21 research outputs found
Prevalence of pathogenicity groups of<i>Leptosphaeria maculans</i>in western Canada and North Dakota, USA
No full textComparative screening of bacteria for biological control of potato late blight (strain US-8), using invitro, detached-leaves, and whole-plant testing systems
No full textSeasonal and daily variation in the airborne concentration of<i>Gibberella zeae</i>(Schw.) Petch spores in Manitoba
No full textTemporal and spatial dynamics of mycosphaerella blight [<i>Mycosphaerella pinodes</i>] in field pea
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A single amino acid mutation in Spo0A results in sporulation deficiency of Paenibacillus polymyxa SC2
No full textAbstractSporulating bacteria such as Bacillus subtilis and Paenibacillus polymyxa exhibit sporulation deficiencies during their lifetime in a laboratory environment. In this study, spontaneous mutants SC2-M1 and SC2-M2, of P. polymyxa SC2 lost the ability to form endospores. A global genetic and transcriptomic analysis of wild-type SC2 and spontaneous mutants was carried out. Genome resequencing analysis revealed 14 variants in the genome of SC2-M1, including three insertions and deletions (indels), 10 single nucleotide variations (SNVs) and one intrachromosomal translocation (ITX). There were nine variants in the genome of SC2-M2, including two indels and seven SNVs. Transcriptomic analysis revealed that 266 and 272 genes showed significant differences in expression in SC2-M1 and SC2-M2, respectively, compared with the wild-type SC2. Besides sporulation-related genes, genes related to exopolysaccharide biosynthesis (eps), antibiotic (fusaricidin) synthesis, motility (flgB) and other functions were also affected in these mutants. In SC2-M2, reversion of spo0A resulted in the complete recovery of sporulation. This is the first global analysis of mutations related to sporulation deficiency in P. polymyxa. Our results demonstrate that a SNV within spo0A caused the sporulation deficiency of SC2-M2 and provide strong evidence that an arginine residue at position 211 is essential for the function of Spo0A
Leaf-wetness duration and temperature required for infection of saskatoon leaves by<i>Entomosporium mespili</i>under controlled conditions
No full textThe development of a dynamic diseaseforecasting model to control<i>Entomosporium mespili</i>on<i>Amelanchier alnifolia</i>
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