124 research outputs found
When We Kneel, We Learn
No full textWhen We Kneel, We Learn is an autobiographical work based on Kayla Dudley’s experience in a caregiving facility over the expanse of two years. The book touches on the relationships she cultivated with the elderly -- including those living with Alzheimer’s and dementia. The audience that the author hopes to reach are those that are interested in caring for the geriatric population, through the lens of Christianity. However, the lessons that are learned throughout the piece are so profoundly human, that they are accessible to anyone who wants to deepen their relationships and have a greater understanding of others. The memoir is shaped in such a way that it gives the reader insight into the personalities of our elders, including quotes and stories from individual persons, as well as ways that Kayla learned to approach caring for those enduring the effects of aging.Englis
2016-08-25 Fast Walstrom Outlaw Supplemental File.docx
No full text2016-08-25 Fast Walstrom Outlaw Supplemental File.doc
Fertilization unconventionally activates phospholipase C during the fast block to polyspermy in the African clawed frog, Xenopus laevis
No full textFor most animals, fertilization by more than one sperm is lethal to a developing embryo. Accordingly, eggs use multiple mechanisms to keep additional sperm from entering the nascent zygote. One such mechanism, the fast block to polyspermy, is used by externally fertilizing animals and involves a change in the membrane potential of the egg from a negative resting potential to a positive fertilization potential. Sperm can bind to, but not enter, depolarized eggs. In frogs, fertilization opens the calcium-activated chloride channel TMEM16A, allowing an efflux of chloride ions that depolarize the egg’s membrane. Currently, the signaling pathway by which fertilization opens TMEM16A is not yet known.
Using whole-cell recordings on Xenopus laevis eggs during fertilization, I have found that inhibiting phospholipase C (PLC) using the general PLC inhibitor U73122 abolishes the fast block depolarization, and polyspermic fertilization occurred. U73122 covalently modifies PLC, which enabled me to independently treat either egg or sperm prior to fertilization to identify which gamete provides the PLC. Whole-cell fertilization recordings with U73122 pretreated sperm showed typical fast block depolarizations and monospermic development, while pretreated eggs did not exhibit a depolarization and exhibited polyspermic development, indicating that the PLC necessary for the fast block is egg-derived. X. laevis eggs have three PLC subtypes (PLCg1, PLCb1, and PLCb3). Each PLC subtype typically uses different activation methods: PLCg1 is canonically activated by tyrosine phosphorylation, and PLCb is typically activated through the Gaq/11 subunit of G-protein coupled pathways. Inhibiting these methods of canonical activation revealed typical depolarizations during whole-cell recordings and monospermic development. Together, these data reveal that the egg PLC during the fast block to polyspermy in X. laevis is activated by a novel signaling mechanis
Pushing for Support of Medical Marijuana
No full textThe negative stigma associated with marijuana use and its users prevents doctors from being able to make objective decisions about whether or not marijuana use is appropriate for their patient.Fall 201
Dog Birth Certificate
No full textThe dog birth certificate legislation will enforce every dog’s PIN, license and microchip number with a state agency for the safety of the pet and owner.Fall 201
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Ideographs, Fragments, and Strategic Absences: An Ideographic Analysis of <Collateral Damage>
No full textThis study examined the ideograph of through an analysis of the Bush Administration's rhetoric as well as visual photographs of Iraqi civilian deaths. The project argues that the psycho-dynamic rhetoric of the Bush Administration during a time of visual censorship lead to the dehumanization of Iraqi civilian deaths during the War in Iraq. The method consisted of a textual analysis of the Bush Administration's rhetoric and continued with a content analysis of news media's photographs. The author argues that critics gain a deeper understanding of the disappearing dead phenomenon of Iraqi civilians by examining ideographic fragments of psycho-dynamic rhetoric
Do men deserve more credit? A study on gender and caregiving
No full textCare for the elderly population in the United States is fast becoming an issue many families are facing. This study investigated the relationship between gender and willingness of college students to care for aging parents by surveying 24 male and female undergraduate and graduate students at the University of Wisconsin-Stout. It was hypothesized that female college students would be more willing than men to care for aging parents in the assistance categories of personal hygiene and housework. Survey data was statistically analyzed using cross-tabulations, and mean comparisons. Results indicated that males are more willing to provide care in the categories of personal hygiene and housework. These findings did not support the hypothesis of females being more willing to provide care; the implications are that the traditional roles and stereotypes need to be reassessed
