63,466 research outputs found
Il gel piastrinico nel trattamento del “piede diabetico”: esperienza preliminare
No full textIL GEL PIASTRINICO NEL TRATTAMENTO DEL “PIEDE DIABETICO”: ESPERIENZA PRELIMINARE
Massimo Chiaretti*, Giancarlo Ferrazza+, Rita Maria Fracassi@ , Andrea Negro°, Domenico Tuscano*
(*) Dipartimento di Chirurgia Generale, Specialità Chirurgiche e Trapianti d’Organo “Paride Stefanini” Azienda Policlinico Umberto I, Università degli Studi di Roma “La Sapienza”
(+ ) Servizio di Immunoematologia e Medicina Trasfusionale Azienda Policlinico Umberto I, Università degli Studi di Roma “La Sapienza”
(@) Servizio di Immunoematologia IRCCS Ospedale Bambino Gesù, sede di Roma.
(°) Azienda S. Andrea, II Facoltà di Medicina e Chirurgia dell’Università degli Studi di Roma “La Sapienza”
La nostra esperienza evidenzia l’utilità del gel piastrinico (GP) nel trattamento ambulatoriale delle piaghe torpide della pianta del piede in paziente diabetico. Il GP guarisce in tempi prevedibili ulcerazioni torpide che richiedevano lunghissimi periodi di medicazioni complesse senza raggiungere il risultato prefissato. La nostra esperienza, analogamente ai risultati di altri autori, depone favorevolmente per questo trattamento. La metodica personalmente messa a punto prevede il curettage dopo disinfezione, l’inoculazione di 1 UI di insulina pronta alla base del cratere ulcerativo e quindi l’applicazione di 5 ml di GP con 1 ml di Trombina autologa attivata. L’applicazione può essere eseguita mediante un puntale spray o cannula a seconda delle esigenze dell’operatore o del tipo di lesione. In caso di ferite più piccole o man mano che la ferita in trattamento si riduce di ampiezza, il prodotto può essere aliquotato e congelato per successive somministrazioni. Il GP e la trombina vanno spalmati in situ e su un supporto inerte riassorbibile di acido ialuronico (Hialogel) con il quale si zaffa la cavità e che impedisce la dispersione della parte liquida e non completamente gelificabile, ricca dei fattori di crescita (PDGF, TGF, EGF, ed altri). Il rapporto strettamente collaborativo tra dermatologo, chirurgo, ortopedico, immunotrasfusionista, diabetologo e cardiologo, risolve questa problematica con un approccio multidisciplinare. Lo stretto coordinamento tra centro trasfusionale ed ambulatorio chirurgico evita inutili attese al Paziente, accorcia i tempi di esecuzione della medicazione, abbatte i costi per l’impiego di materiali e alla lunga diventa conveniente rispetto al tradizionale trattamento.Our study proves the usefulness of platelet gel in the treatment of the diabetic foot. We started in January 2006 to treat diabetic wounds of the foot in the outpatients’ surgical department with encouraging results. Despite its expensive and complex preparation, the platelet gel is useful and convenient because it succeeds in shortening the ambulatory treatment period. Besides, in our opinion, the multidisciplinary approach of this treatment is rather important: actually, it implies the cooperation of dermatologist, surgeon, orthopaedist, immunologist, diabetologist and, if necessary, the cardiologist. That is why it reduces wastes of work-time and the expenses for consultants, medications and dressing material
Silicon nitride and oxynitride films deposited from organosilicon plasmas: ToF-SIMS characterization with multivariate analysis
No full textIn this work bis(dimethylamino)dimethylsilane (BDMADMS) has been utilized as precursor for plasma enhanced chemical vapour deposition
(PECVD) of silicon nitride-like and silicon oxynitride-like thin films. The effect of input power and O2/BDMADMS ratio on the film surface
chemistry has been investigated by time-of-flight secondary ion mass spectrometry (ToF–SIMS) and principal component analysis (PCA).
