1,721,002 research outputs found
Erratum: Engraftment, neuroglial transdifferentiation and behavioral recovery after complete spinal cord transection in rats (Surg Neurol Int 2018 9 :19 DOI: 10.4103/sni.sni_369_17)
No full textIn the article titled “Engraftment, neuroglial transdifferentiation and behavioral recovery after complete spinal cord transection in rats”, published on pages 19, Issue 1, Volume 9 of Surgical Neurology International,[1] the name of the authors is written incorrectly as The “How to cite this article” section should read correctly as “Luzzi S, Crovace AM, Lacitignola L, Valentini V, Francioso E, Rossi G, Invernici G, Galzio RJ, Crovace A”
Trattamento delle lesioni tendinee e legamentose nel cavallo mediante trapianto autologo di cellule mononucleate di midollo osseo (BMMNCs)
No full textRigenerazione tendinea in seguito a trapianto autologo di cellule mononucleate di midollo osseo in lesioni sperimentali del tendine flessore superficiale delle falangi nel cavallo
No full textUSE OF AUTOLOGOUS BONE MARROW STROMAL CELLS (BMSC) AND RESORBABLE BONE GRAFT SUBSTITUTE IN DOGS WITH BONE-LOSS
No full textHistology and immunohistochemistry study of ovine Achille’s tendon grafted with Cultured Bone Marrow Mesenchymal Cells and Bone Marrow Mononucleated Cells after collagenase-induced tendonitis
No full textThe aim of this study was to compare the regeneration abilities of cultured bone marrow mesenchymal cells (cBMSC) and bone marrow mononuclear cells (BMMNC) with fibrin glue, saline solution and sham control in collagenase-induced tendinitis of the Achilles tendon in sheep.
METHODS:
Six sheep were recruited randomly to each group: cBMSC, BMMNC, fibrin, saline and sham control. Each group received the relative treatment two weeks after inducing lesions (T(0)). After eight weeks (T(8)) of treatment, the tendons were harvested and evaluated for histomorphology, Collagen type I, III, Cartilage Oligomeric Matrix Protein (COMP) and CD34 positive cells expression.
RESULTS:
Histology and immunohistochemistry showed similar capabilities of cBMSC and BMMNC to restore the architecture of fibres and Extra Cellular Matrix (ECM), with a high expression of collagen type I and COMP and a very low expression of collagen type III in treated tendons. The complete architectural disruption of fibres, dramatic reduction of collagen Type I and COMP expression and increase collagen type III expression were commonly observed in tendons treated with fibrin or saline only. The presence of CD34 positive cells was appreciable in the BMMNC group while few cBMSC showed this cluster of differentiation, not expressed in tendons treated with fibrin or saline.
CLINICAL SIGNIFICANCE:
The data in this study show the efficacy of cBMSC and BMMNC in regenerating tendon tissue after collagenase-induced tendinitis
Survival of mesenchymal stem cells in collagenase induced tendonitis in an ovine model.
Full text linkThe pourpose of this study was to evaluate the efficacy and
the survival of local injection of allogenic MSC marked with
Red Fluorescent Protein (RFP) (Lentigen-Italy) in
collagenase induced tendonitis in the ovine Achille's
tendon.The study was performed after the approval by the
National Animal Care and Use Committee
Four sheep (2 years old, female, 45 bwt) have been
enrolled in the study. After some days for the acclimatation,
the sheep have been investigated to exclude any previous
Achilles' tendon lesion. Three weeks before starting of the
study one sheep was randomly selected for Bone Marrow
harvesting for MSCs cultivation. The MSCs obtained has
been trasfected with a lentivirus for integration of a gene for
expression of Red Fluorescent Protein (RFP). After a week
the other 3 sheep was injected in both Achilles' tendon with
400 U.I. of Collagenase IA of Cl. hystoliticum (Sigma-
Aldrich-Italy). After two weeks the left Achilles' tendon of
each sheep was injected with a solution of 6x106 RFPMSCs
( MSCRFP ) in 1 ml of fibrine glue (TISSUCOL ,
Baxter). The remaining tendons were used as negative
control and received the same volume of saline solution as
placebo. At 3-4-6 weeks from the treatment the tendons
were harvested and evaluated for morphology, collagen I
and III expression, and visualized at fluorescence
microscope to assess RFP expression of the grafted
MSCRFP. The results of these investigations evidenced
the presence of MSCRFP in the treated
tendons respect to the control ones at 3, 4 and 6 weeks after
the treatment. Moreover, the RFP positive tissue showed high
expression of collagen I and low collagen III with good
morfphology in comparison to the lesions treated with
placebo.The presence of high expression of collagen I and
low collagen III with good morfopholgy, in term of restored
tendon architecture, can be related to the MSCRFP injected
into tendon lesions, as a large number of cells can survive in
the site of injection.
These results showed that intralocal administration of MSCs
into the tendon lesion can lead to a good effect on injured
tendon . The local infusion delivery entails injecting MSCs
directly into the tissue of interest and guarantees a higher
number of engrafted cells and optimal therapuetic effect.
Besides the survival of high numbers of positive RFP -MSCs
in treated samples have been demonstrated at 3, 4 (Fig.1)
and 6 weeks from the treatment. We have evaluated also that
quality of tendon healing in MSCRFP treated tendons has
been based on a better architecture of collagen fibers and
high expression of collagen I respect to collagen III (Fig.2),
related to the control tendons.
The data obtained in this study confirm that MSCs allograft
have a positive effect on tendon healing, its lack of significant
immunogenicity permitting allogenic transplantation without
immunosuppressive drugs and that the local injection in the
tendon allows the survival of MSCs with good engraftment
effincency
Histology and immunohistochemistry study of ovine tendon grafted with cBMSCs and BMMNCs after collagenase-induced tendinitis.
No full textSurvival of bone marrow mesenchymal stem cells labelled with red fluorescent protein in an ovine model of collagenase-induced tendinitis
Full text linkObjective: The aim of this study was to track the survival and efficacy of allogeneic bone marrow mesenchymal stem cells (BM-MSC) marked with red fluorescent protein (BM-MSCRFP) in an ovine model of collagenase-induced tendinopathy. Methods: Bone marrow was harvested from one donor sheep and BM-MSC were isolated, cultivated and transfected with red fluorescent protein (BM-MSCRFP). Collagenase was injected into both Achilles tendons in the remaining nine sheep. After two weeks the left tendon was injected with a solution of 6 x 106 BM-MSCRFP and fibrin glue, while only fibrin glue was administered to the contra-lateral tendon in each sheep. After three, four and six weeks the tendons were harvested and evaluated for morphology, collagen I deposition, presence of CD34+ cells, and fluorescent labelled BM-MSC. Results: We demonstrated that delivery of BM-MSC into tendon lesions had positive effects on the injured tendons. The BM-MSCRFP survived at three, four and six weeks after treatment, leading to better quality healing of tendons as compared to the controls, where no labelled cells were detected. Interestingly, we demonstrated high expression of CD34+ cells in tendons that had been treated with BM-MSCRFP. Clinical relevance: Mesenchymal stem cell allografts have a positive effect on tendon healing and local injection of BM-MSC directly into the tendon allows the homing of BM-MSC for good efficiency of engraftment
Cultured bone marrow mesenchymal stem cells and bone marrow mononucleated cells in tendon injury repair : experiences in sheep’s and horses’s experimental tendinitis model
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