1,721,042 research outputs found
Fibrin gels loaded with cisplatin and cisplatin-hyaluronate complexes tested in a subcutaneous human melanoma model
No full textSummary Fibrin gels are attractive biomaterials for local delivery
of a variety of agents, from drugs to proteins. Similarly,
polymer-anticancer-drug conjugates and nanoparticles are
emerging as potential candidates for cancer treatment.
Combining these different approaches, we have studied the
efficacy of fibrin gels loaded with cisplatin (DDP) and a complex
of DDP with hyaluronate (DDP-HA) for tumor growth
inhibition in a melanoma model. Loaded gels prepared at relatively
high fibrinogen concentration (22 mg/ml) showed
good in vitro antiproliferative activities, prolonged release of
the anticancer drug, and a long persistence (10–15 days)
in vivo when implanted subcutaneously (sc) in immunodeficient
mice. Gels loaded with DDP or DDP-HA containing 1/3
or even 1/6 of their systemic dose (6 mg/kg) and positioned
under the tumor mass in mice bearing a sc human SK-Mel-28
tumor showed an antitumor activity better than that of the
original parent compound given intraperitoneally (ip).
Moreover, in an additional experiment in vivo, fibrin gels
loadedwith N-trimethyl chitosan-based nanoparticles containing
a DDP-HA complex were assayed, resulting in a further
8 % improvement of anticancer activity, with lesser adverse
systemic toxic effects. Taken together, these results suggest
that the combination of fibrin gels and drugs complexed with
suitable macromolecules holds great promise for locoregional
anticancer therapy of melanoma and other surgically
removable cancer types
Frog hepatocyte modifications induced by seasonal variations: a morphological and cytochemical study
No full textSeasonal variation of K+-p-nitrophenyl phosphatase activity in the epidermis of the crested newt: a quantitative and ultrastructural study.
No full textUltrastructural patterns of the a-naphthyl butyrate esterase activity in normal and leukaemic peripheral blood leukocytes.
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Cytochemical demonstration of rat liver adenyl cyclase. Attempts of methodological improvements.
No full textCytochemical study of K+-dependent p-nitrophenyl-phosphatase activity in the urinary bladder of Salamandra salamandra.
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