334 research outputs found
Preparation of herpes simplex virus type 1 (HSV-1)-based amplicon vectors
No full textAmplicon vectors, or amplicons, are defective, helper-dependent, herpes simplex virus type 1 (HSV-1)-based vectors. The main interest of amplicons as gene transfer tools stems from the fact that the genomes of these vectors do not carry protein-encoding viral sequences. Consequently, they are completely safe for the host and nontoxic for the infected cells. Moreover, the complete absence of virus genes provides a genomic space authorizing a very large payload, enough to accommodate foreign DNA sequences up to almost 150-kbp, the size of the HSV-1 genome. This transgene capacity can be used to deliver complete gene loci, including introns and exons, as well as long regulatory sequences conferring tissue-specific expression or stable maintenance of the transgene in proliferating cells. For many years the development of these vectors and their application in gene transfer experiments was hindered by the presence of contaminating toxic helper virus particles in the vector stocks. In recent years, however, two different methodologies have been developed that allow generating amplicon stocks either completely free of helper particles or only faintly contaminated with fully defective helper particles. This chapter describes these two methodologies
Multifluorescence live analysis of herpes simplex virus type-1 replication
No full textThe possibility to label specific viral and cellular structures with live cell markers such as autofluorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows for monitoring virus replication in a spatial and temporal fashion. Here, we describe the multifluorescent live analysis of the multicompartment Herpes Simplex Virus Type-1 (HSV-1) by live cell confocal laser scanning microscopy
Religion, rap and the crisis of black leadership Cornel West
No full textBill Moyers interviews activist, author and teacher Cornel West about the state of African-American society, its struggle for liberty, and its living, vibrant traditions.Host, Bill Moyers. Photographer, Joel Shapiro ; editor, Michael Collins ; music, Michael Bacon
Lowell Lecture Series: Cornel West: Race Matters (videorecording), 1996
No full textOn October 2, 1996, the Lowell Lecture Committee at Suffolk University hosted Dr. Cornel West, distinguished author, professor, and lecturer, to speak in the series Stemming the Tide of Violence in the United States and Beyond.
Dr. West\u27s talk, entitled Race Matters after his award-winning book, was delivered at the C. Walsh Theatre, and included a Q&A session with the audience, followed by a brief reception. These lectures were funded by a grant from the Lowell Institute and were open to the public.https://dc.suffolk.edu/su-av/1028/thumbnail.jp
Herpes simplex virus type 1 (HSV-1)-derived recombinant vectors for gene transfer and gene therapy
No full textHerpes simplex virus type 1 (HSV-1 ) is a human pathogen whose lifestyle is based on a long-term dual interaction with the infected host, being able to establish both lytic and latent infections. The virus genome is a 153-kilobase pair (kbp) double-stranded DNA molecule encoding more than 80 genes. The interest of HSV-1 as gene transfer vector stems from its ability to infect many different cell types, both quiescent and proliferating cells, the very high packaging capacity of the virus capsid, the outstanding neurotropic adaptations that this virus has evolved, and the fact that it never integrates into the cellular chromosomes, thus avoiding the risk of insertional mutagenesis. Two types of vectors can be derived from HSV-1, recombinant vectors and amplicon vectors, and different methodologies have been developed to prepare large stocks of each type of vector. This chapter summarizes the approach most commonly used to prepare recombinant HSV-1 vectors through homologous recombination, either in eukaryotic cells or in bacteria
Herpes Simplex Virus Growth, Preparation, and Assay
No full textIn order to study the biology of herpes simplex virus or to use it as a vector in gene therapy, it is necessary to grow the virus and to prepare virus stocks. Many different protocols are available from different research groups working with herpes simplex virus type 1 or 2 (HSV-1 or HSV-2). This chapter describes the procedures used in our laboratory
2019 MLK Keynote Address: Cornel West
No full text2019 MLK Series Keynote Cornel West lecture Wednesday, January 23, 2019 at 7:00pm, RISD Auditorium,17 Canal Walk, Providence RI. The Center for Social Equity and Inclusion (SEI) welcomes 2019 MLK Series Keynote speaker Cornel West, a professor, philosopher, author and activist. West is professor of the practice of public philosophy at Harvard University and a professor emeritus at Princeton University. He has also taught at Union Theological Seminary, Yale, and the University of Paris and has written and edited many books about race and democracy, including Race Matters, Democracy Matters and the memoir Brother West: Living and Loving Out Loud. His most recent book, Black Prophetic Fire, offers an unflinching look at visionary 19th- and 20th-century African American leaders and their legacies. West is a frequent guest on the Bill Maher Show, CNN, C-Span and Democracy Now. He made his film debut in the Matrix and was the commentator (with Ken Wilbur) on the official trilogy released in 2004. He also has appeared in more than 25 documentaries and films, including Examined Life, Call & Response, Sidewalk and Stand.
In communicating with a vast variety of publics, West is committed to keeping alive Martin Luther King, Jr.\u27s legacy of truth-telling and bearing witness to love and justice
Cornel West
No full textWMPG celebrates the lives of Black men and women throughout the month of February. Doctor Cornel West, born in 1953 Tulsa, OK, is an author, professor, Ivy League scholar, philosopher, political activist, actor, and political commentator. He has written twenty books and focuses on race, gender, and class in American society.https://digitalcommons.usm.maine.edu/wmpg_bhm/1006/thumbnail.jp
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