1,733 research outputs found
La Shoah di Vanni Rossi (1946-1948)
No full textIl contributo esamina analiticamente la pittura muraria eseguita da Vanni Rossi nella chiesa milanese dei Santi Nereo e Achilleo nel 1948
Cellule staminali nell'ascidia coloniale Botryllus schlosseri: caratterizzazione di nicchie, fattori di staminalità e dinamiche di differenziamento.
No full textBotryllus schlosseri is a cosmopolitan colonial ascidian pertaining to tunicates, the sister group of vertebrates. It can regenerate entire organisms from a pool of circulating cells when all zooids are removed from a colony. Moreover, it possesses asexual reproduction, in which new individuals continuously develop, while old individuals are resorbed. The close relationship with vertebrates, the constant replacement of old individuals by newly formed zooid generations, together with the high regenerative abilities characterizing this species, make this organism a captivating model for the study of stemness that is the focus of my PhD project.
During my PhD, I submitted two book chapters. A first one concerns the methodologies used to study regeneration in colonial and solitary ascidians, since the end of the 19th century until nowadays (Methodological approaches for the study of regeneration in ascidians: an historical overview. Vanni V, Ballarin L, Gasparini F, Peronato A, Manni L (2022). Whole-Body Regeneration: Methods and Protocols. Methods in Molecular Biology, Springer). The long history of studies in ascidians highlights how these organisms are easy to maintain in laboratory conditions, manipulate and follow in vivo. Indeed, the methodologies to induce the regeneration processes have not changed so much, as they are simple, low cost, and effective. However, even if many molecular and cellular tools are still missing for studying these organisms, by applying modern techniques, it has been possible, in recent years, to uncover some features characterizing ascidian stem cells. This is the subject of the second book chapter (Current Knowledge on Stem Cells in Ascidians. Vanni V, Anselmi C, Ballarin L, Drago L, Gasparini F, Gordon T, Peronato A, Rosental B, Rosner A, Rinkevich B, Spagnuolo A, Manni L, Voskoboynik A (2022). Advances in aquatic invertebrate stem cell research, MDPI).
Moreover, I followed three different lines to better characterize stemness in B. schlosseri, which are included in different articles.
In a paper in collaboration with Prof. Carmela Gissi (Università di Bari, IT), currently under revision, we verified a possible common expression pattern between vertebrate embryonic stem cells and adult candidate stem cells of B. schlosseri. We carefully retrieved the orthologous in B. schlosseri Yamanaka Factors (Myc, Pou2, Pou3 and SoxB1), and studied, through in situ hybridization, the expression of these genes during all the phases of the asexual cycle of this colonial ascidian. Our results highlight a conserved expression pattern of myc, pou3 and soxB1 in undifferentiated, developing territories, consistently with the expression pattern observed in other chordate species. Moreover, the genes are expressed in small round cells (candidate stem cells) located in the stem cell niches identified until now in B. schlosseri, suggesting that they may play a role in stemness.
In another paper (in preparation), we characterized the stem cell niches identified until now in B. schlosseri, by 3D reconstruction and histology, showing for the first time their cell structure and anatomy. We then investigated the contribution of candidate stem cells to the development of primary and secondary buds, confirming their infiltration in previously hypothesized sites, but also demonstrating that they can contribute to the development of organs and tissues previously not individuated.
Finally, a last paper (in preparation in collaboration with Prof. Jordi Solana, Oxford Brookes University, UK), regards the single cell transcriptomics of B. schlosseri: we successfully produced an atlas of 37 clustered cell populations, validated through published datasets and expressing markers for specific tissues. This atlas will be the basis for future analysis on stemness and differentiation in this organism.
Altogether, with the results obtained during my PhD, we advanced the knowledge on stem cells of B. schlosseri.Botryllus schlosseri is a cosmopolitan colonial ascidian pertaining to tunicates, the sister group of vertebrates. It can regenerate entire organisms from a pool of circulating cells when all zooids are removed from a colony. Moreover, it possesses asexual reproduction, in which new individuals continuously develop, while old individuals are resorbed. The close relationship with vertebrates, the constant replacement of old individuals by newly formed zooid generations, together with the high regenerative abilities characterizing this species, make this organism a captivating model for the study of stemness that is the focus of my PhD project.
