1,185 research outputs found
Annette Harvey Diary, 1906-1910
No full textAnnette Harvey, of Arkansas, West Virginia, and Ohio, recounts events of her daily life in this 'Line a Day' diary. She was the daughter of William Hope Harvey, aka 'Coin' Harvey, a well-known businessman, politician, author and founder of the resort of Monte Ne and the Ozark Association. Annette's brief entries record visits, housework, dances, parties, a train trip to New York, weather, church services and socials over a 5 year period, 1906-1910. Addresses and miscellaneous thoughts, quotations, poems, are recorded at the end of the volume. A photograph of her home made in 1906 is tipped in at the front of the diary
The Nup358-RanGAP complex is required for efficient importin alpha/beta-dependent nuclear import
No full textIn vertebrate cells, the nucleoporin Nup358/RanBP2 is a major component of the filaments that emanate from the nuclear pore complex into the cytoplasm. Nup358 forms a complex with SUMOylated RanGAP1, the GTPase activating protein for Ran. RanGAP1 plays a pivotal role in the establishment of a RanGTP gradient across the nuclear envelope and, hence, in the majority of nucleocytoplasmic transport pathways. Here, we investigate the roles of the Nup358-RanGAP1 complex and of soluble RanGAP1 in nuclear protein transport, combining in vivo and in vitro approaches. Depletion of Nup358 by RNA interference led to a clear reduction of importin alpha/beta-dependent nuclear import of various reporter proteins. In vitro, transport could be partially restored by the addition of importin beta, RanBP1, and/or RanGAP1 to the transport reaction. In intact Nup358-depleted cells, overexpression of importin beta strongly stimulated nuclear import, demonstrating that the transport receptor is the most rate-limiting factor at reduced Nup358-concentrations. As an alternative approach, we used antibody-inhibition experiments. Antibodies against RanGAP1 inhibited the enzymatic activity of soluble and nuclear pore-associated RanGAP1, as well as nuclear import and export. Although export could be fully restored by soluble RanGAP, import was only partially rescued. Together, these data suggest a dual function of the Nup358-RanGAP1 complex as a coordinator of importin beta recycling and reformation of novel import complexes.Deutsche Forschungsgemeinschaft [KE 660/5-1, SFB523, TP18
Interview with Annette Lareau
No full textAnnette Lareau is the Stanley I. Sheerr Professor in the Department of Sociology at the University of Pennsylvania. She is the author of Unequal Childhoods: Class, Race, and Family Life (University of California Press). Unequal Childhoods won the best book award from three sections of the American Sociological Association: Sociology of the Family, Sociology of Children and Youth, and Sociology of Culture (co-winner)
Charakterisierung des RanGAP1-RanBP2 Komplexes in Mitose
No full textDer RanGAP1-RanBP2 Komplex stellt ein faszinierendes makromolekulares Gebilde dar, welches mindestens zwei enzymatische Aktivitäten umfasst. Zum einen beinhaltet der Komplex die GTP-Hydrolyse aktivierende Funktion, die RanGAP1 zusammen mit RanBP2 ausübt, zum anderen besitzt RanBP2 zusammen mit Ubc9 Sumo-konjugierende Aktivität. Gemeinsam sind diese Proteine essentielle Regulatoren des nukleo-zytoplasmatischen Transportes in Interphasezellen, und spielen des Weiteren eine wichtige, bislang jedoch kaum verstandene Rolle für die Funktion der Kinetochore in Mitose.Um den RanGAP1-RanBP2 Komplex spezifisch in mitotischen Zellen näher zu untersuchen, habe ich spezifisch in Mitose nach Interaktionspartnern gesucht. Dies führte zur Identifikation des Kernexportrezeptors Crm1 und der GTPase Ran als stabile Komponenten in einem Komplex mit RanGAP1, RanBP2 und Ubc9 in mitotischen Zellen. Zusätzlich schien dieser Komplex zahlreiche weitere Proteine in substöchiometrischen Mengen zu enthalten. Diese könnten beispielsweise NES-enthaltende Interaktionspartner von Crm1 und/oder Substrate für RanBP2-abhängige Sumoylierung sein. Da bisher