121 research outputs found
STEM CELLS IN PEDIATRIC SARCOMAS.
No full textSarcomas represent a clinically and biologically diverse group of malignant connective tissue tumors. Despite aggressive conventional therapy, a large proportion of sarcoma patients experience disease recurrence which will ultimately result in mortality. The presence of a unique population of cells, referred to as cancer stem cells (CSC), have been proposed to be responsible for refractory responses to current chemotherapies as well underlying the basis for metastasis and relapse of disease - clinical corollaries to what has been termed the cancer stem cell hypothesis. The presence of CSCs have been suggested in a variety of hematologic and solid malignancies, and only more recently in sarcomas. Based on our current understanding of normal stem cell biology and evidence obtained from the study of malignant hematopoietic and solid tumors, researchers have identified candidate cell surface markers (CD133, CD117, Stro-1), biochemical markers (aldehyde dehydrogenase activity), and cytological characteristics (side population and spherical colony formation) that may identify putative sarcoma CSCs. In this review, we explore the current state of evidence that may suggest the existence of sarcoma CSCs. We present research in osteosarcoma, the Ewing’s sarcoma family of tumors, rhabdomyosarcoma, as well as other sarcoma subtypes to describe commonly used molecular and biochemical markers, as well as techniques, used in the identification, isolation and characterization of candidate sarcoma CSCs. We will also discuss the current controversies and challenges that face research in sarcoma CSC
El Tlacuache Núm. 34 (2002). 34 Año 2 (2002) marzo. El Tlacuache
No full text- Autenticidad dela imagen de la Virgen de Guadalupe en la tilma de Juan Diego por Laura Elena Hinojosa Hinojosa. - Nuestro patrimonio desconocido por Teresita Loera y Anaite Monterforte. - El Yauhtli por Margarita Avilés y Macrina Fuentes. - Trabajo de los metales por Alma Graciela de la Cruz S
Abstract 702: A novel cell-penetrating ATF5 antagonist peptide CP-d/n-ATF5 exerts <i>in vitro</i> and <i>in vivo</i> anti-tumor effects in a broad spectrum of pediatric cancers
No full textAbstract
Purpose: Activating transcription factor 5 (ATF5), a member of the ATF/CREB family transcription factor, has been implicated in the pathogenesis of glioblastoma and other adult tumors. Recently, a novel cell penetrating (CP-d/n-ATF5) peptide has been developed to antagonize ATF5 function. The goal of the current study is to test the efficacy of CP-d/n-ATF5 in several children tumors including neuroblastoma, hepatoblastoma, Ewing sarcoma, and rhabdoid tumor, in vitro and in vivo.
Methods: A panel of neuroblastoma cell lines: SK-N-Be(2)C, SK-N-DZ, NGP, IMR-32, NGP, SHEP-21N, KELLY, CHP-212, CHLA-20, CHLA-15 and SK-N-SH; hepatoblastoma cell lines HUH 6 and Hep-G2; Ewing sarcoma cell lines A673, SKNMC, SKNEP1, and TC32; and the rhabdoid cell G401, were treated with vehicle or 50, 100 and 200 μM of CP-d/n-ATF5. Cell viability and apoptosis were assessed after 72 hr by WST-8 and TUNEL assays, respectively. To test in vivo efficacy, SK-N-Be(2)C kidney xenograft tumors were treated with the peptide at dose 50mg/kg, IP injection once per day for first three days and then twice per week. Tumor growth was monitored by bioluminescence imaging and mice were sacked when flux reached a threshold value. Organ metastases were determined by ex vivo imaging. A patient-derived xenograft (PDX) model of rhabdoid tumors was employed where PDX tumors, at 150-200 mm3 size, were enrolled in the penetratin (control) and CP-d/n-ATF5 treatment (same dose as above). Tumors were measured biweekly with a calipers and mice were sacrificed when tumor volume reached a threshold (1500 mm3).
Results: CP-d/n-ATF5 exerted cytotoxicity or apoptosis, in a dose dependent manner, across a wide panel of pediatric tumor cell lines. In vitro, tumor cell viabilities were reduced 40-70% (P&lt;0.05) and apoptosis was increased 50-80% at 200 μM of CP-d/n-ATF5. In vivo, CP-d/n-ATF5 significantly inhibited SK-N-Be(2)C xenograft growth in nude mice, with a median survival of 35 days for control against 21 days for CP-d/n-ATF5, P=0.0013. CP-d/n-ATF5 also reduced SK-N-Be(2)C metastatic burden in the liver (P&lt;0.05) and bone marrow (P&lt;0.01). In the rhabdoid PDX model, there was a significant inhibition of tumors treated with CP-d/n-ATF5 as compared to Penetratin treatment, with a mean tumor volume of control 1283 ± 266.6 mm3 (n=5) vs CP-d/n-ATF5 234.2 ± 50.66 mm3 (n=6), at day 10 post treatment. Penetratin treated tumors showed a median post-treatment time of 13 days to reach threshold volume. None of the CP-d/n-ATF5 treated tumors reached the threshold after 28 days of treatment with some tumors demonstrating regression, indicating a profound anti-tumor effect of the peptide.
Conclusion: Our study shows that a novel ATF5-targeting peptide CP-d/n-ATF5 has broad and profound cytotoxic and apoptotic effects in several pediatric tumors in vitro and in vivo. Our study also indicates that CP-d/n-ATF5 has the potential to act as an anti-metastatic agent.
