170,144 research outputs found
L'educatore e le immagini del suo ruolo
No full textL'articolo si riferisce alla ricerca sull'idea di cultura e di immagine professionale condotta tra un gruppo di matricole del corso di laurea per Educatore dell'università di Padova, al termine dei primi tre anni di attivazione del nuovo corso universitario. L'articolo esplora le ragioni che spingono gli studenti ad immatricolarsi in questo curricolo di studio ed esamina quale immagine di educatore emerge quando viene chiesto agli studenti di descrivere tale figura attraverso una rappresentazione metaforic
Cultura e identità professionale dell'educatore all'inizio del percorso universitario
No full textI percorsi universitari che preparano a svolgere le professioni educative (l'insegnante, il formatore, l'educatore) si rivolgono a giovani iscritti che, già al momento dell'immatricolazione al primo anno di corso di laurea, appaiono 'formati' da idee, vissuti, atteggiamenti, modelli educativi pre-esistenti alla loro decisione accademica. Gli studenti che li scelgono, infatti, sono stati tutti oggetto dell'azione educativa, intenzionale o non intenzionale, di genitori, insegnanti, mentori, educatori di vario tipo, con ruoli sia espliciti che impliciti; in molti casi, essi hanno vissuto nel riflesso di familiari, amici o conoscenti impegnati in queste professioni, e ciò li ha portati ad apprezzare, o meno, alcuni aspetti dell'essere educatori. In qualche caso, essi stessi hanno svolto attività educative nell'ambito del volontariato o del lavoro temporaneo. Lo scopo di questo lavoro è quello di capire se ed in quale misura tali fattori possono contribuire ad orientare la scelta verso percorsi universitari che formino alla professione educativa, nonché a veicolare una (corretta) rappresentazione della figura professionale. Il presente studio considera le modalità con le quali un gruppo di 167 iscritti al primo anno del corso di laurea in Educatore (negli indirizzi sia di ambito sanitario che di ambito sociale) si rappresenta la professione di educatore a partire da atteggiamenti, motivazioni, esperienze che confluiscono nell'interpretazione della futura identità professionale e nell'identificazione delle caratteristiche di tale identità. Si tratta di uno studio di tipo esplorativo che suggerisce ai docenti alcune indicazioni di riferimento per svolgere con maggior consapevolezza il proprio compito formativo, anche per meglio supportare la preparazione dei futuri educatori
Heparin-induced structural and functional alterations of bovine trypsin.
No full textTo investigate the mechanism whereby heparin can modulate the activity of serine proteinases, bovine trypsin was chosen as reference and treated with heparin at 10, 100 and 200 μg/ml, in buffer solvents, with and without incubation at 37°C. Heparin caused rapid, buffer- and pH-dependent decrease in trypsin solubility due to the generation of insoluble fragments from proteinase. Desalting treatments variously restored solubility by removing insoluble material. UV absorption and fluorescence emission spectra revealed significant heparin-induced conformational alterations in the trypsin molecule, the maximal effect being apparent at a proteinase-to-heparin molar ratio ranging from 1.6 to 1.0. The involvement of the catalytic sites of trypsin by heparin was further confirmed by the significant reduction in the difference absorption spectra of proflavine. Both proteolytic and esterolytic activities of trypsin were shown to be markedly decreased by heparin, especially after 5 h incubation at 37°C. However, when the proteolytic and esterolytic activities of trypsin were measured on fresh solutions not submitted to desalting treatments, variable activation instead of inhibition of both activities was observed in the presence of heparin, this effect waning spontaneously in time or after desalting treatment. The paradoxical increase in functional activities was not inhibited by soybean trypsin inhibitor and was accompanied by denaturation and fragmentation of the proteinase as demonstrated by spectroscopic analyses and SDS-PAGE of fresh solutions. The results obtained indicate that heparin causes a rapid, time- and temperature-dependent conformational alteration of trypsin with irreversible denaturation and degradation of the proteinase. The underlying mechanism appears to be heparin-catalyzed oxidative degradation of trypsin due to liberation of oxygen radicals which are also responsible for the temporary increase in catalytic functions
Biphasic pattern of heparin-induced oxidative degradation of trypsin in the presence of glucose.
No full textThe present work tested the hypothesis that the oxidative effects of heparin on trypsin may be modified by the addition of another reducing substance. Glucose was chosen as reducing sugar and used at a concentration (15 mM) and an incubation time (5 h at 37 degrees C) with trypsin which may cause only modest oxidative modifications, thus resembling what happens in vivo in the presence of slight, long-lasting hyperglycaemia. The effects of 10, 100 and 200 micrograms/mL standard heparin on both structural and functional properties of glucose-treated bovine trypsin were measured by UV and fluorescence emission spectra and by the proteolytic and esterolytic activities of trypsin on two different substrates. Radical formation was measured by the reduction of nitroblue tetrazolium and acetylated cytochrome c. The influence of incubation and desalting of solutions were also evaluated. In the absence of heparin but in the presence of glucose, no gross structural alteration of trypsin was observed, nor was any fragmentation of the enzyme visible in the electrophoretic pattern. However, despite the fact that soluble free radicals were not detectable, glucose did induce the formation of reactive species linked to trypsin itself, as confirmed by the findings of increased reduction of nitroblue tetrazolium by fresh undesalted, and incubated desalted glucose-treated trypsin solutions. Heparin, mostly at 10 micrograms/mL (which caused an increase of 9.64 nmol reduced cytochrome c/mg protein per min) significantly changed both the nature and concentration of the radicals which were formed. Heparin-induced oxygen radical production was rapid in onset, and markedly modified the structure and function of the enzyme which in time underwent complete inactivation and fragmentation. At both 100 and 200 micrograms/mL, heparin appeared to slow the oxidative process, as spectroscopic analysis and electrophoretic pattern showed less profound structural modifications of trypsin. Overall results show that heparin-induced oxidation of trypsin follows a biphasic, concentration-dependent pattern in the presence of another reducing substance such as glucose. Low heparin concentrations enhance the oxidative potential of glucose, whereas higher concentrations antagonize it
Studio di microRNA come molecole utilizzabili in approcci di teranostica
No full textIl progetto prevede lo studio di microRNA come molecole utilizzabili in approcci di teranostica. In particolare l’identificazione di molecole a microRNA a) utili per identificare procedure di autoemotrasfusione negli atleti; b) utili per poter diagnosticare la presenza di patologie tumorali mediante analisi di biopsie liquide e nuove piattaforme tecnologiche; c) come molecole target in nuove strategie terapeutiche per malattie genetiche rare quali la fibrosi cistica, la beta-talassemia e la sindrome Shwachman Diamond
MicroRNA profiling in biopsie liquide di pazienti con Sindrome di ShwachmanDiamond: identificazione delle basi molecolari della patologia e di nuovi markers prognostici.