RanBP2-abhängige Sumo-Substrate weitgehend unbekannt sind, habe ich eine Strategie entwickelt, um sumoylierte Proteine aus immungereinigtem RanGAP1-RanBP2 Komplex anzureichern. Dies ermöglichte die massenspektrometrische Identifizierung von ungefähr 90 potentiellen Sumo-Substraten, die spezifisch in mitotischen RanGAP1 Komplexen angereichert waren; 6 dieser Substrate wurden zur weiteren Charakterisierung ausgewä! hlt. Alle Kandidaten assoziierten mit mitotischen RanGAP1 Komplexen (Topo II alpha, TACC2, CKAP-5, Plk1, USP7, PIAS1). Die meisten davon konnten entweder in vitro mit rekombinanten Faktoren (TACC2, Plk1) oder als endogene Proteine, die mit mitotischem RanGAP1-RanBP2 Komplex als Quelle der Sumo E3 Ligase-Aktivität assoziiert waren (TopoII alpha, Plk1, USP7), sumoyliert werden. Auffällig war die Co-Reinigung der Sumo E3 Ligase PIAS1 mit RanGAP1 aus mitotischen Zellen; diese wurde ebenfalls effizient sumoyliert. Weitere Analysen deuteten darauf hin, dass RanGAP1 in einem Komplex mit PIAS1 enthalten ist, der sich vom RanGAP1-RanBP2 Komplex unterscheidet.In einem Nebenprojekt konnte ich zeigen, dass das Sumo-konjugierende Enzym Ubc9 in Zellen an Lysin 14 sumoyliert werden kann. Dieser Befund war wichtig, um eine biochemische Studie von Knipscheer et al. zu ergänzen, die einen neuen Mechanismus der Selektion von Sumo-Substraten identifizierte; diese Ergebnisse flossen in die Veröffentlichung Knipscheer, Flotho, Klug et al. (2008) Mol Cell ein.The RanGAP1-RanBP2 complex represents a fascinating macromolecular assembly comprising at least two enzymatic activities. On one hand, it harbors the GTP hydrolysis activating function of RanGAP1 together with RanBP2, and on the other hand, RanBP2 in concert with Ubc9 contains Sumo conjugating activity. Together, these proteins are not only crucial regulators of nucleocytoplasmic transport in interphase cells but they also play an important yet ill-defined role in kinetochore function during mitosis.To gain insight into the RanGAP1-RanBP2 complex specifically in mitotic cells, I searched for mitosis-specific interaction partners. This led to the identification of the nuclear export receptor Crm1 and the GTPase Ran as stable components in complex with RanGAP1, RanBP2 and Ubc9 in mitotic cells. In addition, the complex seemed to contain many different proteins at substochiometric levels. These could, for example, be NES containing Crm1 interactors and/or targets for RanBP2 dependent sumoylation. As RanBP2 dependent Sumo targets are largely unknown, I devised a strategy to enrich sumoylated proteins from immunoprecipitated RanGAP1-RanBP2 complexes. This allowed mass-spectrometric identification of 90 putative Sumo substrates specifically enriched in mitotic RanGAP1 complexes; 6 of these were selected for further validation. All candidates associated with mitotic RanGAP1 complexes (Topo II alpha, TACC2, CKAP-5, Plk1, USP7, PIAS1), and most of these could be sumoylated in vitro with recombinant factors (TACC2, Plk1) or as proteins associated with mitotic RanGAP1-RanBP2 complexes as source of Sumo E3 ligase activity (TopoII alpha, Plk1, USP7). Strikingly, the Sumo E3 ligase PIAS1 also co-purified with RanGAP1 from mitotic cells and was efficiently sumoylated in these experiments. Further analysis suggested that mitotic RanGAP1 is present in a complex with PIAS1 distinct from the RanGAP1-RanBP2 complex.In a side project, I could show that the Sumo conjugating enzyme Ubc9 is sumoylated on lysine 14 in cells. This finding was crucial to supplement a biochemical study by Knipscheer et al. that identified a novel mechanism for Sumo substrate selection and contributed to the publication Knipscheer, Flotho, Klug et al. (2008) Mol Cell
Interview with Annette J. Smith
No full textInterview in seven sessions, December 2010 to January 2011 with Annette J. Smith, visiting professor of French at Caltech from 1970 to 1982, appointed associate professor with tenure in 1982, promoted to professor of French in 1985, and Professor of Literature emeritus since 1993.