Goal Setting and Student Achievement: Effects of Student Goals and Achievement on Fluency Probes for Special Education Students
No full textThe purpose of this study was to investigate the effects of goal setting on student achievement in 3rd and 4th grade special education students on reading assessments. The participants in this study were three 3rd grade and four 4th grade special education students between the ages of 9 and 10 years of age. Three of the participants were female and four were male, four students were African American, one was Caucasian and two were Hispanic. This study took place in an elementary school in a suburban school district in the Midwest. The eight week, mixed methods action research study examined the relationship between goal setting with 3rd and 4th grade special education students and their reading fluency utilizing the Formative Assessment System for Teachers (FAST) which is used by the school district. A pre- and post-survey regarding the students' attitudes toward reading and their self-efficacy in regards to reading was used. Students also were asked a question after their goal setting, but before they started their probe. They all did the pre- and post- FAST fluency test. The results of this research indicated that the students' words per minute (WPM) increased over the eight weeks of the study. The open-ended, self-efficacy questionnaire indicated that most students think highly of themselves when it comes to reading. Future studies investigating goal setting with special education readers would be beneficial because of the motivation and positive self-efficacy my students demonstrated
Designing a Single Protein‐Chain Reporter for Opioid Detection at Cellular Resolution
No full textMu‐opioid receptor (MOR) signaling regulates multiple neuronal pathways, including those involved in pain, reward, and respiration. To advance the understanding of MOR’s roles in pain modulation, there is a need for high‐throughput screening methods of opioids in vitro and high‐resolution mapping of opioids in the brain. To fill this need, we designed and characterized a genetically encoded fluorescent reporter, called Single‐chain Protein‐based Opioid Transmission Indicator Tool for MOR (M‐SPOTIT). M‐SPOTIT represents a new and unique mechanism for fluorescent reporter design and can detect MOR activation, leaving a persistent green fluorescence mark for image analysis. M‐SPOTIT showed an opioid‐dependent signal to noise ratio (S/N) up to 12.5 and was able to detect as fast as a 30‐second opioid exposure in HEK293T cell culture. Additionally, it showed an opioid‐dependent S/N up to 4.6 in neuronal culture and detected fentanyl with an EC50 of 15 nM. M‐SPOTIT will potentially be useful for high‐throughput detection of opioids in cell cultures and cellular‐resolution detection of opioids in vivo. M‐SPOTIT’s novel mechanism can be used as a platform to design other G‐protein‐coupled receptor‐based sensors.A genetically‐encoded fluorescent sensor was designed for the mu‐opioid receptor using a novel sensor mechanism. In the absence of opioids, Nb39 interacts with the circular permuted green fluorescent protein (cpGFP) and inhibits the fluorophore maturation of cpGFP. When the opioid binds to the opioid receptor (OR), Nb39 binds to the intracellular side of the activated OR. This removes Nb39 from cpGFP, allowing the cpGFP fluorophore to mature, resulting in an opioid‐induced fluorescence increase.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/167815/1/anie202101262_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/167815/2/anie202101262-sup-0001-misc_information.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/167815/3/anie202101262.pd
The complete mitochondrial genome of the Tennessee Dace (Chrosomus tennesseensis)
No full textThe Tennessee Dace, Chrosomus tennesseensis (Starnes and Jenkins 1988), is a small minnow (Cypriniformes: Leuciscidae) found in the upper Tennessee River watershed and Graves Creek, in the Mobile River watershed. Chrosomus tennesseensis occurs sporadically throughout its range and has been listed as vulnerable by the IUCN (NatureServe). Until recently, C. tennesseensis had been known only to occur in the upper Tennessee River watershed, however, it has been discovered in headwaters of the Black Warrior River of the Mobile River watershed. We sequenced the mitochondrial genome of C. tennesseensis collected in the Mobile River watershed to better understand the colonization of the Mobile River watershed and the interspecific relationships of Chrosomus. Furthermore, the availability of the mitochondrial genome will assist in designing specific environmental DNA (eDNA) primers that will allow for less intrusive sampling of threatened and endangered Chrosomus species
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