1,757,195 research outputs found
Fn-Dps enhances the intracellular survival of Fn in macrophages.
No full textRAW264.7 cells were infected with Fn or heat-killed Fn (K-Fn) at an MOI of 10:1 (bacteria: cells). (A) Intracellular and extracellular Fn-Dps levels in RAW264.7 cells. (B) RAW264.7 cells were infected with K-Fn, Fn, or Fn-Dps (1.0 μM) (Fn+ Fn-Dps). (C) Colony forming units (CFUs) in RAW264.7 cell lysates. (D) Fn-infected RAW264.7 cells with or without NC-siRNA (50 nM), si-CCL2 (50 nM), si-CCL7 (50 nM) or si-CCL2/7 (50 nM) + Fn-Dps (1.0 μM). (E) Colony forming units (CFUs) in RAW264.7 cell lysates. Scale bar = 50 μm. Data are expressed as mean ± SD and compared by Student’s t test (C and E). *PP<0.01. n = 3 independent experiments.</p
Fn-Dps promotes the migration of CT26 cells.
No full text(A) Schematic diagram of the CT26 cells migration model: (a) CT26 cells were seeded in the top well, and RAW264.7 or J774A.1 cells were added to the lower wells treated with PBS (control), (b) or treated with Fn-Dps, (c) or treated with CCL2, (d) or treated with CCL7, (e) or treated with CCL2+CCL7, (f) or treated with CCL2/7 nAb+ Fn-Dps. Representative images of the assay (right). (B) The migration of CT26 cells was assessed using Transwell migration assays. Representative images of the assay (right). (C) Analysis of CT26 cells migration by scratch assays. CT26 cells were treated with supernatant of RAW264.7 or J774A.1 cells alone (control), with CCL2, with CCL7, with CCL2+CCL7, or with supernatant of RAW264.7 or J774A.1 cells treated with CCL2/7nAb + Fn-Dps. Scratch area was recorded after treatment for 48 h. Representative images of the assay (right). (D) CT26 cells were treated with supernatant of RAW264.7 or J774A.1 cells alone (control), with CCL2, with CCL7, with CCL2+CCL7, or with supernatant of RAW264.7 or J774A.1 cells treated with CCL2/7nAb+Fn-Dps for 48 h. The expression of E-cadherin, N-cadherin, Snail, and Vimentin was measured by Western blot analysis. Scale bar = 200 μm. Data are expressed as mean ± SD and compared by Student’s t test (B and C). *PPP <0.001. n = 3 independent experiments.</p
Fn-Dps promotes the migration of RKO cells.
No full text(A) Schematic diagram of the RKO cells migration model: (a) RKO cells were seeded in the top well, and macrophages derived from THP-1 cells (M-THP-1) were added to the lower wells treated with PBS (control), (b) or treated with Fn-Dps, (c) or treated with CCL2, (d) or treated with CCL7, (e) or treated with CCL2+CCL7, (f) or treated with CCL2/7 nAb+Fn-Dps. Representative images of the assay (right). (B) The migration of RKO cells was assessed using Transwell migration assays. Representative images of the assay (right). (C) Analysis of RKO cells migration by scratch assays. RKO cells were treated with supernatant of M-Thp-1cells alone (control), with CCL2, with CCL7, with CCL2+CCL7, or with supernatant of M-Thp-1 cells treated with CCL2/7nAb+Fn-Dps. Scratch area was recorded after treatment for 48 h. Representative images of the assay (right). (D) RKO cells were treated with supernatant of M-Thp-1 cells alone (control), with CCL2, with CCL7, with CCL2+CCL7, or with supernatant of M-Thp-1 cells treated with CCL2/7nAb+Fn-Dps for 48 h. The expression of E-cadherin, N-cadherin, Snail and Vimentin was measured by Western blot analysis. Scale bar = 200 μm. Data are expressed as mean ± SD and compared by Student’s t test (B and C). *PPP (PDF)</p
Fn-Dps stimulated the expression of CCL2/CCL7 in RAW264.7 cells.
No full text(A) The CCK-8 assay was used to detect the effects of Fn-Dps (1.0 μM) on RAW264.7 cell proliferation at the indicated times. (B) Morphological changes of RAW264.7 cells stimulated by Fn-Dps (1.0, 10 μM). Scale bar = 400 μm. (C) Heatmap based on the top 21 upregulated miRNAs between PBS-treated RAW264.7 cells (control) and 1.0 μM Fn-Dps-treated RAW264.7 cells. (red, upregulation; blue, downregulation). (D) Heatmap based on the top 22 upregulated miRNAs between PBS-treated RAW264.7 cells (control) and Fn-infected RAW264.7 cells. (red: upregulation, blue: downregulation). (E) Venn diagram illustrating the relationship between differentially expressed mRNAs in the two discrete comparisons: Control vs. Fn (red circle) and Control vs. Fn-Dps (blue circle). (F) Differentially expressed genes were validated by RT–qPCR. (G) ELISA assay detecting CCL2/CCL7 levels after1.0 μM Fn-Dps-treated RAW264.7 and J774A.1 cells. Scale bar = 100 μm. Data are expressed as mean ± SD and compared by One-way ANOVA test (A) or Student’s t test (F and G). *PP <0.001. n = 3 independent experiments.</p
Serum anti-Fn-Dps antibody levels in CRC patients and healthy subjects.