Family history, childhood and education in Algiers, Algeria. Family history and background of late husband, Caltech Professor of Literature David R. Smith (1960-1990). Bachelor’s degree in Classics (1948) from Sorbonne in Paris. Attended the School of Professors of French Abroad at the Sorbonne and taught at the University of Wales in Swansea. Master’s degree in English. Marriage to D. Smith and move to the United States.
Teaches at Scripps College and Claremont Men’s College [now Claremont McKenna College], where she had tenure position. Caltech hires D. Smith as professor and A. Smith as lecturer in French language. D. Smith as Joseph Conrad scholar. Doctorate degree (1964) and dissertation on author Nicole Védrès. D. Smith made Master of Student Houses (1969-1975); life in Virginia Steele Scott house. Descriptions of faculty and atmosphere within Division of Humanities and Social Sciences (HSS), beginning when Hallett Smith was chair. Friendship with Max and Manny Delbrück. Cultural life at Caltech; D. Smith brings poets, actors, directors and musicians to campus. Life as professor’s spouse and efforts to improve working conditions and salaries for female staff. Sexual discrimination in HSS and support for Jenijoy La Belle. History and founding of Baxter Art Gallery (1970), significant exhibitions organized by D. Smith, closing of Baxter Art Gallery (1985). Important relationships with Caltech professors, postdocs and staff: R. Sperry, R. Feynman, A. Hibbs, J. and F. Audouze, D. and C. Cesarsky, J.-P. Bibring, and N. and C. Corngold.
Elevated to associate professor (1982). Literature courses she taught and impressions of students. Two books accepted for publication: one on Arthur de Gobineau and translation of poems by Aimé Césaire. Explanation of racial theories of Gobineau and discussion of his fiction; impact of Gobineau’s racist writings and theories, including appropriation by Nazis. Discussion of Darwinism. Comments about translating poetry and working with poet Clayton Eshleman on four books of Césaire’s poetry. Description of Césaire’s life and politics and his importance as a leader and author. Reads her translations of Césaire’s poems.
Impressions of foreign language study at Caltech and further descriptions of HSS, including some unfortunate hires and tension in the division. D. Smith’s illness and death. Teaching in Papeete, Tahiti, 1990-1991. Circular nature of her life and work. Purchase of land and building of second home in Point Dume, Malibu, (1980-1981) and celebratory party there. Expressions of gratitude for Caltech and its brilliant scientists and community
The censor without, the censor within: the resistance of Johnstone’s improv to the social and political pressures of 1950s Britain
No full textKeith Johnstone's improv, popularly known through the Theatresports format, was forged in the cultural and historical context of 1950s Britain. In this paper I will argue that Johnstone's incarnation of theatrical improvisation was defined by its reaction to the normalising forces exerted by the social elite upon the broader population and by civilised society upon the individual.