During my PhD, I submitted two book chapters. A first one concerns the methodologies used to study regeneration in colonial and solitary ascidians, since the end of the 19th century until nowadays (Methodological approaches for the study of regeneration in ascidians: an historical overview. Vanni V, Ballarin L, Gasparini F, Peronato A, Manni L (2022). Whole-Body Regeneration: Methods and Protocols. Methods in Molecular Biology, Springer). The long history of studies in ascidians highlights how these organisms are easy to maintain in laboratory conditions, manipulate and follow in vivo. Indeed, the methodologies to induce the regeneration processes have not changed so much, as they are simple, low cost, and effective. However, even if many molecular and cellular tools are still missing for studying these organisms, by applying modern techniques, it has been possible, in recent years, to uncover some features characterizing ascidian stem cells. This is the subject of the second book chapter (Current Knowledge on Stem Cells in Ascidians. Vanni V, Anselmi C, Ballarin L, Drago L, Gasparini F, Gordon T, Peronato A, Rosental B, Rosner A, Rinkevich B, Spagnuolo A, Manni L, Voskoboynik A (2022). Advances in aquatic invertebrate stem cell research, MDPI).
Moreover, I followed three different lines to better characterize stemness in B. schlosseri, which are included in different articles.
In a paper in collaboration with Prof. Carmela Gissi (Università di Bari, IT), currently under revision, we verified a possible common expression pattern between vertebrate embryonic stem cells and adult candidate stem cells of B. schlosseri. We carefully retrieved the orthologous in B. schlosseri Yamanaka Factors (Myc, Pou2, Pou3 and SoxB1), and studied, through in situ hybridization, the expression of these genes during all the phases of the asexual cycle of this colonial ascidian. Our results highlight a conserved expression pattern of myc, pou3 and soxB1 in undifferentiated, developing territories, consistently with the expression pattern observed in other chordate species. Moreover, the genes are expressed in small round cells (candidate stem cells) located in the stem cell niches identified until now in B. schlosseri, suggesting that they may play a role in stemness.
In another paper (in preparation), we characterized the stem cell niches identified until now in B. schlosseri, by 3D reconstruction and histology, showing for the first time their cell structure and anatomy. We then investigated the contribution of candidate stem cells to the development of primary and secondary buds, confirming their infiltration in previously hypothesized sites, but also demonstrating that they can contribute to the development of organs and tissues previously not individuated.
Finally, a last paper (in preparation in collaboration with Prof. Jordi Solana, Oxford Brookes University, UK), regards the single cell transcriptomics of B. schlosseri: we successfully produced an atlas of 37 clustered cell populations, validated through published datasets and expressing markers for specific tissues. This atlas will be the basis for future analysis on stemness and differentiation in this organism.
Altogether, with the results obtained during my PhD, we advanced the knowledge on stem cells of B. schlosseri
Il consenso del minore al trattamento medico in prospettiva comparatistica : un nuovo soggetto di diritto?
No full textThe research analyzes in a comparative perspective the new role recognized to the minor thanks to the juridical relevance of his will with particular reference to some recent jurisprudential rulings both of the Suprême Court, in the English system, and of the Court of Cassation, in the Italian law. In this new perspective, the minor is increasingly considered the centre of imputation of rights and duties and less perceived as a weak subject, whose protection is traditionally entrusted to parents or, in case of their absence, to other substitute figures. In this new approach, the minor seems to play a new juridical subjectivity and his will must be increasingly taken into consideration as it represents the starting point of any decision that concerns him, especially with regard to decisive choices for his own life, such as in the case of informed consent to medical treatment. From a comparative perspective, a new conception of the minor, no longer seems to appear in the private sector - and in particular in English and Italian legal systems, which take into consideration the best interest of the child, but a real subject of law, author of his own destiny. This approach is based on two key points: 1. the fundamental principle of non-discrimination, reaffirmed both internationally and nationally in the examined legal systems (English and Italian) with a particular reference - in addition to gender, race, language and religion - to age; 2. the minor as a subject of law through the recognition of the juridical relevance of his will in previously unthinkable areas, including the sensitive sphere of health treatment. 
