826 research outputs found
Isotachophoresis
No full textIn this paper the main principles of isotachophoretic techniques are dealt with. Some examples of electropherograms, showing both cation and anion analyses, are given. Suggestions are made for the separation in solvents different from water. Various block diagrams of equipments, in which the analyses can be carried out, are shown
Carboxylic acids
No full textThis chapter discusses carboxylic acids that generally have high mobilities compared with those of proteins. Carboxylic acids used as model substances for checking both theoretical aspects and correct functioning of the equipment are separated qualitatively and quantitatively by isotachophoresis. Most electrophoretic work, except isotachophoresis is performed in equipment in which stabilizing media can be applied. Common isotachophoretic equipment makes use of narrow bore PTFE tubes with inside diameters of 0.2–0.6 mm. The separation of carboxylic acids qualitatively and quantitatively via moving boundary electrophoresis or ion focusing is less efficient in comparison to isotachophoresis. The analyses can be performed without sample pre-treatment in the column-coupling system or with a simple extraction and/or centrifugation. With the column-coupling system ionic substances at low concentrations in complex mixtures can be directly determined qualitatively and quantitatively. The urate-albumin complex is unstable under isotachophoretic conditions
Isoelectric focusing
No full textIsoelectric focusing, J.P. Arbuthnott, J.A. Beeley (Eds.), Butterworths, London (1975), XI + 367 pp., price £ 14.45, ISBN 0-408-70659-
Elektroforese, een scheidingstechniek alleen voor eiwitten?
No full textElektroforese is een verzamelnaam voor een aantal scheidingstechnieken (analytisch en
preparatief), die gebruikt worden om zowellaagmoleculaire als hoogmoleculaire stoffen, in
oplossing, kwalitatief en kwantitatief te analyseren. Daar de scheiding plaats vindt onder invloed van een elektrisch veld, moeten de te scheiden stoffen een elektrische lading hebben
- …
