202 research outputs found
DQA1*0102 DQB1*0602 haplotype distinguishes coeliac disease and its complications from gluten unrelated enteropathies
No full textBackground: Duodenal villous atrophy is due not only to coeliac disease and its complications but also to other rare enteropathies unrelated to gluten consumption, defined as noncoeliac enteropathies. The diagnosis of noncoeliac enteropathies remains challenging, and HLA typing has been widely used to exclude coeliac disease if DQ2 and DQ8 alleles are absent. However, the frequency of the various HLA alleles in noncoeliac enteropathies is still unknown. Aims: To describe the HLA genetic profile of patients affected by noncoeliac enteropathies who have been evaluated at our centres between 2000 and 2021, and to investigate the diagnostic role of HLA typing. Methods: Genomic DNA was collected from 44 Italian and 19 British adult patients with noncoeliac enteropathies. Patient genotypes were compared with those of healthy Italian and British populations obtained from HLA bone marrow donors' banks. In addition, genotypes were also compared with those of patients with coeliac disease and complicated coeliac disease. Results: Both in the Italian and in the British group, the DQA1*0102 DQB1*0602 haplotype and related alleles occurred significantly more frequently in patients with noncoeliac enteropathies compared to coeliac disease and complicated coeliac disease. Conclusions: Together with negative HLA-DQ2 and DQ8 haplotypes, the DQA1*0102 DQB1*0602 haplotype can be used to guide the differential diagnosis between coeliac disease and noncoeliac enteropathies
A 3-D data smoothing algorithm
No full textThe algorithm presented in this report is designed for use in smoothing 3-D data at NUWES. It uses fourth-order sequential differences to screen the data for outliers and then a special form of least-squares smoothing is performed to select an appropriate low-order polynomial and fit it to seven-pont data segments. (Author)N0025381WR70012NAResearch and Engineering Department, Naval Undersea Warfare Engineering Station, Keyport, Washingtonhttp://archive.org/details/3ddatasmoothinga00tys
Chronicles of Oklahoma
No full textArticle narrates how the author met Reverend Willis F. Folsom and describes his thoughts about the man. Reverend Folsom received his education from Bloomfield Academy. Later, he served as a reverend within the Choctaw Nation
The Graded School Speller: Part One
No full textThis is the first of several sequential books to help students with spelling and vocabulary. Five fables are presented in curious fashion. The five are AD (8-9) and TH, The Boys and the Frogs, FC, and DM (16-23). Each is presented in twenty-four disparate sentences on two pages. The book is surprising to me because it seems to belong to a series. Ginn and Company published several different sets of books for grade-school pupils, but Spaulding and Miller did not seem to be a part of their team. On the other hand, Spaulding published a series of grade-school books for Aldine in 1913. His co-author was Catherine Bryce. Is the series referred to here perhaps only a series of three spellers? This book has seen plenty of wear. One clever student has created a window on the first sheet that says Come in. Open the window, and you will see the face drawn on the title-page! The book once belonged to a young John in Las Vegas, New Mexico.This is a hardbound book (hard cover)Frank E. Spaulding and William D. Mille
Quagliana v. Exquisite Home Builders, Inc. : Brief of Respondent
No full textIN THE LAW LIBRARY SUPREME COURTS 6 ,975 OF THE STATE OF UTi4MHAM Y0UN6 UNIVERSIIY J7 Reuben Clark Law School JOSEPH M. QUAGLIANA and PAULA L. QUAGLIANA, Plaintiffs and Appellants, vs. EXQUISITE HOME BUILDERS, INC., a n d ALLAN KRUCKENBERG, GARY MARGETTS, dba K M DESIGN, Defendants and Respondents. Case No. 13723 BRIEF OF RESPONDENTS Appeal From a Judgment of the District Court of Salt Lake County Honorable Bryant H. Croft, Judge WAYNE A. ASHWORTH DONALD SAWAYA 2805 South State Street Salt Lake City, Utah 84115 Attorneys for Defendants and Respondents Kruckenberg. Margetts, and K M Design BRYCE E. ROE ROE AND FOWLER 340 East Fourth South Salt Lake City, Utah 84111 jf— Attorneys for Plaintiffs and Appellants*** ORVAL C. HARRISON » 15 East 400 South Salt Lake City, Utah 84111 Attorney for Defendant and Respondent,. Exquisite Home Builders, Inc. LE
Targets of transcription inhibition by the antibiotics lipiarmycin, GE23077, and salinamide
No full textThe antibiotics lipiarmycin (Lpm), GE23077 (GE), and salinamide (Sal) function by inhibiting bacterial RNA polymerase (RNAP). In this work, the targets (and mechanisms) of transcription inhibition by Lpm, GE, and Sal are identified and characterized through a combination of genetic, biochemical, and structural approaches. Each of these compounds functions through a different target on the enzyme that does not significantly overlap the targets of other bacterial RNAP inhibitors. Elucidation of these targets may prove useful for antibacterial drug discovery and design. To define the functional target of Lpm, we isolated and sequenced 160 Lpm-resistant mutants. In the structure of RNAP, sites of substitutions conferring Lpm-resistance cluster to define the “Lpm target,” which includes residues in the RNAP switch-region, as well as one wall of the RNA exit channel. Biochemical experiments show that Lpm inhibits the RNAP-DNA interaction, and appears to function by trapping the RNAP clamp in a fully-to-partially closed state. To define the functional target of GE, we isolated and sequenced 35 GE-resistant mutants. In the structure of RNAP, sites of substitutions conferring GE-resistance cluster to define the “GE target,” which includes residues in the RNAP active-center subregions, the “β D2-loop” and the “link region.” Biochemical experiments reveal that GE inhibits nucleotide addition during transcription initiation, after open complex formation, but prior to phosphodiester bond formation. The crystal structure of RNAP in complex with GE confirms that GE binds to the GE target, and indicates that GE functions by precluding the binding of NTP substrates to the RNAP active-center "i site" and "i+1 site.” To define the functional target of Sal, we isolated and sequenced 47 Sal-resistant mutants. In the structure of RNAP, sites of substitutions conferring Sal-resistance cluster to define the “Sal target,” which includes residues in the RNAP active-center subregions: the “bridge-helix N-terminal hinge” (BH-HN), the “F-loop,” and the “link region.” Biochemical experiments reveal that Sal inhibits nucleotide addition during both transcription initiation and elongation. The crystal structure of RNAP in complex with Sal confirms that Sal binds to the Sal target, and suggests that Sal functions by trapping the BH-HN in a straight (unbent) conformation.Ph. D.Includes bibliographical referencesby David D. Dege
Chronicles of Oklahoma
No full textArticle narrates how the author met Major General Edward Hatch and his first impressions of the soldier. The author met the general while he was stationed as an agent within Indian Territory
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