1,721,051 research outputs found
PLASMID FUNCTIONS INVOLVED IN THE STABLE PROPAGATION OF THE PKD1 CIRCULAR PLASMID IN KLUYVEROMYCES-LACTIS
No full textPlasmid factors involved in the stable propagation
of pKD1-derived vectors in Kluyveromyces lactis
transformants have been identified. Three genes (A, B
and C) have been found to be present in pKD1: the interruption of the B and C genes led to high plasmid instability. Stability could be restored in trans when host cells
contained pKD1 as the resident plasmid (pKD1+
strains). The A gene, which codes for a site-specific recombinase, did not affect plasmid partitioning. Vectors
bearing only the pKD1 replication origin (or a chromosomal
ARS), and no other pKD1 sequence, were very
unstable both in the presence and absence of the resident
plasmid in host cells. These vectors could be stabilized in
pKD1+ strains, but not in pKDI- strains, by the insertion
of a 200 bp-long pKDI sequence. This sequence,
called the cis-acting stability locus (CSL), together with
the products of the B and C genes, ensured plasmid partitioning at cell divison. Possible hairpin structures and
direct repeats were regularly spaced within the CSL. This
region, and the corresponding cis-acting stabilizing elements
of other yeast plasmids, did not have sequence
homology but shared some structural regularities
Analysis of the plasmid population in Kluyveromyces lactis transformants: recombination, stability and copy number
No full textPlasmid elements involved in the stale propagation of pKD1-derived vectors in Kluyveromyces lactis transformants
No full textAnalisi di mutazioni nel gene mitocondriale per il tRNAleu di S.cerevisiae correlate alle patologie umane
No full textActive recombination of pKD1 derived vectors with resident pKD1 in Kluyveromyces lactis transformation
Full text linkThe host specificity of the 2 u-like circular
plasmid pKD1 is such that this plasmid replicates
stably in several species of Kluyveromyces yeasts, but
not in Saccharomyces cerevisiae, pKD1-derived plasmids
containing various parts of the pKD1 sequence
were capable of transforming Kluyveromyces lactis
with high efficiency. When such vectors were introduced
into host strains that contained resident pKD1
plasmid, the input DNA frequently recombined with it
to produce high proportions of additive recombinant
molecules that replicate stably. Recombination events
were shown to occur with vectors differing for the
presence or absence of the putative origin of replication
and of the inverted repeats. Structure, stability and
copy number of the recombination products were
analyzed for various types of vectors
Isolation and sequence analysis of a Kluyveromyces lactis chromosomal DNA element able to autonomously replicate in S. cerevisiae and K. lactis
No full textVectors for the cloning and expression of heterologous genes in yeast and the yeast strains transformed by said vectors.
No full textIdentification of an essential core element and stimulatory sequences in a Kluyveromyces lactis ARS element KARS101.
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