Changes in surface composition have been correlated to infrared (FT-IR) and X-ray photoelectron spectroscopy (XPS) analyses. The results show
that the PCs profile obtained by increasing input power during the deposition is correlated to the transition from an organic to an inorganic silicon
nitride-like coating while at high input power, if the oxygen concentration in the feed is increased, the following transition occurs: inorganic silicon
nitride-like→oxynitride-like→oxide-like
Slabs of stone material, resistant to wear, to corrosion caused by acids and to the staining action of oily substances
No full textEvaluation of Serum Apolipoprotein A1 in Canine Sepsis
No full textDecreased serum apolipoprotein A1 (Apo-A1) concentration is associated with mortality in human sepsis. The diagnostic and prognostic role of serum Apo-A1 concentrations in canine sepsis was evaluated. Serum samples from septic dogs (n = 91) and healthy controls (n = 15) were retrospectively analyzed. According to the sepsis origin, four categories were identified: parvoviral enteritis (n = 26), pyometra (n = 20), septic peritonitis (n = 19), and miscellanea (n = 26). The canine acute patient physiologic and laboratory evaluation fast score (APPLEfast), serum C-reactive protein (CRP) and albumin concentrations were reviewed in all enrolled dogs. Increased CRP (252.6 ± 119.2 mg/L; Reference Interval: 0–8.5 mg/L) and significant lower serum albumin and Apo-A1 concentrations were documented in dogs with sepsis (22.8 ± 5.3 g/L and 1.17 ± 0.27 g/L, respectively) compared to healthy ones (33.1 ± 2.5 g/L and 1.32 ± 0.05 g/L, respectively) (P < 0.0001). According to the origin of sepsis, only the subgroup of dogs with septic peritonitis had significantly lower Apo-A1 (1.03 ± 0.26 g/L) concentrations compared to healthy dogs (P < 0.001). No significant differences were found in serum albumin and CRP concentrations, and in APPLEfast score values among the different subgroups of sepsis. Diagnosis of septic peritonitis was associated with a higher frequency of death (P = 0.006). In septic dogs, significant lower Apo-A1 concentrations were detected in non-survivors (1.02 ± 0.28 g/L; n = 27) compared to survivors (1.23 ± 0.24 g/L; n = 64; P = 0.0007). Moreover, significant higher values of the APPLEfast score were calculated in non-survivors (26 ± 4; n = 19) compared to survivors (23 ± 4; n = 51) (P = 0.0114). According to the area under the ROC curve analysis, Apo-A1 <96 mg/dl had a fair accuracy (AUC = 0.72) to correctly predict mortality (P = 0.0004). Apo-A1 might support a diagnosis of canine septic peritonitis with a potential prognostic significance. Further prospective studies are warranted
ACE2 Expression in the Cat and the Tiger Gastrointestinal Tracts
No full textAngiotensin-converting enzyme 2 (ACE2) has been identified as the functional receptor for Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2). It has been identified in the human gastrointestinal tract (GIT), and SARS-CoV-2 has been isolated in human and animal fecal samples. The aim of the present study was to investigate the expression of ACE2 in the gastrointestinal tract of domestic (cat) and wild (tiger) felines. Samples of the pylorus, duodenum, and distal colon were collected from six cats and one tiger. The tissues were processed for immunofluorescence assay with an anti-human ACE2 antibody. Angiotensin-converting enzyme 2 was widely expressed in the gastrointestinal mucosa of the cats and the tiger. In both the species, ACE2-immunoreactivity (ACE2-IR) was expressed by the mucosal epithelial cells of the GIT and by the enteric neurons. In the cats, ACE2-IR was also expressed by the smooth muscle cells of the blood vessels and the tunica muscularis. The expression of the ACE2 receptor in enteric neurons may support the potential neurotropic properties of SARS-CoV-2. Although the evidence of ACE2-IR in the feline GIT does not necessarily indicate the possibility of viral replication and SARS-CoV-2 spread with stool, the findings in the present study could serve as an anatomical basis for additional studies considering the risk of the SARS-CoV-2 fecal-oral transmission between cats/felids, and between cats/felids and humans
Shape optimization and uncertainty assessment of a centrifugal pump
No full textCentrifugal pumps, being used nowadays for many applications, must be suited for a wide range of pressure ratios and flow rates. To overcome difficulties arising from the design and performance prediction of this class of turbomachinery, many researchers have proposed coupling Computation Fluid Dynamics (CFD) codes and optimization algorithms for fast and effective design procedures. However, uncertainties are present in most engineering applications such as turbomachines, and their influence on performance should be assessed. In this work, some advanced optimization techniques are applied to the blade optimization of a European Research Community on Flow, Turbulence and Combustion (ERCOFTAC)-like pump, and the robustness of the optimal profiles through an uncertainty quantification study is assessed. Two primary sources of uncertainty are considered: (i) equipment uncertainties, primarily the rotational speed of the pump shaft that also affects the flow rate; and (ii) uncertainties in the inlet turbulence quantities in CFD simulations
Thin film deposition in Glow Discharges fed with hexamethyldisilazane-O2 mixtures
No full textThin films deposited in glow discharges fed with hexamethyl disilazane-oxygen-argon mixtures at low substrate temperature have been studied by means of in situ x-ray photoelectron spectroscopy. The results have been compared with those obtained with tetraethoxysilane-oxygen plasmas. It has been found that hexamethyldisilazane, even if less oxidable, is strongly fragmented in the plasma phase. The oxygen atoms produced by plasma dissociation promote decrease of nitrogen, carbon and hydrogen content of the film but are not able to form oxygenated nitrogen compounds. The results are in agreement with a deposition mechanism composed of different gas-gas and gas-surface reactions as previously found for tetraethoxysilane. The main difference is that in the present work the homogeneous activation reaction of unreacted compound by means of oxygen atoms is important even when the oxygen content of the feed is low. When the oxygen content of the film is high, the chemical composition of the film surface is slightly different from that of the bulk
Uncertainty Assessment of an Optimized ERCOFTAC Pump
No full textCentrifugal pumps, being used nowadays for many applications, must be suited for a wide range of pressure ratios and flow rates. To overcome difficulties arising from the design and performance prediction of this class of turbomachinery, many researchers proposed the coupling of CFD codes and optimization algorithms for a fast and effective design procedure. However, uncertainties are present in most engineering applications such as turbomachines, and their influence on turbomachinery performance should be considered. In this work we apply some advanced optimization techniques to the blade optimization of an ERCOFTAC-like pump, and we assess the robustness of the optimal profiles through an uncertainty propagation study. The main sources of uncertainty are related to the operating conditions, primarily the rotational speed of the pump shaft that affects also the flow rate
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