No full textSerum anti-Fn-Dps IgG (A) and anti-Fn-Dps IgA (B) were detected by Western blotting in healthy subjects (H1–8) and Fn-positive CRC patients (C1–8). Comparison of antibody levels of anti-Fn-Dps-IgG (C) and IgA (D) in healthy subjects (HS, n = 144) and total CRC patients (n = 123). Comparison of antibody levels of anti-Fn-Dps-IgG (E) and IgA (F) in stage I CRC patients (n = 27) and stage II-IV CRC patients (n = 96). Correlation between serum anti-Fn-Dps IgG (G), anti-Fn-Dps IgA (H), and anti-Fn in CRC patients. Data are expressed as mean ± SD and compared by the Wilcoxon test (C, D, E, and F) or Pearson correlation test (G and H). *PPP <0.001.</p
Project 17B: Bern FN Core: Pediatric patients at risk for fever in chemotherapy-induced neutropenia (FN) in Bern, Switzerland, 1993-2012
No full textCohort of 583 pediatric patients treated with chemotherapy for cancer in Bern, Switzerland, from 1993 to 2012. 2113 observation periods covering 692 years of chemotherapy exposure with 712 FN episodes, 154 of them FN with bacteremia.
Dataset usable, e.g., for risk prediction of FN, and assessment of long-term changes of risk factors, FN rates, and their associations (interaction with time)
Introduction: Technology and International Relations – The New Frontier in Global Power
No full textThe chapter introduces how changes in advanced technology deeply affect international politics, this book theoretically engages with the overriding relevance of investments in technological research, and the ways in which they directly foster a country’s economic and military standing. The scholars and practitioners in the volume present important insights on the technical and social issues at the core of technology competition. The Introduction to Technology and International Relations emphasizes the importance of leadership styles, domestic political agendas and the relative weight of technologically driven countries in global affairs. It highlights the now widely shared belief among both developed and developing countries that technology will be the defining factor in international politics. The book also unpacks the complexity of real-life cases of key technological advances, including artificial intelligence, UAVs, satellites and the responses of governments and the private sector to rising technological challenges
Project 17C: Bern FN Core: Episodes of fever in chemotherapy-induced neutropenia (FN) in pediatric patients in Bern, Switzerland, 1993-2012
No full textData on potential risk factors and on outcomes in 703 FN episodes diagnosed in 291 of 583 pediatric patients treated with chemotherapy for cancer in Bern, Switzerland, from 1993 to 2012.
These data can mainly be used (1) to study FN characteristics over time and between centers; (2) to study the association of FN characteristics with outcomes over time and between centers; (3) to derive corresponding CDRs for risk-adapted treatment of FN in pediatric patients; and (4) to externally validate CDRs derived from other datasets
FN-RE: A Corpus of Requirements Documents Enriched with Semantic Frame Annotations
No full text<p>FN-RE is a human-labelled dataset using FrameNet scheme. The dataset is distributed and can be viewed using a web-index page. For further details about the annotation procedures, please refer to the annotation guidelines included in the folder.</p>1/ Download the 'FN-RE-Corpus (RE-DATA Track)' folder into your preferable directory, without moving or changing the internal folders and files.
2/ Open FN-RE-Corpus-Access-Interface.html to navigate FN-RE corpus (we recommend using Firefox web browser)
DataSheet_1_Eliminating the invading extracellular and intracellular FnBp+ bacteria from respiratory epithelial cells by autophagy mediated through FnBp-Fn-Integrin α5β1 axis.docx
No full textBackgroundWe previously found that the respiratory epithelial cells could eliminate the invaded group A streptococcus (GAS) through autophagy induced by binding a fibronectin (Fn) binding protein (FnBp) expressed on the surface of GAS to plasma protein Fn and its receptor integrin α5β1 of epithelial cells. Is autophagy initiated by FnBp+ bacteria via FnBp-Fn-Integrin α5β1 axis a common event in respiratory epithelial cells?MethodsWe chose Staphylococcus aureus (S. aureus/S. a) and Listeria monocytogenes (L. monocytogenes/L. m) as representatives of extracellular and intracellular FnBp+ bacteria, respectively. The FnBp of them was purified and the protein function was confirmed by western blot, viable bacteria count, confocal and pull-down. The key molecule downstream of the action axis was detected by IP, mass spectrometry and bio-informatics analysis.ResultsWe found that different FnBp from both S. aureus and L. monocytogenes could initiate autophagy through FnBp-Fn-integrin α5β1 axis and this could be considered a universal event, by which host tries to remove invading bacteria from epithelial cells. Importantly, we firstly reported that S100A8, as a key molecule downstream of integrin β1 chain, is highly expressed upon activation of integrin α5β1, which in turn up-regulates autophagy.ConclusionsVarious FnBp from FnBp+ bacteria have the ability to initiate autophagy via FnBp-Fn-Integrin α5β1 axis to promote the removal of invading bacteria from epithelial cells in the presence of fewer invaders. S100A8 is a key molecule downstream of Integrin α5β1 in this autophagy pathway.</p
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