92 research outputs found
Streptococcus equi subsp. zooepidemicus epidemiological and genomic findings of an emerging pathogen in central Italy
No full textStreptococcus equi subsp. zooepidemicus: epidemiological and genomic findings of an emerging pathogen in Central Italy
No full textAntimicrobial resistance trends in equine population of Central Italy for Streptococcus equi subsp. zooepidemicus
No full text
Outbreak of porcine epidemic diarrhoea virus (PEDV) in Abruzzi region, central-Italy
No full textHere we report and characterize a porcine epidemic diarrhea (PED) outbreak which occurred in a swine fattening farm in the province of Teramo, Abruzzi region (central Italy), in January 2016. PED virus (PEDV) identification was determined by real-time RT-PCR performed on RNAs purified from fecal samples collected from two symptomatic pigs. Whole genome sequence (PEDV 1842/2016) was also obtained by next generation sequencing straight from RNA purified from one fecal sample. Genome comparison with extant global PEDV strains revealed a high nucleotide identity with recently reported European and American S-INDEL PEDVs. Efficient sequencing, share of genomic data combined with the implementation of epidemiological tools would be the ideal approach for study and analysis of transboundary infectious diseases as PED
Mapping the surveillance activities for food-hazards across Europe
No full textAim: MATRIX is part of the One Health European Joint Programme aiming at the implementation of One Health Surveillance
(OHS), through the strengthening of the whole process of surveillance, and the implementation of guidelines for OHS
improvement. The Project relies on a problem-based approach and focuses on four hazard-tracks: Salmonella, Listeria,
Campylobacter and an emerging threat. As Work Package 2, a mapping of the surveillance chain across all sectors for each
hazard-track has been performed from late 2020 to early 2021.
Methods: Per each hazard-track, including Hepatitis E selected as the emerging pathogen of interest, a specific food chain
was selected to explore in detail the different realities. Twelve online questionnaires were implemented based on the ”farm to
fork” approach for the three sectors (animal health, food safety and public health) and each hazard-food chain combination.
Surveillance activities in place were investigated in at least two countries for each combination.
Results: Salmonella was investigated in humans and pork meat food chain; Listeria in humans and dairy products;
Campylobacter in humans and poultry meat; Hepatitis E, in humans and wild boar meat. Answers were categorized in order to be graphically displayed, in: events, actors, data, metadata, event producing data, identified data sources, and sharing potential. Answers of these categories represent the start to identificate cross-sectorial linkages across surveillance chains.
Conclusions: The mapping of surveillance activities in place was the first step in the identification of best-practices for multisectorial collaboration. The following phase is the identification of outputs that could be shared for One Health oriented
decision-making
Interactive Static Software Performance Analysis in the IDE
No full textDetecting performance issues due to suboptimal code during the development process can be a daunting task, especially when it comes to localizing them after noticing performance degradation after deployment. Static analysis has the potential to provide early feedback on performance problems to developers without having to run profilers with expensive (and often unavailable) performance tests. We develop a VSCode tool that integrates the static performance analysis results from Infer via code annotations and decorations (surfacing complexity analysis results in context) and side panel views showing details and overviews (enabling explainability of the results). Additionally, we design our system for interactivity to allow for more responsiveness to code changes as they happen. We evaluate the efficacy of our tool by measuring the overhead that the static performance analysis integration introduces in the development workflow. Further, we report on a case study that illustrates how our system can be used to reason about software performance in the context of a real performance bug in the ElasticSearch open-source project.Demo video: https://www.youtube.com/watch?v=-GqPb-YZMOs Repository: https://github.com/ipa-lab/vscode-infer-performanceAccepted author manuscriptSoftware Engineerin
THE CLINICAL ROLE OF CONTRAST-ENHANCED ULTRASOUND IN THE EVALUATION OF RENAL ARTERY STENOSIS AND SUPERIORITY IN THE RENAL ARTERY STENOSIS DIAGNOSIS COMPARED TO TRADITIONAL ECHO-COLOR-DOPPLER FLOW IMAGING
No full textCorrelación Cito-Histológica entre Diagnóstico de Citología Rápida y Biopsia Diferida de Ganglios Centinelas Axilares y Tejido Mamario, entre los años 2009 a 2011 en el Hospital Dr. Gustavo Fricke, Viña del Mar
No full textTesis (Tecnólogo Médico, especialidad en Morfofisopatología y Citodiagnóstico)La Citología Rápida es una evaluación microscópica de células obtenidas mediante el raspado o impronta de piezas quirúrgicas. En esta evaluación microscópica se estudian las características morfológicas de las células. La citología rápida es considerada desde hace mucho tiempo como una herramienta muy importante en el apoyo al diagnóstico, no sólo por su simplicidad y bajo costo, sino también por el gran detalle citológico que presenta.
En la presente investigación se trabajó con Citologías Rápidas o también llamadas Citologías Intraoperatorias, en el cual se realizó una comparación de los resultados de la técnica de Citología Rápida con los resultados de la Biopsia Diferida, con la finalidad de obtener el porcentaje de correlación existente entre ambas técnicas en muestras de Ganglios Centinelas Axilares y Tejido mamario, obtenidos de la Unidad de Anatomía Patológica del Hospital Dr. Gustavo Fricke de Viña del Mar.
Se estudiaron los casos en los que no existió una Correlación Cito-Histológica, evaluando los factores técnicos como causales de una no correlación con el diagnóstico Histopatológico. Posteriormente se realizó la estandarización de la técnica de Citología Rápida con el fin de disminuir la falta de Correlación Cito-Histológica según lo observado en el procedimiento realizado en la Unidad de Anatomía Patológica, con esto se espera mejorar la calidad diagnóstica de los exámenes.
Se obtuvo un porcentaje de correlación de un 96,6%, con lo cual se corrobora el porcentaje esperado según a la revisión de estudios previos, uno de ellos realizado el año 2005 en el Servicio de Anatomía Patológica del Hospital Clínico de la Universidad de Chile, el cual indicaba un porcentaje entre 90%-95%
Identification of the Novel <i>Streptococcus equi</i> subsp. <i>zooepidemicus</i> Sequence Type 525 in Donkeys of Abruzzo Region, Italy
No full textStreptococcus equi sub. zooepidemicus (SEZ) is described as a commensal bacterium of several animal species, including humans. Growing evidence supports the potential role of SEZ in the onset and progression of severe clinical manifestations of diseases in horses and other animals. In the present communication, we describe the diagnostic procedure applied to characterize the streptococcal infections caused by a novel SEZ sequence type (ST525) in donkeys raised on a farm in Abruzzo, Italy. The diagnostic process began with anamnesis and anatomopathological analysis, which revealed a severe bacterial suppurative bronchopneumonia associated with systemic vascular damage and haemorrhages. Then, SEZ infection was confirmed by applying an integrative diagnostic strategy that included standard bacterial isolation techniques, analytical tools for bacteria identification (MALDI-TOF MS), and molecular analysis (qPCR). Furthermore, the application of the whole-genome sequencing approach helped us to identify the bacterial strains and the virulence factors involved in animal diseases. The novel SEZ-ST525 was identified in two cases of the disease. This new sequence type was isolated from the lung, liver, and spleen in Case 1, and from retropharyngeal lymph nodes in Case 2. Moreover, the presence of the virulence gene mf2, a virulence factor carried by prophages in Streptococcus pyogenes, was also found for the first time in an SEZ strain. The results of the present study highlight the need to apply an integrated diagnostic approach for the identification and tracking of pathogenic strains of SEZ, shedding new light on the re-evaluation of these bacteria as a causative agent of disease in animals and humans
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