52 research outputs found
Mecanismos moleculares involucrados en la regionalización naso-temporal de la vesícula óptica en pez cebra
No full textTesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 26-07-2018Esta tesis tiene embargado el acceso al texto completo hasta el 26-01-2020Esta tesis ha sido financiada a través de un proyecto de la Comisión Europea (CIG321788) de la Dra. Florencia Cavodeassi, y de varios proyectos del Ministerio de Economía y Competitividad concedidos a la Dra. Florencia Cavodeassi (BFU2014-55918-P y BFU2011-24701) y a la Dra. Paola Bovolenta Nicolao (BFU2013-43213-P)
Adhesive/repulsive codes in vertebrate forebrain morphogenesis
No full text© 2014 by the authors. The last fifteen years have seen the identification of some of the mechanisms involved in anterior neural plate specification, patterning, and morphogenesis, which constitute the first stages in the formation of the forebrain. These studies have provided us with a glimpse into the molecular mechanisms that drive the development of an embryonic structure, and have resulted in the realization that cell segregation in the anterior neural plate is essential for the accurate progression of forebrain morphogenesis. This review summarizes the latest advances in our understanding of mechanisms of cell segregation during forebrain development, with and emphasis on the impact of this process on the morphogenesis of one of the anterior neural plate derivatives, the eyes.My research is funded by a National Project Grant of the Spanish Government (BFU2011-24701) and the EU (PCIG11-GA-2012-321788), and by an Institutional Grant from the Fundación Ramón Areces to the Centro de Biología Molecular Severo OchoaWe acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI)Peer Reviewe
Dynamic Tissue Rearrangements during Vertebrate Eye Morphogenesis: Insights from Fish Models
No full textOver the last thirty years, fish models, such as the zebrafish and medaka, have become essential to pursue developmental studies and model human disease. Community efforts have led to the generation of wide collections of mutants, a complete sequence of their genomes, and the development of sophisticated genetic tools, enabling the manipulation of gene activity and labelling and tracking of specific groups of cells during embryonic development. When combined with the accessibility and optical clarity of fish embryos, these approaches have made of them an unbeatable model to monitor developmental processes in vivo and in real time. Over the last few years, live-imaging studies in fish have provided fascinating insights into tissue morphogenesis and organogenesis. This review will illustrate the advantages of fish models to pursue morphogenetic studies by highlighting the findings that, in the last decade, have transformed our understanding of eye morphogenesis
Brain regionalization: Of signaling centers and boundaries
No full textOur knowledge of the general mechanisms controlling the formation of the vertebrate central nervous system has advanced tremendously in the last decade. Here, we discuss the impact of the combined use of cell manipulation, in vivo imaging and genetics in the zebrafish on recent progress in understanding how signaling processes progressively control regionalization of the central nervous system. We highlight the unresolved issues and speculate upon the fundamental role the zebrafish will continue having in answering them. (c) 2011 Wiley Periodicals, Inc. Develop Neurobiol 72: 218233, 201
Funcion de los genes Iroquis en el desarrollo de la cabeza de Drosophila
No full textCentro de Informacion y Documentacion Cientifica (CINDOC). C/Joaquin Costa, 22. 28002 Madrid. SPAIN / CINDOC - Centro de Informaciòn y Documentaciòn CientìficaSIGLEESSpai
Función de los genes Iroquis en el desarrollo de la cabeza de Drosophila
No full textTesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 25-02-200
Brain regionalization: of signaling centers and boundaries
No full textOur knowledge of the general mechanisms controlling the formation of the vertebrate central nervous system has advanced tremendously in the last decade. Here, we discuss the impact of the combined use of cell manipulation, in vivo imaging and genetics in the zebrafish on recent progress in understanding how signaling processes progressively control regionalization of the central nervous system. We highlight the unresolved issues and speculate upon the fundamental role the zebrafish will continue having in answering them.Peer Reviewe
Early stages of retinal development depend on Sec13 function
No full textER-to-Golgi transport of proteins destined for the extracellular space or intracellular compartments depends on the COPII vesicle coat and is constitutive in all translationally active cells. Nevertheless, there is emerging evidence that this process is regulated on a cell- and tissue-specific basis, which means that components of the COPII coat will be of differential importance to certain cell types. The COPII coat consists of an inner layer, Sec23/24 and an outer shell, Sec13/31. We have shown previously that knock-down of Sec13 results in concomitant loss of Sec31. In zebrafish and cultured human cells this leads to impaired trafficking of large cargo, namely procollagens, and is causative for defects in craniofacial and gut development. It is now widely accepted that the outer COPII coat is key to the architecture and stability of ER export vesicles containing large, unusual cargo proteins. Here, we investigate zebrafish eye development following Sec13 depletion. We find that photoreceptors degenerate or fail to develop from the onset. Impaired collagen trafficking from the retinal pigment epithelium and defects in overall retinal lamination also seen in Sec13-depleted zebrafish might have been caused by increased apoptosis and reduced topical proliferation in the retina. Our data show that the outer layer of the COPII coat is also necessary for the transport of large amounts of cargo proteins, in this case rhodopsin, rather than just large cargo as previously thought
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