1,720,972 research outputs found
Dection of hepatitis A Virus (HAV) in shellfish (Mytilus galloprovincialis) using RT-PCR
No full textA PCR assay for the detection of hepatitis A virus (HAV) in shellfish is described. The procedure involves the concentration of viral particles with the use of polyethylene glycol (PEG), followed by viral RNA extraction and purification with oligo(dT) cellulose. Reverse transcriptase-PCR detection was accomplished in a single step with the use of primers specific for the VP3-VP1 region of the genome. The procedure detected one 50% tissue culture infective dose (0.6 PFU) per 25 g of shellfish homogenate. Heminested PCR was then carried out to verify the specificity of the PCR products. The method was used to detect HAV in shellfish samples from EU categories B and C and to evaluate the quality of shellfish in routine monitoring for HAV in view of the relevant public health implications of this foodborne disease
Duplex polymerase chain reaction (D-PCR) for detection of pork meat in horse meat fresh sausages from Italian retail sources
No full textSpecies identification in meat products represents an important subject in the field of modern food control according to the
European Union, which has implemented a set of very strict procedures to label food. Thus, specific, sensitive and easy analytical
methods for the species detection of food are necessary in order to verify the compliance with labelling requirements. A PCR-based
assay for the detection of pork meat in horse fresh sausages was optimised and it was used to evaluate the presence of fraudulently
added pork meat. The developed assay showed the presence of pork meat in 6/30 and the total absence of horse meat in 1/30 of the
analyzed horse sausage samples
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