1,166 research outputs found
A hormone-dependent post-translationally regulated mutant for investigating type I cadherin function
No full textType I cadherins are Ca2+-dependent cell adhesion molecules. Their function in early Xenopus laevis development has been extensively studied in recent years, by injecting synthetic mRNAs encoding dominant negative mutants with deletions of the extracellular domain into embryos. However, studies at post-gastrula stages have been hampered by the inabilityto progress through post-gastrula development in embryos expressing these mutant proteins. This problem has been partly overcome by injecting into a few targeted blastomeres in stage 6 N.F. embryos, but only restricted studies are possible with this technique. Several studies have made use of the hormone-binding domain (HBD), which is activated by hormones. In this study, we used this method to analyze the activity of dominant negative cadherins. We generated a mutant E-cadherin (AE-Cad, consisting of the cytoplasmic domain and transmembrane domain) fused to the hormone-binding domain of estradiol receptor (HBDER) and we validated this technique with functional analyses. The function of the mutant deltaE-HBDER was strictly dependent on hormone induction. This conditional mutant had the same effects and exerted the same dominant negative function as the corresponding constitutive mutant
Gender in Schule und Didaktik
No full textAlt L, Thiery S. Gender in Schule und Didaktik. In: Schößler F, Wille L, eds. Einführung in die Gender Studies. Berlin; 2022: 103-117
Software Review for the Software PointSamplingTool
No full textDie Early Views der Archäologischen Informationen: Rezensionen Archäoinformatik
Thiery, F., Homburg, T., Klammt, A. & Schmidt, S. C. (2022). Software Review for the Software PointSamplingTool. Archäologische Informationen 44, Early View, published online 18 Nov 2022. [PDF
[postcard] Exposition universelle et internationale de Gand (Belgique). avril - octobre 1913. Henri Thiery.
No full textEuropeana-wt1913Bijzondere collectie
[postcard] Exposition universelle et internationale de Gand (Belgique). Avril - octobre 1913. Henri Thiery.
No full textDonkerblauwEuropeana-wt1913Bijzondere collectie
The labeling system: A new approach to overcome the vocabulary bottleneck
No full textShared controlled vocabularies are a prerequisite for collaborative annotation and semantic interchange. The creation and maintenance of such vocabularies is, however, time-consuming and expensive. The diversity of research questions in the humanities makes it virtually impossible to create shared controlled vocabularies that cover a wide range of potential applications and satisfy the needs of diverse stakeholders. In this paper we present a novel conceptual approach for mitigating these problems. We propose that projects define their own vocabularies as needed and link the vocabulary terms to one or more concepts in a reference thesaurus, so that the project-specific term effectively serves as a "label" for a set of shared concepts. We also describe the implementation of this approach in the Labeling System. The Labeling System is a Web application that allows users to easily import concepts or create SKOS vocabularies and link the vocabulary terms to concepts from one or more reference thesauri
Mémoire sur l'amélioration des chevaux dans les deux départemens du Rhin... / par P.-J. Thiery,...
No full textAppartient à l’ensemble documentaire : Alsace1Contient une table des matièresAvec mode text
Cloning and characterization of three Xenopus Slug promoters reveal direct regulation by Lef/beta-catenin signaling
No full textIn amphibians and birds, one of the first steps of neural crest cell (NCC) determination is expression of the transcription factor Slug. This marker has been used to demonstrate that BMP and Wnt molecules play a major role in NCC induction. However, it is unknown whether Slug expression is directly or indirectly regulated by these signals. We report here the cloning and characterization of three Xenopus Slug promoters: that of the Xenopus tropicalis slug gene and those of two Xenopus laevis Slug pseudoalleles. Although the three genes encode proteins with almost identical amino acid sequences and are expressed with similar spatiotemporal patterns, their 5'-flanking regions are quite different. A striking difference is a deletion in the X. tropicalis gene located precisely at the transcription initiation site that results in the X. tropicalis promoter being inefficient in X. laevis. Additionally, we identified two regions common to the three promoters that are necessary and sufficient to drive specific expression in NCCs. Interestingly, one of the common regulatory regions presents a functional Lef/beta-catenin-binding site necessary for specific expression. As the Lef.beta-catenin complex is a downstream effector of Wnt signaling, these results suggest that Xenopus Slug is a direct target of NCC determination signals
Dissertatio de exstirpatione uteri, quam pro summis in arte medica honoribus rite Obtinendis... /
No full textHerkomst: Collectie Michel Thiery, Het Pand, mei 202
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