Degree-correlations in a bursting dynamic network model
No full textWe propose a mathematical description of a dynamic network model in which the number of links fluctuates over time according to the degree-preferences of nodes. More specifically, we consider the minimal case of a bipartite directed network where we have two groups of nodes, each group has nodes with a given capability to bear links. One group is composed of nodes that create as many links as possible, the generators. The other group is composed of nodes which delete as many links as possible, i.e., the destroyers. We provide here a novel analytical formulation of the evolution of the system through coupled master equations for the two interacting populations, recovering the steady state degree distributions and a new analytic description of the transient dynamics to the equilibrium. Further, fluctuations are shown to be connected to a peak in degree correlation at a critical point of the system corresponding to equal-size populations of generators and destroyers. We investigate the nature of the neighbor connectivity and the temporal assortativity of the network, noticing that degree correlation are anomalously large at criticality and that they are not a pointwise characterization of the system in time but they emerge as an aggregate temporal property. Moreover, we examine the bursty dynamics of the network as a temporal property where the system heterogeneously evolves over time alternating between periods of low and high connectivity displaying a heavy-tailed distribution in the inter-event times distributions. Finally, we introduce a generalization of the model in which intermittent states can control the velocity of the network’s evolution. We will also provide examples of possible economic applications of the present network model
IL PROGETTO CUSTODIA DEL TERRITORIO
No full textIl capitolo descrive il progetto Custodia del Territorio nella Media Valle del Serchio (LU) attuato mediante convenzione tra Consorzio di Bonifica e agricoltori per la tutela e manutenzione del reticolo idraulico
A Study on the Symptomatology and Diagnostic Methodology of Vanni Pitham
No full textVanni Pitham a clinical entity was described by Sage Yugi in his wisdom. The study conducted has come out with excellent results validating the clinical features of Vanni Pitham elucidated in an ultra short poetic segment by Yugi. The study was aimed at evolving a set of exclusive Siddha diagnostic findings for Vanni Pitham with the observation and inference of various parameters like Naadi, Neikkuri and disease acquired season, it can be concluded that all of them point to the development or vitiation of humour leading to the disease Vanni Pitham. The patient reported with the symptoms of Vanni Pitham were subjected to the standard set of investigations, the results and findings of the investigations were suggestive of Vanni Pitham according to modern classification of disease. Manikkadai Nool and Nei kuri findings may help in the identifying of preponderance in a person to develop Vanni Pitham, hence it can be used as a screening measure to advise the preventive measures well in advance. Almost the patients who diagnosed as Vanni Pitham had significant study of colonoscopy evidence conforming to the correlation of disease with inflammatory bowel disease. From the analysis done between Vanni Pitham cases and control group, notable variations were observed in both Siddha and Modern parameters. Interestingly, it was found that the symptoms presented by the patients in the study were those of a constant subset of symptoms of inflammatory bowel disease explained in the present day classification. It correlated with all of the symptoms mentioned by Yugi muni under Vanni Pitham. Thus the author concludes by throwing lights on validation of symptomatology and exclusive Siddha diagnostic methodology for Vanni Pitham so that a physician can arrive at proper treatment procedures by rightly diagnosing the disease
Simulation of coalescence, break up and mass transfer in bubble columns by using the Conditional Quadrature Method of Moments in OpenFOAM
No full textThe evaluation of the mass transfer rates and the fluid-dynamics aspects of bubble columns are strongly affected by the intrinsic poly-dispersity of the gas phase, namely the different dispersed bubbles are usually distributed over a certain range of size and chemical composition values. In our previous work, gas-liquid systems were investigated by coupling Computational Fluid Dynamics with mono-variate population balance models (PBM) solved by using the quadrature method of moments (QMOM). Since mass transfer rates depend not only on bubble size, but also on bubble composition, the problem was subsequently extended to the solution of multi-variate PBM (Buffo et al. 2013). In this work, the conditional quadrature method of moments (CQMOM) is implemented in the open-source code OpenFOAM for describing bubble coalescence, breakage and mass transfer of a realistic partially aerated rectangular bubble column, experimentally investigated by Diaz et al.(2008). Eventually, the obtained results are here compared with the experimental data availabl
Consumo
No full textIl testo analizza l'evoluzione che i consumi hanno avuto in Italia dal Rinascimento agli anni Ottanta
La sorpresa como vicio de la voluntad
No full textFil: Vanni, Liliana Noemi. Universidad de Buenos Aires. Facultad de Derecho. Buenos Aires, ArgentinaDoctora de la Universidad de Buenos Aires en Derech
- …
