1,721,038 research outputs found
Post translational control of the PML/RARalpha protein and retinoic acid resistance in Acute Promyelocytic Leukemia (APL)
No full textDerivative of [(3-hydroxy-4-pyron-2-yl)-methyl]-amine and use thereof as anti-neoplastic drugs
No full textThe present invention relates to compounds heaving general formula (I), i.e. poly-alkyl-bis-maltolic molecules and in particular to derivates of [(3-hydroxy-4-pyron-2-yl)methyl]-amine and pharmaceutically accetable salts thereof, and to the use thereof as anti-neoplastic drugs, in particular, for a preparation of a medicament for the treatment of neoplastic disease
DERIVATI DI [(3-IDROSSI-4-PIRON-2-IL)METIL]-AMINA E LORO USO COME FARMACI ANTI-NEOPLASTICI
No full textPharmaceutical composition of [(3-hydroxy-4 -pyron-2-yl) methyl] -amine derivatives and DNA demethylating agents and their use as anti-neoplastic drugs
No full textThe present invention concerns pharmaceutical compositions comprising compounds with general formula I or polyalkyl-bis- maltolic molecules and in particular derivatives of [(3-hydroxy-4- pyron-2-yl)methyl]-amine in combination with DNA demethylating agents as anti-neoplastic drugs. In particular, for the preparation of a medicament for the treatment of neoplastic pathologies
DERIVATIVES OF [(3-HYDROXY-4-PYRON-2-YL)-METHYL]-AMINE AND USE THEREOF AS ANTI-NEOPLASTIC DRUGS
No full text
The Current State of Chromatin Immunoprecipitation (ChIP) from FFPE Tissues
Full text link: Cancer cells accumulate epigenomic aberrations that contribute to cancer initiation and progression by altering both the genomic stability and the expression of genes. The awareness of such alterations could improve our understanding of cancer dynamics and the identification of new therapeutic strategies and biomarkers to refine tumor classification and treatment. Formalin fixation and paraffin embedding (FFPE) is the gold standard to preserve both tissue integrity and organization, and, in the last decades, a huge number of biological samples have been archived all over the world following this procedure. Recently, new chromatin immunoprecipitation (ChIP) techniques have been developed to allow the analysis of histone post-translational modifications (PTMs) and transcription factor (TF) distribution in FFPE tissues. The application of ChIP to genome-wide chromatin studies using real archival samples represents an unprecedented opportunity to conduct retrospective clinical studies thanks to the possibility of accessing large cohorts of samples and their associated diagnostic records. However, although recent attempts to standardize have been made, fixation and storage conditions of clinical specimens are still extremely variable and can affect the success of chromatin studies. The procedures introduced in the last few years dealt with this problem proponing successful strategies to obtain high-resolution ChIP profiles from FFPE archival samples. In this review, we compare the different FFPE-ChIP techniques, highlighting their strengths, limitations, common features, and peculiarities, as well as pitfalls and caveats related to ChIP studies in FFPE samples, in order to facilitate their application
Premature senescence induced by DNA demethylating agent (Decitabine) as therapeutic option for malignant pleural mesothelioma.
Full text linkThe drug-dependent induction of premature senescence in neoplastic cells is considered per se an important tumor suppressive mechanism. DNA demethylating agents recently introduced in clinical trials, such as 5-aza-cytidine (Decitabine) and its derivatives, have been extensively characterized in recent years as antiproliferative compounds that act through multiple mechanisms, which have not yet been fully clarified. We recently analyzed the introduction of Decitabine in therapy for malignant pleural mesothelioma (MPM) observing that, despite the ability to induce profound biological effects in MPM cells, the drug failed to generate a massive apoptotic response. Since one of the most intriguing aspects of DNA demethylating agents is the possibility to accelerate the senescent response of tumor cells, we investigated the hypothesis of Decitabine inducing, in vitro, the premature aging of MPM cells
Constitutive degradation of the PML/RARalpha protein is present in retinoic acid (RA)-resistant acute promyelocytic leukemia cells and involves the proteasome proteolytic pathway
No full textThe dark side of histones: genomic organization and role of oncohistones in cancer
Full text linkThe oncogenic role of histone mutations is one of the most relevant discovery in cancer epigenetics. Recurrent mutations targeting histone genes have been described in pediatric brain tumors, chondroblastoma, giant cell tumor of bone and other tumor types. The demonstration that mutant histones can be oncogenic and drive the tumorigenesis in pediatric tumors, led to the coining of the term "oncohistones." The first identified histone mutations were localized at or near residues normally targeted by post-translational modifications (PTMs) in the histone N-terminal tails and suggested a possible interference with histone PTMs regulation and reading
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