323,334 research outputs found

    Despues de Dios la primera obligacion, y glosa de ordenes militares

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    ÍndicesSign.: a-d\p4\s, A-Z\p4\s, 2A-2Z\p4\s, 3A-3R\p4\

    Glycoconjugate expression changes during Rana dalmatina early development

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    Glycoconjugates are generally involved in cell adhesion and morphogenetic movements. To investigate their distribution and expression changes during amphibian development, several FITC-or HRP-labeled lectins were used in Rana dalmatina embryos and larvae. During gastrulation, WGA, ConA, HPA, GSA-I, PNA and RCA-I labeled either the envelopes on the perivitelline space-contained substances, while ectodermal cells and/or extracellular matrix were mainly labeled by WGA, GSA-I, DBA and LTA. In these structures fibronectin-like molecules were also found. ConA, HPA, RCA-I, SBA also labeled ectoderm, but with lower affinity. At larval stages, ectodermal derivatives, such as larval skin, central nervous system, eye and ear, were also strongly labeled by WGA, DBA and LTA, while the affinity to ConA, HPA, GSA-I, SBA, appeared less visible; the affinity sites to RCA-I and PNA were totally lacking. Fibronectin-like immunoreactivity was hardly present at the larval stages. These results point out that glycoconjugate expression changes in cell surface and in extracellular matrix might be developmentally regulated. For this reason the different glycoconjugates seem to play stage-specific roles in early development

    Developmental regulation of lectin-binding patterns in Paracentrotus lividus gonads, gametes, and early embryos

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    By use of several lectins (ConA, WGA, SBA, GS I, PNA), a study was carried on gametes and developing embryos of the sea urchin Paracentrotus lividus, to investigate developmental changes in cell surface, leading to changements in cell-environment interactions

    Interaction of monensin on dimorphic transition and hyphal growth in Candida albicans

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    Monensin, an exocytosis perturber, was assayed on the dimorphic fungus Candida albicans in order to study its effects on wall synthesis and morphogenesis. In the presence of the drug, both germ-tube formation and hyphal elongation were markedly inhibited according to the dose and exposure time. The effect was accompanied by morphogenetic malformations which could be detected under UV light and by transmission electron microscopy. Enormous wall and septa thickenings revealed by cytochemical tests indicated an abnormal deposition of chitin. Since chitin synthase is inserted in the plasma membrane in a latent state and its activation depends on regulatory factors reaching the cell periphery through an orderly exocytosis, it is assumed that monensin affects the vesicular traffic leading to a prevalence of activators over inhibitors of the enzyme
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