1,848,602 research outputs found
Oral History Interview with L. C. Finger, June 14, 2004
No full textThe National Museum of the Pacific War presents an oral interview with L.C. Finger. Finger was born in Garner, Texas. Drafted into the Army in 1943, he was sent to Camp Wolters, Texas for three weeks of basic training. He was then sent to Camp Mackall, North Carolina where he joined the 11th Airborne Division, volunteering for parachute training. He made five practice jumps before deploying overseas. Arriving at Leyte in June 1944 they made three practice jumps and conducted routine patrols. He recalls in December a Japanese force came out of the jungles and attacked an airfield and an engineering group killing many Americans. Elements of Finger’s division searched and were successful in finding and destroying the enemy force. That evening the Japanese dropped paratroopers to take the local airfield and he tells of seeing them come down, highlighted by white parachutes, enabling the Americans to kill many of them as they landed. The division retook the airfield the next day. While participating in the action, Finger was seriously wounded by a Japanese sniper. He was taken to an aid station and then to a field hospital where doctors removed his right leg. He was taken to Biak, New Guinea and stayed in a hospital area set up in a Catholic church. He was then sent back to the United States where he was sent to a military hospital in Temple, Texas. He was discharged in September 1945
Setting the scene : background and overview of regulatory reform in the transport sector
Full text linkThis book concerns the regulation of transport within a European context, covering air, rail, road passenger and freight, urban public transport, inland waterways, short sea shipping and intermodal transport. All these sectors have experienced substantial changes over the last three decades and this book aims to present the main such changes and their impacts. Over this period, and since the publication of the first European Commission (EC) Transport White Paper in 1992 (European Commission, 1992), important reforms of the transport sectors have been introduced in individual member states and supported by various EC initiatives, including implementation of several Directives and a number of Green and White Papers, most recently in 2011 (European Commission, 2011). Even though this has not been an objective of the Commission, there have been changes in ownership structures moving away from significant state intervention towards more autonomous entities and even private sector involvement for both transport infrastructure and operations. Some progress has also been achieved in terms of competition for transport operations, facilitated amongst others through third-party access rights to the markets
Finger-Positional Change in Three Zinc Finger Protein Sp1: Influence of Terminal Finger in DNA Recognition<sup>†</sup>
No full textThe connection of functional modules is effective for the design of DNA binding molecules
with the desired sequence specificity. C2H2-type zinc finger proteins have a tandemly repeated array structure
consisting of independent finger modules and are expected to recognize any DNA sequences by permutation,
multi-connection, and the substitution of various sets of zinc fingers. To investigate the effects of the
replacement of the terminal finger on the DNA recognition by other fingers, we have constructed the
three zinc finger peptides with finger substitution at the N- or C-terminus, Sp1(zf223), Sp1(zf323), and
Sp1(zf321). From the results of gel mobility shift assays, each mutant peptide binds preferentially to the
target sequence that is predicted if the fingers act in a modular fashion. The methylation interference
analyses demonstrate that in the cases of the N-terminal finger substitution mutants, Sp1(zf223) and Sp1(zf323), the N-terminal finger recognizes bases to different extents from that of the wild-type peptide,
Sp1(zf123). Of special interest is the fact that the N-terminal finger of the C-terminal finger substitution
mutant, Sp1(zf321), shows a distinct base recognition from those of Sp1(zf123) and Sp1(zf323). DNase
I footprinting analyses indicate that the C-terminal finger (active finger) induces a conformational change
in the DNA in the region for the binding of the N-terminal finger (passive finger). The present results
strongly suggest that the extent of base recognition of the N-terminal finger is dominated by the binding
of the C-terminal finger. This information provides an important clue for the creation of a zinc finger
peptide with the desired specificity, which is applicable to the design of novel drugs and biological tools
Zinc finger recombinases with adaptable DNA sequence specificity
Full text linkSite-specific recombinases have become essential tools in genetics and molecular biology for the precise excision or integration of DNA sequences. However, their utility is currently limited to circumstances where the sites recognized by the recombinase enzyme have been introduced into the DNA being manipulated, or natural 'pseudosites' are already present. Many new applications would become feasible if recombinase activity could be targeted to chosen sequences in natural genomic DNA. Here we demonstrate efficient site-specific recombination at several sequences taken from a 1.9 kilobasepair locus of biotechnological interest (in the bovine beta-casein gene), mediated by zinc finger recombinases (ZFRs), chimaeric enzymes with linked zinc finger (DNA recognition) and recombinase (catalytic) domains. In the "Z-sites" tested here, 22 bp casein gene sequences are flanked by 9 bp motifs recognized by zinc finger domains. Asymmetric Z-sites were recombined by the concomitant action of two ZFRs with different zinc finger DNA-binding specificities, and could be recombined with a heterologous site in the presence of a third recombinase. Our results show that engineered ZFRs may be designed to promote site-specific recombination at many natural DNA sequence
Finger-tapping movements.
