115 research outputs found
Isolation process for mitochondrial transplantation
No full textMitochondrial transplantation is an innovative therapeutic approach for the treatment of mitochondrial dysfunction associated with a variety of metabolic, neurodegenerative and cardiovascular diseases. In this procedure, healthy mitochondria are isolated and transferred into damaged or dysfunctional cells to restore normal cellular bioenergetics. Recent advances have shown the feasibility of mitochondrial transplantation in preclinical and clinical settings, demonstrating its potential to improve outcomes in conditions such as myocardial infarction, neurodegenerative disorders, and acute kidney injury. In this contest, the isolation method for mitochondrial transplantation is crucial. High quality, pure mitochondria are essential to ensure that the transplanted organelles function correctly and integrate well into the recipient cells. In addition, efficient isolation techniques are required to obtain a sufficient number of mitochondria for transplantation. Low yield methods may not provide enough mitochondria for effective treatment. Several isolation techniques are currently used, including differential centrifugation, density gradient centrifugation and fractionated mitochondrial magnetic separation. Each method has its own advantages and limitations, and the choice of method often depends on the specific requirements of the application, such as the type of cells targeted and the disease to be treated. A key factor to consider when choosing the isolation method is the isolation time. Rapid isolation methods are preferred for the isolation of mitochondria from tissue biopsies. This review summarizes the current state of research on mitochondrial transplantation, highlights the main methods for isolating mitochondria and discusses their potential clinical applications in the field of regenerative medicine
Storage of isolated mitochondria
No full textAfter extraction and quantification steps, the most intriguing challenge in preparing mitochondria for transplantation of these organelles is the preservation of the material isolated from different tissues. It is essential to maintain these organelles in a viable condition without losing the biological characteristics of the tissue of origin. After isolation and quantification, various methods, when performed following proper protocols, lead to the last step of preparation mitochondria ready for use. Two main methods are used for preservation of mitochondria after isolation: cold storage and cryopreservation. Current methodological approaches in mitochondrial transplantation techniques suggest using freshly prepared isolated mitochondria from different tissues; this could be a limitation for mitochondrial transplantation techniques, and further efforts in methodological research should be aimed at overcoming this problem
A COMPARISON STUDY FROM A CREATIVE THINKING PERSPECTIVE IN DIFFERENT DOMAINS AS ART EDUCATION AND NON-ART EDUCATION STUDENTS
No full textCreative thinking measurement for everyone in different domains is the most crucial critical subject. Therefore, the current discussion continues whether creativity in various fields different. This study aimed to measurement the students’ creative thinking scores in various domains disciplines to reveal differences. The main research question was as followed: Do whether students’ creative thinking scores of students in art and non-art education differ significantly? The method was a causal-comparative research design. Participant students (N = 456, meanage = 16–21) were equivalent regarding some variations; as education disciplines and ages. While however, the art and non-art education students were the domain-specific was art education students, the and domaingeneral, was non-art education students respectively. The present study determined a significant difference between domain-specific and domain-general on creative thinking scores in favor of the non-art education students in the range of ages 16 to 21. The study concluded that alternative instruments with domain-specific content needed to measure individuals’ creative thinking in the domain-specific. This study suggested future research to conduct creativity measurement comparatively individuals in different age ranges and domains fields. © 2023 The Author(s). Published by Vilnius Gediminas Technical University
Alloprevotella rava isolated from a mixed infection of an elderly patient with chronic mandibular osteomyelitis mimicking oral squamous cell carcinoma
No full textThe anaerobic, Gram-negative bacillus Alloprevotella rava has recently been described in the human oral cavity. To our knowledge, this species has not been isolated from chronic osteomyleitis samples. We present the first case of A. rava infection in a 92-year-old woman with polymicrobial chronic mandibular osteomyelitis, mimicking oral squamous cell carcinoma. (c) 2021 The Author(s). Published by Elsevier Ltd
Chronic hepatitis C responds poorly to combination therapy in chronic hepatitis B carriers
No full textBackground: The effect of conventional interferon-based therapy of hepatitis B virus (HBV) and hepatitis C virus (HCV) dual infection is controversial. Yet, no studies have been carried out into pegylated interferon treatment for chronic HBV/HCV coinfection. We aimed to evaluate the response rate and side effects of conventional or pegylated interferon combined with ribavirin on chronic HBV/HCV coinfection therapy. Methods: The study included 36 chronic hepatitis patients (M/F: 28/8, mean age 47 12 years) who were positive for HBsAg and anti-HCV. They were tested for the presence of HBV-DNA by hybridisation assay, and the samples giving negative results were retested by polymerase chain reaction (PCR). All patients were tested for HCV-RNA using PCR, and the HCV genotype was determined. Results: Nineteen patients were given standard interferon either alone or in combination with ribavirin, whereas 17 were given pegylated interferon and ribavirin combination therapy. None of the patients had HBV-DNA positivity; however, all had HCV-RNA detectable by PCR. All the patients had HCV genotype 1b. The mean alanine aminotransferase and aspartate aminotransferase levels were 118 +/- 65 U/l and 90 +/- 95 U/l respectively. Five patients in each group discontinued the treatment due to side effects. Only two patients (one from each group) reached sustained virological response. Conclusion: Neither pegylated nor conventional interferon based regimes were effective for HBV/HCV coinfection, in which the dominant virus was HCV. Pegylated interferon and ribavirin therapy was not superior to conventional interferon based regimes in the treatment of HBV/HCV coinfection
HTA REVIEW OF DECISIONS FOR PRODUCTS INDICATED IN THE TREATMENT OF SYSTEMIC LUPUS ERYTHEMATOSUS (SLE) AND LUPUS NEPHRITIS (N) IN UK, GERMANY, FRANCE, ITALY, SPAIN, CANADA AND TURKEY
No full textEvaluation of aortic stiffness in tobacco-smoking adolescents
No full textPurpose: To measure the aortic stiffness in tobacco-smoking adolescents and to investigate its relationship to tobacco smoke. Methods: Aortic strain (S), pressure strain elastic modulus (E-p), and normalized E-p (E-p*) in tobacco-smoking adolescents and the healthy control group were measured by a sphygmomanometer with cuff and transthoracic echocardiography. The study group consisted of 30 healthy cases (M/F: 27/3) as a control group and 30 tobacco-smoking volunteer adolescents (M/F: 28/2). Unpaired Student's t-test was used for comparison of these groups. Results: The mean ages were 16.1 +/- 1.8 years and 16.2 +/- 1.4 years, respectively. The number of cigarettes smoked per day was 31 +/- 7.1 and the duration of smoking was 3.4 +/- 1.1 years. S, E-p and E-p* measurements of tobacco smokers were different than the control groups' and this difference was statistically significant. S values were significantly higher in nonsmokers than in smokers; whereas E-p and E-p* values were significantly higher in smoker group. Conclusions: This study demonstrated that measurement of aortic stiffness with S, E-p, and E-p,* can be used as an early indicator of atherosclerosis in tobacco-smoking adolescents. (C) Society for Adolescent Medicine, 200
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