1,721,244 research outputs found

    The <i>R. felis</i> plasmids.

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    <p>Specific PCR amplification of the two <i>R. felis</i> plasmid forms. Lane 1: Molecular size (bp); lane 2: pRFa-pRFb amplicon; lane 3: pRFc-pRFd amplicon; lane 4: pRFa-pRFd amplicon; b) Schematic representation of <i>R. felis</i> plasmids indicating the position of PCR primers used to amplify the pRF (pRFa/pRFb and pRFc/pRFd primer pairs) and pRFδ (pRFa/pRFd primer pair) plasmids.</p

    Genome Comparisons of <i>R. felis</i> and <i>R. conorii</i>

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    <div><p>Red dots represent homologous genomic segments greater than 150 bp identified by BLASTN (<i>E</i>-value < 10<sup>−3</sup>).</p> <p>(A) Comparison between <i>R. felis</i> and <i>R. conorii</i> chromosomes. Vertical and horizontal green lines indicate the positions of transposase ORFs in <i>R. felis</i> and in <i>R. conorii</i>, respectively.</p> <p>(B) Self-comparison of <i>R. felis</i> chromosome.</p></div

    Visualization of <i>R. felis</i> Pili by Transmission Electron Microscopy

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    <div><p>Bacteria collected from the supernatant of <i>R. felis</i>–infected XTC cells were negatively stained.</p> <p>(A) Sexual pilus observed between two bacteria.</p> <p>(B) <i>R. felis</i> also possesses small appendages likely to be fimbriae pili.</p></div

    Sequencing results for <i>Rickettsia</i> spp. positive <i>C. felis</i> collected from dogs.

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    <p>For each gene amplified and sequenced (<i>17 kDa</i>, <i>ompB</i> and <i>ompA</i>), the Accession Number (A.N.) of the corresponding sequence submitted in GenBank and the percentage (%) of identity with respect to reference sequence of <i>R. felis</i> present in GenBank are reported.</p><p>Sequencing results for <i>Rickettsia</i> spp. positive <i>C. felis</i> collected from dogs.</p

    Sequencing results for <i>Rickettsia</i> positive fleas <i>C. felis</i> collected from cats.

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    <p>For each gene amplified and sequenced (<i>17 kDa</i>, <i>ompB</i> and <i>ompA</i>), the Accession Number (A.N.) of the corresponding sequence submitted in GenBank and the percentage (%) of identity with respect to reference sequence of <i>R. felis</i> present in GenBank are reported.</p><p>Sequencing results for <i>Rickettsia</i> positive fleas <i>C. felis</i> collected from cats.</p

    Treatment of <i>H. felis</i> infected mice with long term IL-12 therapy is protective.

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    <p>Balb/c mice were infected with <i>H. felis</i> and treated with IL-12 intermittently for 12 weeks. Control mice were either infected with <i>H. felis</i> without treatment, or treated with IL-12 in the absence of infection. Mice were harvested at 12 weeks and longitudinal biopsies encompassing the entire length of the gastric mucosa were prepared for histologic analysis. (a) H&E stained sections were used to independently grade the antrum and fundus for inflammation using scales of 0–3 and 0–10 respectively. (b) Silver stained sections were used to visualize <i>H. felis</i> organisms and bacterial load was determined by counting the number of infected glands per linear millimeter in the antrum and fundus. n = 5–8 mice/group.</p
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