1,721,050 research outputs found
Monografia di Lorenzo Fassina: "Hybrid bone design: a tissue-engineering approach through biomaterials and bioreactors"
No full textArticolo di Lorenzo Fassina: "L'Ingegneria Tissutale" sulla rivista “Prevenzione Cardiovascolare” (Organo di informazione dell’ANPC - Associazione Nazionale Prevenzione Cardiovascolare)
No full textEditorial: Cells, Biomaterials, and Biophysical Stimuli for Bone, Cartilage, and Muscle Regeneration
No full textVisai, Livia/0000-0003-1181-3632; Fassina, Lorenzo/0000-0002-5587-4632; Ramalingam, Murugan/0000-0001-6498-9792; Cusella De Angelis, Maria Gabriella/0000-0003-2642-3346[No Abstract Available
Numerical dosimetry inside an extremely-low-frequency electromagnetic bioreactor
Full text linkWe have implemented field models and performed a detailed numerical dosimetry inside our extremely-low-frequency electromagnetic bioreactor. The numerical dosimetry permitted to map the magnetic induction field (maximum module equal to about 3.3 mT) and to discuss its biological effects in terms of induced electric current and induced biophysical forces (compression and traction)
Physically enhanced surface modification of titanium plasma-spray with human SAOS-2 osteoblasts and extracellular matrix
No full text“Non per noi, ma per tutti” dissero i riders. E così fu.
No full textNel contributo, l'A. sottolinea come che l'art. 2, d.lgs. n. 81/2015, come modificato dalla legge n. 128/2019, abbia un campo d’applicazione significativamente più ampio del delivery-food e, per vero, anche più esteso del lavoro reso mediante piattaforme digitali. Per l'A. nn pare revocabile in dubbio che la ratio legis sia orientata ad estendere la platea dei beneficiari del meccanismo prospettato col Jobs Act: quando una fattispecie perde alcuni requisiti come il riferimento al “tempo” e “luogo” di lavoro, che avevano se non autorizzato per lo meno facilitato o reso credibile per qualcuno (v. Trib. Torino) un’interpretazione molto restrittiva della disposizione derubricata a “norma apparente” (Tosi, 2015); quando all’esclusiva personalità della prestazione si sostituisce la “prevalente personalità della prestazione”, adottando una soluzione coerente con le fattispecie di lavoro autonomo (artt. 2222 c.c. e art. 409, n. 3, c.p.c.), nel cui alveo sono ritagliate le collaborazioni etero-organizzate, è evidente l’intenzione di allargare al segmento del lavoro autonomo più prossimo al confine con la subordinazione l’integrale disciplina del lavoro subordinato
Physically augmented coating of macrorough titanium surface with human SAOS-2 osteoblasts and bone matrix
No full text
Video evaluation of kinematics and dynamics of the beating cardiac syncytium: an alternative to the Langendorff's method
No full textMany important observations and discoveries in heart physiology have been made possible using the isolated heart method of Langendorff. Nevertheless, the Langendorff’s method has some limitations and disadvantages such as the vulnerability of the excised heart to contusions and injuries, the probability of preconditioning during instrumentation, the possibility to induce tissue oedema, and a high oxidative stress, leading to the deterioration of the contractile function. To avoid the preceding drawbacks associated to the use of a whole heart, we have alternatively used beating mouse cardiac syncytia cultured in vitro in order to assess possible ergotropic, chronotropic, and inotropic effects of drugs.
To achieve the preceding aim, we have developed a method based on image processing analysis to evaluate the kinematics and the dynamics of that drug-stimulated beating syncytia starting from the video registration of their contraction movement. In this manner, in comparison with the physiological no-drug condition, we have observed progressive positive ergotropic, positive chronotropic, and positive inotropic effects of 10 microM isoproterenol (beta-adrenergic agonist) and early positive ergotropic, negative chronotropic, and positive inotropic effects of 10 microM phenylephrine (alpha-adrenergic agonist), followed by a late phase with negative ergotropic, positive chronotropic, and negative inotropic trends.
The present method permitted a systematic study of in vitro beating syncytia, producing results coherent with previous works. As consequence, it could be used in in vitro studies of beating cardiac patches, as alternative to the Langendorff’s heart in biochemical and pharmacological studies, and, especially, when the Langendorff’s technique is inapplicable (e.g., in studies about human cardiac syncytium in physiological and pathological conditions, patient-tailored therapeutics, and syncytium models derived from induced pluripotent/embryonic stem cells with genetic mutations).
Furthermore, the method could help, in heart tissue engineering and bioartificial heart researches, to “engineer the heart piece by piece”. In particular, the proposed method could be useful in the identification of a suitable cell source, in the development and test of “smart” biomaterials, and in the design and use of novel bioreactors and microperfusion systems
Potential of electromagnetic and ultrasound stimulations for bone regeneration
No full textGelatin-based cryogels have been seeded with human SAOS-2
osteoblasts. In order to overcome the drawbacks associated with in vitro
culture systems, such as limited diffusion and inhomogeneous cell–matrix
distribution, we have described the application of electromagnetic and
ultrasound stimulation to physically enhance the cell culture in vitro. The
results indicate that the physical stimulation of cell-seeded gelatin-based
cryogels upregulates the bone matrix production
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