No full text(A) A tapping action with the left thumb and index finger is performed. (B) A tapping action with the right thumb and index finger is performed. (C) Tapping with the in-phase task is performed. (D) Tapping with the anti-phase task is performed. The distance between the thumb and index finger is kept between 3 and 4 cm, and movement was performed as fast as possible for 15 s.</p
Disruption of the developmental programme of Trypanosoma brucei by genetic ablation of TbZFP1, a differentiation-enriched CCCH protein
Full text linkThe regulation of differentiation is particularly important in microbial eukaryotes that inhabit multiple environments. The parasite Trypanosoma brucei is an extreme example of this, requiring exquisite gene regulation during transmission from mammals to the tsetse fly vector. Unusually, trypanosomes rely almost exclusively on post-transcriptional mechanisms for regulated gene expression. Hence, RNA binding proteins are potentially of great significance in controlling stage-regulated processes. We have previously identified TbZFP1 as a trypanosome molecule transiently enriched during differentiation to tsetse midgut procyclic forms. This small protein (101 amino acids) contains the unusual CCCH zinc finger, an RNA binding motif. Here, we show that genetic ablation of TbZFP1 compromises repositioning of the mitochondrial genome, a specific event in the strictly regulated differentiation programme. Despite this, other events that occur both before and after this remain intact. Significantly, this phenotype correlates with the TbZFP1 expression profile during differentiation. This is the first genetic disruption of a developmental regulator in T. brucei. It demonstrates that programmed events in parasite development can be uncoupled at the molecular level. It also further supports the importance of CCCH proteins in key aspects of trypanosome cell function
Finger-ring
No full textAN00755492_001_l. Silver wire spiral finger-ring. All these images are © Trustees of the British Museum. These free low-resolution images are covered also under the British Museum's Terms and Conditions (http://www.britishmuseum.org/about_this_site/terms_of_use/free_image_service.aspx). The full image collection is available at: http://www.britishmuseum.org/research/search_the_collection_database.aspx where you can also order high resolution copies.</p
A Shared History: Fayetteville and the University of Arkansas
No full textThree postcards on white card stock, each 5 1/4" wide x 3 1/4" highIllustrated by Helen Finger Leflar:The cards feature the Leflar home,Robert A. Leflar,Helen Finger Leflar, and their sons Charles J. Leflar and Robert B. Leflar
Finger-ring
No full textAN00755492_001_l. Silver wire spiral finger-ring. All these images are © Trustees of the British Museum. These free low-resolution images are covered also under the British Museum's Terms and Conditions (http://www.britishmuseum.org/about_this_site/terms_of_use/free_image_service.aspx). The full image collection is available at: http://www.britishmuseum.org/research/search_the_collection_database.aspx where you can also order high resolution copies.</p
A finger mechanism for adaptive end effectors
Full text linkThis paper presents design and analysis of a rigid link finger, which may be suitable for a number of adaptive end effectors. The design has evolved from an industrial need for a tele-operated system to be used in nuclear environments. The end effector is designed to assist repair work in nuclear reactors during retrieval operation, particularly for the purpose of grasping objects of various shape, size and mass. The work is based on the University of Southampton's Whole Arm Manipulator, which has a special design consideration for safety and flexibility. The paper discusses kinematic issues associated with the finger design, and to the end of the paper specifies the limits of finger operating parameters for implementing control law
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