Citation Format: Debarshi Banerjee, Shuobo Boboila, Cherease Street, Shunpei Okochi, Filemon S. Dela Cruz, Eileen Connolly, Angela Kadenhe-Chiweshe, Darrell Yamashiro. A novel cell-penetrating ATF5 antagonist peptide CP-d/n-ATF5 exerts in vitro and in vivo anti-tumor effects in a broad spectrum of pediatric cancers [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 702. doi:10.1158/1538-7445.AM2017-702</jats:p
Ranilo Balaguer Hermida Imagining Modern Democracy: A Habermasian Assessment of the Philippine Experiment
No full textPusong Mamon: Nel Noddings\u27s Care Ethics in the Filipino Educational Setting
No full textThis paper evaluates Nel Noddings’s view of moral education in the context of teaching in a Philippine university. It first presents the key aspects of Noddings’s ethical theory, identifying her three strategic moves as follows: the dissolution of the public/private split, the replacement of justice-based punishment system with a caring system, and the promotion of care work as the core of education itself. Through a discussion of two cases of academic dishonesty, the author assesses the merits of these strategies and recommends a re-conceptualization both of care ethics and the existing educational paradigm
Recovery Strategy of Selected Filipino Restaurants in Tagaytay City
No full textAbstract: There have been a lot of people visiting Tagaytay City as one of the best tourist spots in Cavite. Due to the natural disaster – The Taal Volcano eruption, many establishments have been greatly affected especially Filipino Restaurants with alfresco dining and those near the Taal volcano. Because of the disaster, the researchers aimed to determine and analysed the gathered data concerning the significant difference of recovery strategy of selected Filipino restaurants with alfresco dining in Tagaytay City. The study's main concept is a recovery strategy. This concept has 5 factors examined by the researchers including Human Resources, Natural Resources, Capital Resources, Technology, Social and Political. Descriptive methods and online survey questionnaires via google forms were used to collect data and information. The respondents were the selected restaurant’s Managers and staff. The results show that the restaurants recovered by focusing on Human resource, Technology, Social and Political factors. The respondents perceived that giving proper training and seminars greatly benefited the recovery of the establishment. The results also show that the selected restaurants have no significant difference between demographic profile, and factors such as Human resources, Natural resources, Capital resources, technology, and Social and Political.
Keywords: Natural Disaster, Quantitative Research, Recovery Strategy, Tagaytay City.
Title: Recovery Strategy of Selected Filipino Restaurants in Tagaytay City
Author: Lorenzo Martin Becina, Margoe Dela Cruz, Darren Angelo Torres, Ailene Rolle
International Journal of Social Science and Humanities Research
ISSN 2348-3156 (Print), ISSN 2348-3164 (online)
Vol. 10, Issue 3, July 2022 - September 2022
Page No: 32-49
Research Publish Journals
Website: www.researchpublish.com
Published Date: 14-July-2022
DOI: https://doi.org/10.5281/zenodo.6831127
Paper Download Link (Source)
https://www.researchpublish.com/papers/recovery-strategy-of-selected-filipino-restaurants-in-tagaytay-cityInternational Journal of Social Science and Humanities Research, ISSN 2348-3156 (Print), ISSN 2348-3164 (online), Research Publish Journals (Publisher), Website: www.researchpublish.co
Best Practices in Managing Customers Complaints in Selected Quick Service Restaurants in Dasmariñas City
No full textAbstract: This research was carried out to find out the best practices for dealing with customer complaints in Dasmarinas City's quick-service restaurants. The researchers of this study used the Purposive Sampling Technique to perform a descriptive survey among twenty QSR customers and ten QSR managers to learn about their insights founded on their own customer complaint experiences. Through data gathering, this study was able to find out that the demographic profile of customers affects the way they address their concerns to a QSR manager. Conversely, the demographic profile of the QSR managers affects the way they address the concerns of their customers. Most especially, their experiences from their educational attainments, given that the majority reached the college level, up to their working attainments where the majority are employees, make up the behaviors they have in a QSR. This study obtained information such as recommendations from all respondents about the best practices in managing customer complaints in QSRs, which whoever needs to know, may utilize for their own good such as students, researchers, and businesses.
Keywords: customer complaint management, customer satisfaction, food industry, quick service restaurant, QSR.
Title: Best Practices in Managing Customers Complaints in Selected Quick Service Restaurants in Dasmariñas City
Author: Maricris R. Ayate, Jhen Dela Cruz, Laney Valerie V. Buquid, Mae Casiles
International Journal of Thesis Projects and Dissertations (IJTPD)
Vol. 10, Issue 2, April 2022 - June 2022
Page No: 48-57
Research Publish Journals
Website: www.researchpublish.com
Published date: 28-June-2022
DOI: https://doi.org/10.5281/zenodo.6772549
Paper Download Link (Source)
https://www.researchpublish.com/papers/best-practices-in-managing-customers-complaints-in-selected-quick-service-restaurants-in-dasmarias-cityInternational Journal of Thesis Projects and Dissertations (IJTPD), Research Publish Journals (Publisher), Website: www.researchpublish.co
Pusong mamon: Nel Nodding\u27s care ethics in the Filipino educational setting
No full textThis paper evaluates Nel Noddings’s view of moral education in the context of teaching in a Philippine university. It first presents the key aspects of Noddings’s ethical theory, identifying her three strategic moves as follows: the dissolution of the public/private split, the replacement of justice-based punishment system with a caring system, and the promotion of care work as the core of education itself. Through a discussion of two cases of academic dishonesty, the author assesses the merits of these strategies and recommends a re-conceptualization both of care ethics and the existing educational paradigm
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