Johnstone's improv was a reaction against the Lord Chamberlain’s power to censor the British stage and a challenge to the internalised 'censor' British society of the time implanted in the minds of his students, stunting their creative imaginations. Johnstone borrowed elements of professional wrestling to break down the regimented conventions of the theatre space and enliven the spectator-performer relationship. As well as echoing Roland Barthes’ idealistic analysis of professional wrestling (Barthes, 1984: n.p.), Johnstone’s improv shares Barthes’ critique of the authority of the author and allows meaning to be generated out of the encounter between performers and spectators in the instant of the performance’s emergence. Through these processes, Johnstone’s improv defies the censor without (The Lord Chamberlain) by rooting out the censor within (the socially learnt inhibitions to the creative imagination).
By delineating the political and social pressures at play in the historical context of 1950s Britain and the ways that the stylistic conventions of Johnstone's improv resist and subvert these forces, I will demonstrate the emancipatory power latent in this mode of popular performance. This is a particularly timely analysis given the increasing authority of free market economics to dictate what appears on contemporary British stages, and the internalised censor that panoptical CCTV and social media is implanting within the minds of British citizens today
Integrative Differentielle Relaxation (IDR) in der Psychotherapie – Möglichkeiten und Grenzen im dyadischen Setting
No full textIn der vorliegenden Arbeit wird der Ansatz der Integrativen Differentiellen Relaxation (IDR) unter Einbezug spezifischer Konzepte der Integrativen Therapie und aktueller stresstheoretischer Aspekte vorgestellt. Es erfolgt eine Standortbestimmung des IDR-Ansatzes bezüglich der allgemein üblichen Entspannungsverfahren wie bspw. Autogenes Training und Progressive Muskel-Relaxation. Die Spezifika des Ansatzes werden dargestellt und es wird der Frage von Möglichkeiten und Grenzen der Einbettung in das dyadische psychotherapeutische Setting nachgegangen. Hierbei wird insbesondere auf die Übertragungs- und Gegenübertragungsaspekte fokussiert, die es zu beachten gilt, wenn „übende Elemente“ in das psychotherapeutische Setting einbezogen werden. Die theoretischen Ausführungen werden durch ein Fallbeispiel aus der Praxis der Autorin ergänzt.This text discusses Integrative Differential Relaxation (IDR) in relation to specific Integrative Therapy and stress-theoretical aspects. It follows a localization of the IDR approach within traditional relaxation techniques such as autogenic training and Progressive Muscle Relaxation (PMR). The discussion outlines the specifics of IDR and addresses its options and limits within the dyadic psychotherapeutic settings. In particular it focuses on conceptual questions of transference and countertransference relative to exercising elements as part of psychotherapy.In conclusion the author applies the theory to a case study.https://www.fpi-publikation.de/polyloge/10-2008-frankenstein-anft-annette-integrative-differentielle-relaxation-in-der-psychotherapie/peerReviewedpublishedVersio
FTAA: What's in It for the South?
No full textNot everyone in the Americas thinks that negotiating an FTAA is desirable. Some argue that the timing of the negotiations is being set by the agenda of the developed countries, particularly the US, and not that of the rest of the region. Others say that negotiating tariff reductions will do little to increase exports. The argument is that non-tariff barriers to trade must be part of the package, or the whole idea is a waste of time. These are just some of the opinions coming from the South. Interestingly, a number of these ideas are coming from Brazil, the hemisphere's most populous country after the US, and clearly a leader in the region. Presidential elections in Brazil took place in the fall of 2002 just prior to an FTAA Ministerial in Quito. In the lead up to the election, the FTAA positions of the opposition candidates, including the eventual winner, were much more protectionist than that of the outgoing government. If the protectionism carries through to official government policy, then the FTAA process will be much more difficult. However, this might just have been electoral talk. This paper will attempt to sort out truth from rhetoric.Brazil, non-tariff barriers, FTAA, South, International Relations/Trade,
The student welfare role of the classroom teacher
No full textDeposited with permission of the author. © 2002 Annette WilkinsonThis research project explores the welfare role of the classroom teacher in the secondary setting. (For complete abstract open document
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