthetic SINEUPs against
selected endogenous targets, supporting their efficacy as tools to modulate gene
expression in vitro and in vivo. Hence, I propose SINEUPs as versatile tools to
enhance translation of mRNAs of choice
Un museo per la collezione di antichità dei Torlonia: due progetti di Vincenzo Fasolo nell’immediato dopoguerra.
No full textPost 1940 the architect Vincenzo Fasolo found himself drawing up plans for a “Museum for ancient artefacts” within Villa Torlonia in Roma. The idea was to transfer the Torlonia Collection from the rooms it was housed in in Via della Lungara. The blueprints for his work are in the Fasolo Archives kept in the Archivio Storico Capitolino. Fasolo had two differing architectural approaches to the job. They illustrate the stylistic and museographic changes going on in said debate during the years after the Second World War. Neither of the two proposals ever saw the light of day. However, the planned project goes to show that Fasolo’s professional relationship with the Torlonia family didn’t come to an end with the Casina delle Civette in 1917, or the project for the Palazzo già Giraud in Via della Conciliazione, but instead carried on after the wa
Memoria della città nel secondo dopoguerra. La ricostruzione come interpretazione dell’antico nei progetti di Vincenzo Fasolo
No full textL’attenzione alla città, la logica di dialogo tra il vecchio ed il nuovo e le trasformazioni urbane sono temi cardine dell’operato di Vincenzo Fasolo. A seguito delle distruzioni della Seconda Guerra Mondiale, i due progetti non realizzati per Faenza (1945-1954) e per l’area di Ponte Vecchio a Firenze (1946-1947) mostrano una riflessione sulla complessa realtà di
stratificazione urbana ed apportano un inedito contributo al dibattito sull’intervento di restauro postbellico di brani di città.The focus on the city, the interrelation between old and new, the urban transformations, are all main principles of the projects by Vincenzo Fasolo. After the destructions of the Second World War, the unrealised projects on Faenza (1945-1954) and on the area of Ponte Vecchio in Florence (1946-1947) show a broad assessment of the complex situation of urban stratification, developing a completely new contribution on the post-war restoration debate
Excursus storico sui Fondamenti Scientifici della Rappresentazione
No full textSeminario dal titolo: Introduzione alla Geometria Descrittiva, organizzato dal Dottorato di Ricerca in Scienze della Rappresentazione e del Rilievo. Marco Fasolo è intervenuto con una comunicazione sui seguenti temi: Illustrazione dello sviluppo storico dei fondamenti geometrici della rappresentazione: dalle regole geometriche della visione fondate sull’ottica di Euclide nel 300 a.C. nel suo Optikè e sulle opere di Tolomeo alle rappresentazioni prospettiche nelle pitture pompeiane. Il periodo fecondo del Rinascimento per la codifica della prospettiva con le figure e le relative opere di Brunelleschi, Leon Battista Alberti, Piero della Francesca, Leonardo da Vinci, di Durer, di Palladio e del Serlio, prologo alla ormai prossima scientifizzazione della disciplina da parte di autori, protesi alla ricerca di sopportare questi studi con basi matematiche rigorose, ne sono fulgidi esempi le opere di Federico Commandino e del suo allievo Guidobaldo del Monte. Nel settecento si accentua la frattura tra gli studiosi "scientifici" e quelli "letterari" con il conseguente disinteresse verso i lavori reciproci. Gaspard Monge risolve questo dualismo, all'apparenza insanabile, fondando una nuova disciplina, la Geometria Descrittiva, definita da lui stesso come: "l'art de représenter sur une feuille de dessin qui n'a que deux dimensions, les corps de l'espace qui en ont trois qui son susceptibles d'une définition rigoreuse". Nell’ottocento viene codificato il metodo di rappresentazione assonometrico grazie agli studi di Tramontini, Fasish, Shlomilch.
Giunti verso il termine di questo viaggio nella storia, è doveroso ricordare anche la fondamentale figura di Guglielmo Fiedler che con il suo Trattato di Geometria Descrittiva (1874) rende la prospettiva come metodo autonomo di rappresentazione.
Questo è il cammino della Scienza della rappresentazione che approda fino ai giorni nostri con i maestri del nostro recente passato e con quelli di oggi.
Pertanto la geometria si pone come strumento essenziale per il concepimento di forme e movimenti nello spazio tridimensionale ed accettato il passaggio obbligato della percezione, cadono le riserve sulla legittimità della rappresentazione, sulla sua insufficienza, sulla sua erroneità e su tutto il bagaglio di contestazioni approssimative del suo valore conoscitivo, documentativo e critico. Il primato del disegno ne esce rafforzato; così pure l'indispensabilità della geometria e l'utilità degli studi che su di essa e tramite essa possono condursi. Mi riferisco in particolare all’attuale nuovo metodo di rappresentazione, la modellazione informatica che deve, e spetta a noi studiosi di geometria descrittiva, trovare la continuità tra le nuove tecnologie e la storia, la tradizione della rappresentazione
Vincenzo Fasolo (1885 Spalato-1969 Roma). Il Palazzetto del Governatorato a Ostia Lido e la sua decorazione.
No full textVincenzo Fasolo (1885 Split-1969 Rome). The Governor’s Palace in Ostia Lido and its decoration · This study deals with a particular administrative building erected by the architect Vincenzo Fasolo during the Fascist period (1928-1932) in Ostia Lido, at that time the new suburban beaching resort of Rome. Focus point of this
examination is the decoration in sgraffito or graffito technique developed on the
outer and inner walls of the courtyard of the building. The use of this methods finds probably its origin in Fasolo’s background, since he was a Dalmatian native. The decorator Umberto Calzolari, executer of this work, has been able to create, in a late Liberty and imaginative style, a combination of mythological elements with contemporary symbols. An absolute unusual oeuvre for a municipal building
LA CONTESA SULLA RICOSTRUZIONE DI FAENZA NEL PROGETTO DI VINCENZO FASOLO. RESTAURO E RICOSTRUZIONE POSTBELLICA FRA CONTINUITÀ E NUOVE ISTANZE
No full textThe paper focuses on the reconstruction projects for the city and the belfry of Faenza, after the
destruction of the Second World War. Both the urban and the architectural plans were carried out
by the “roman group” led by Vincenzo Fasolo, who adhered to Giovannoni’s scientific restoration
and to the thinking of “ambientismo”. This case study highlights the evolution of the restoration
discipline in Italy and Fasolo’s struggles in understanding and applying the new critical demands
of conservation
The Influence of Insecure Romantic Attachment on Generativity
No full textExisting research concerning the development of generativity, the capacity to provide care and guidance to future generations, has primarily focused on the role of sociodemographic variables. However, one important feature that might be of importance for the study of generativity is insecure romantic attachment, given its influence on how people establish relationships with others. Still, the role of the two dimensions of insecure romantic attachment (i.e., anxiety and avoidance) on the most studied aspects of generativity (i.e., generative concern and generative acts) remains largely underexplored. Therefore, the present study investigates what contributes to generativity by exploring the role of sociodemographic features (i.e., age, gender, having children, years of education) and above all the two dimensions of insecure romantic attachment on generative concern and acts. A sample of 427 adults (age range: 25–65 years old) completed an online survey including romantic attachment and generativity measures. Afterwards, correlational and regression analyses were conducted to explore the data. Results showed that years of education positively predicted generative concern, whereas both anxiety and avoidance negatively predicted it. Yet, no sociodemographic feature nor dimension of insecure romantic attachment predicted generative acts. Thus, insecure romantic attachment could be a useful key to understanding generative concern. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature
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