No full textShwachmanDiamond (SDS) è una malattia rara ereditaria autosomica recessiva caratterizzata da mutazioni del gene SDBS e da insufficienze d’organo multiple. Il 15-20% dei pazienti sviluppa sindrome mielodisplastica, con rischio di leucemia mieloide
acuta. La terapia attuale consiste in trapianto di midollo e supplementazione con enzimi pancreatici. Lo scopo del progetto è valutare, mediante microarrays e droplet digital PCR quantitativa (ddPCR), il contenuto dei microRNA presenti (a) nel plasma di pazienti SDS, (b) in linee cellulari linfoblastoidi da essi derivate, (c) secreti nel terreno di coltura (microvescicole/esosomi). Il materiale biologico (da circa 10 pazienti SDS ed altrettanti soggetti sani) è già stato ottenuto dal Centro Regionale Specializzato per la Sindrome di ShwachmanDiamon, Az. Osp. Univ. Integrata di Verona (Dott. Cipolli,Bezzerri, Sorio) (1). L’identificazione di microRNA differenzialmente espressi in pazienti SDS rispetto a soggetti sani permetterà di identificare possibili meccanismi molecolari utili per nuovi approcci terapeutici. L’integrazione dei risultati con dati clinici (includendo età, sesso, stadio della patologia e risposta alla terapia) consentirà di identificare microRNA con valore prognostico rispetto all’evolversi della patologia verso quadri clinici più severi (es leucemia) e alla risposta a specifici trattamenti terapeutici
Progettazione e sviluppo di scaffolds naturali per la rigenerazione ossea
No full textFor many years, the regenerative medicine has been using the biometarials like grafts to promote healing of bone tissue.
A heterologous graft should not evoke any inflammatory response where it is inserted and its antigenicity is eliminated with a decellularization technique .
The aim of this study was to provide a protocol creating a bovine xenograft which easily employed in medicine field for repairing bone defects. For this purpose, the decellularization protocols were applied on 26 rats' femur by means of freezing /disfreezing. The effect of the decellularization in vitro was tested by means of MTT and the light microscope (SEM).
The bovine xenograft obtained was inserted surgically in maxillary sinus of 30 sheeps to test the ability of regeneration of biomaterial in vivo.
The surgical intervention was carried out bilaterally: one side was experimental side (xenograft) and the other side was control side ( Bio-oss®,Geistlich Pharma AG Wohlhusen, Switzerland).
The study subjects were divided in 3 groups .Each group was consisting of 10 animals. They were euthanazied on 15, 30, 60 days from the surgical intervention, respectively.
Collected bone samples were decalcified, fixed and colored with hematoxylin-eosin and subsequently, they were evaluated under optical microscope.
The MTT tests showed that the duration of freezing at -20°C for 7 days and e -80°C for another 7 days, followed by disfreezing through thermal shock was more efficient for correct decellularization of the bovine extracellular matrix.
The RT-PCR results indicate that the bovine xenograft has the optimum osteoinductive competence.
In vivo tests showed that the biometarial already present for 15 days, has promoted the capacity of osteoconductive and osteoinductive with absence of inflammatory response.
The “gold standard” in medicine manifests the excellent osteoconductive and osteoinductive capacity at 60 days.
In conclusion, it can be confirmed that the protocol of decellularization by means of freezing /disfreezing, allows producing an ideal bone replacement for regeneration of bone defects in medicine field
Effects of intact and reduced reducing termini in heparin on structural and functional modifications of trypsin.
No full textThe identity and professional culture of the Educator at the beginning of the university path in Italy
No full textTo work as an educator in Italy has required a specific university degree for only a few years now. This change has institutionalized the educational path regarding some factual knowledge and skills. However, the profession of educator also relates to an experiential culture and to personal motivations affecting and sustaining each individual’s choice of the profession. This paper examines the ideas of professional identity held by a group of Italian students at the beginning of their college career. The sample includes two groups at the University of Padua, one group learning to teach in social work, one group learning to teach in health care. During the first three years of the new academic requirement, first-year students in both groups were qualitatively and quantitatively surveyed. In particular the study explores the reasons students orient towards these curricola, and the distinctive elements, competences, experiences and values they include in their image of an educator. Statistical multidimensional methods of data analysis are used
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